Micellar electrokinetic capillary chromatography as a powerful tool for pharmacological investigations without sample pretreatment: A precise technique providing cost advantages and limits of detection to the low nanomolar range

Capillary electrophoresis analysis of biofluids with a focus on less commonly analyzed matrices

“…One way to avoid adsorption of matrix proteins which has long been used in liquid chromatography is to complex them with a surfactant such as sodium dodecyl sulfate (SDS) in the mobile phase [26]. Analogous to this, SDS can be used as a component of the CE BGE to effectively reduce protein adsorption [7,27]. Although first applied [28] and later optimized [29][30][31] for direct injection of plasma, this approach may be used in the analysis of a variety of biofluids and several direct injection assays using surfactant additives are discussed in the following sections dealing with specific matrices.…”

Precision in affinity capillary electrophoresis for drug–protein binding studies

“…From earlier works rinsing regimes are known to wash protein off the uncoated capillary making use of SDS containing buffers [35,36]. In the present work, we also tried to use SDS rinsing procedures ( Fig.…”

Advances of capillary electrophoresis in clinical and forensic analysis (1999-2000)

“…In the covered period, a large number of comprehensive reviews on selected topics, including those on general aspects of bioanalysis of drugs [54], monitoring of drugs in serum [55], analysis of illicit and abused drugs in urine [56], screening of biological specimens for drugs of forensic interest [57], enantiospecific drug analysis in biological samples [58], enantiospecific applications in drug metabolism and pharmacokinetics [59], pharmacokinetic applications [60], CE-LIF in clinical drug development [61], CE-based immunoassays [62], microdialysis as a tool for pharmacokinetics [63] and strategies to improve the sensitivity in CE for analysis of drugs in body fluids [64], appeared in the literature. Furthermore, Thormann et al [65] described a family of multianalyte CE-based assays for screening and confirmation of urinary drugs of abuse that includes immunoassays, CE with multiwavelength absorption detection and CE-MS, Kunkel and Wätzig [66] praised MEKC as a powerful tool for pharmacological investigations without sample pretreatment and Govindaraju and Lloyd [67] highlighted the use of CE for analysis of small-scale biological compartments. A current contents search revealed only few new papers in the area of therapeutic drug monitoring, a somewhat higher use of CE for toxicology, an increased employment of CE for assessment of drug metabolism and many papers showing the feasibility of monitoring specific drugs in biological samples.…”