Micellar electrokinetic capillary chromatography method for direct determination of glucuronides of entacapone and its (Z)-isomer in human urine

Chromatographic separation of (E)- and (Z)-isomers of entacapone and their simultaneous quantitation in human plasma by LC–ESI-MS/MS

“…The chromatographic separation was achieved in a run time of 20 min and the quantitation was possible over a wide dynamic range of 0.0025-100 g/mL. Two methods based on micellar electrokinetic capillary chromatography have been developed by Lehtonen et al [10,11] for the separation of two main urinary metabolites of entacapone in humans. The electropherograms in both these methods required 18 min for complete resolution of 3-O-glucuronide isomers (E and Z) of entacapone.…”

Determination of Antiparkinsonism Drug Entacapone

“…In the first method good separation of glucuronides was achieved with 20mM-ammonium acetate as separation solution at pH 6.84 3 . In the second method detection was achieved at 335nm 4 Three HPLC methods for the determination of entacapone were reported, the first one determine entacapone and its Z-isomer in human plasma and urine on LiChrosorb C18 column fitted with a micro Bondapak C-18 guard column 5 .…”