Micellar electrokinetic chromatography: A new simple tool for the analysis of synthetic cannabinoids in herbal blends and for the rapid estimation of their logP values

Gottardo, Rossella; Bertaso, Anna; Pascali, Jennifer P.; Sorio, Daniela; Musile, Giacomo; Trapani, Elisa; Seri, Catia; Serpelloni, Giovanni; Tagliaro, Franco

doi:10.1016/j.chroma.2012.08.085

Cited by 34 publications

(22 citation statements)

References 29 publications

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“…The most widely used organic modifier in MEKC is acetonitrile, which is particularly effective in reducing retention factors and expanding the migration window [20]. Hence, acetonitrile ranging from 0% to 15% was chosen to be the organic modifier in this study.…”

Section: Resultsmentioning

confidence: 99%

The Activity-Integrated Method for Quality Assessment of Reduning Injection by On-Line DPPH-CE-DAD

et al. 2014

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A sensitive on-line DPPH-CE-DAD method was developed and validated for both screening and determining the concentration of seven antioxidants of Reduning injection. The pH and concentrations of buffer solution, SDS, β-CD and organic modifier were studied for the detection of DPPH and seven antioxidants. By on-line mixing DPPH and sample solution, a DPPH-CE method for testing the antioxidant activity of the complex matrix was successfully established and used to screen the antioxidant components of Reduning injection. Then, antioxidant components including caffeic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, chlorogenic acid, neochlorogenic acid and cryptochlorogenic acid were quantified by the newly established CE–DAD method. Finally, the total antioxidant activity and the multiple active components were selected as markers to evaluate the quality of Reduning injection. The results demonstrated that the on-line DPPH-CE-DAD method was reagent-saving, rapid and feasible for on-line simultaneous determination of total pharmacological activity and contents of multi-components samples. It was also a powerful method for evaluating the quality control and mechanism of action of TCM injection.

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Section: Resultsmentioning

confidence: 99%

The Activity-Integrated Method for Quality Assessment of Reduning Injection by On-Line DPPH-CE-DAD

et al. 2014

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“…Acetonitrile was the most widely used organic modifier in CE [22]. Acetonitrile ranging from 0% to 15% was investigated.…”

Section: Resultsmentioning

confidence: 99%

A Green Antioxidant Activity‐Integrated Dual‐Standard Method for Rapid Evaluation of the Quality of Traditional Chinese Medicine Xuebijing Injection by On‐Line DPPH‐CE‐DAD

Liu

et al. 2016

Evidence-Based Complementary and Alternative Medicine

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Much attention has been focused on treatment of sepsis which leads to high mortality all over the world in every year. Antioxidant activity seems to play a prominent role in the treatment of sepsis exhibited by Xuebijing injection. The aim of the present research was to develop an on-line 1, 1-diphenyl-2-picrylhydrazyl- (DPPH-) capillary electrophoresis-diode array detector (on-line DPPH-CE-DAD) method for rapidly assessing antioxidant properties and efficacious material basis of antioxidant activity as a way of quality control of Xuebijing injection. Several parameters affecting the separation were investigated, including the pH and concentrations of buffer, SDS, β-CD, and organic modifier as well as voltage and cassette temperature. Compared to previous traditional method, this improved method shortened the experimental cycle and became more efficient because it was successfully applied to analyze total antioxidant activity and contents of twelve antioxidants of Xuebijing injection under the same condition. The results revealed that the on-line DPPH-CE-DAD method was a reagent-saving, rapid, feasible, and green technique for quality control of Xuebijing injection in terms of pharmacological activity and contents of active ingredients. It also offered new opportunities for the analysis of antioxidant activity of complex matrix.

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“…Abbreviations: APFOA, ammonium perfluorooctanoate; IS, internal standard; MRM, multiple-reaction monitoring; PFOA, perfluorooctanoic acid cost, and short preconditioning time [10][11][12]. Furthermore, the hyphenation of CE with MS offers high sensitivity and the possibility of structurally characterizing the analyte, as well as several other advantages of CE [7,[13][14][15].…”

Section: Introductionmentioning

confidence: 98%

MEKC-MS/MS method using a volatile surfactant for the simultaneous determination of 12 synthetic cannabinoids

Akamatsu

Mitsuhashi

2013

J. Sep. Science

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This study describes a method for the simultaneous determination of 12 synthetic cannabinoids by MEKC-MS/MS using a volatile surfactant (ammonium perfluorooctanoate) as a constituent of the micellar pseudostationary phase. Although most synthetic cannabinoids comigrated by a CZE method, sufficient separation could be achieved by the proposed method. The best separation was made possible by 50 mM ammonium perfluorooctanoate in 20% v/v acetonitrile/water (apparent pH* 9.0) as the BGE, followed by MS detection using a sheath liquid composed of 5 mM ammonium formate in 50% v/v methanol/water mixed hydro-organic solvent. The standard calibration curve for all analytes showed good linearity (r > 0.99). Satisfactory recoveries, ranging from 89.5 to 101.7%, were obtained. The LODs were 6.5-76.5 μg/g for the target analytes. This method appears to be a useful tool for the identification of synthetic cannabinoids in illegal herbal incense blends.

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Micellar electrokinetic chromatography: A new simple tool for the analysis of synthetic cannabinoids in herbal blends and for the rapid estimation of their logP values

Cited by 34 publications

References 29 publications

The Activity-Integrated Method for Quality Assessment of Reduning Injection by On-Line DPPH-CE-DAD

The Activity-Integrated Method for Quality Assessment of Reduning Injection by On-Line DPPH-CE-DAD

A Green Antioxidant Activity‐Integrated Dual‐Standard Method for Rapid Evaluation of the Quality of Traditional Chinese Medicine Xuebijing Injection by On‐Line DPPH‐CE‐DAD

MEKC-MS/MS method using a volatile surfactant for the simultaneous determination of 12 synthetic cannabinoids

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