Micellar curcumin improves the antibacterial activity of the alkylphosphocholines erufosine and miltefosine against pathogenicStaphyloccocus aureusstrains

Abstract

2019) Micellar curcumin improves the antibacterial activity of the alkylphosphocholines erufosine and miltefosine against pathogenic Staphyloccocusaureus strains, Biotechnology & Biotechnological Equipment, 33:1,[38][39][40][41][42][43][44][45][46][47][48][49][50][51][52][53] ABSTRACT In the light of the emerging bacterial resistance to broad-spectrum antibiotics, the search for new antibacterial therapeutics and drug combinations is one of the most challenging topics nowadays. In the present study, we investigated for the first time the antibacterial and biofilm inhibitory effects of the third generation anticancer alkylphosphocholine (APC) erufosine against pathogenic Staphylococcus aureus strains in comparison to the prototype of this pharmacological class of drugs, miltefosine. We also searched for synergistic antibacterial combinations between both APCs and curcumin incorporated in copolymeric micelles based on PluronicV R P123 or a mixture of Pluronic V R P123 and Pluronic V R F127 (P123/F127). The obtained quantitative redoxactivity experimental data and drug-drug interactions were evaluated by using mathematical models in the MAPLE software. Similar to miltefosine, erufosine showed a moderate bacteriostatic effect in clinically relevant concentrations (50 Ä 60 lmol/L) and inhibited the redox activity of the treated bacteria up to 90% at minimal inhibitory concentrations. The effect of both APCs towards methicillin resistant staphylococci was enhanced by combinations with P123/F127 micellar CRM at a ratio of 1:1. Erufosine showed a stronger median biofilm inhibition at lower concentrations (MBIC 50 ¼ 1.87 lmol/L) than miltefosine (MBIC 50 ¼ 6.0 lmol/L) and curcumin (MBIC 50 ¼ 24.84 lmol/L) as demonstrated by quantification of biofilm-bound bacteria. In conclusion, the estimated antibacterial activity of erufosine widens the spectrum of its useful pharmacological effects, which is important for its clinical development. The established synergistic and additive drug combinations could be beneficial for the application of both APCs in cancer therapy, since numerous malignancies are accompanied by bacterial infections. ARTICLE HISTORY

Nanocurcumin: A Promising Candidate for Therapeutic Applications

“…It was found that silverdecorated polymeric micelles encapsulated with curcumin exhibited strong antibacterial activity to P. aeruginosa and Staphylococcus aures (Huang et al, 2017). Similarly, Zaharieva et al reported that curcumin loaded micelles enhance the alkylphosphocholines erufosine and miltefosine antibacterial activities against pathogenic S. aureus strain (Zaharieva et al, 2019). Wang et al demonstrated that encapsulated curcumin exhibited a broad spectrum of antifungal activity by obstructing S. cerevisiae, Aspergillus niger, and Penicillium notatum.…”

Improvement of the Antimicrobial Activity of Oregano Oil by Encapsulation in Chitosan—Alginate Nanoparticles

“…The quantitative evaluation of the inhibition effects of OrO and OrO-NP on the test microbial strains was performed using the Lambert-Pearson (LP) model considering the inhibitory effect of the applied concentration and fitting the experimental data on the base of the weighted least squares statistical method [79,80]. The program was coded in the MAPLE symbolic mathematics software.…”

Quorum quenching of Streptococcus mutans via the nano-quercetin-based antimicrobial photodynamic therapy as a potential target for cariogenic biofilm

“…The attached bacterial cells were then stained with 0.1% crystal violet for 15 min and dissolved in 96% ethanol to remove the bound dye from the stained cells. The dye bound to biofilm was re-solubilized with 150 μL of 33% acetic acid and the optical density (OD) of each well was measured at 570 nm by a microplate reader (BioTek, Germany) [ 23 ]. The following controls were included in each MBIC test plate: 1) 0.2% chlorhexidine (CHX; positive control); 2) S. mutans in medium (negative control); 3) medium only (sterility control).…”