(Micellar) electrokinetic chromatography: an interesting solution for the liquid phase separation dilemma

Welsch, Thomas; Michalke, Daniela

doi:10.1016/s0021-9673(03)00503-x

Cited by 19 publications

(11 citation statements)

References 147 publications

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“…Capillary EKC (CEKC), namely its micellar mode (CMEKC), last year celebrating 20 years of its invention by Terabe et al [135] and on this occasion reviewed by him [136] and other authors [137,138]. CMEKC is mostly used with ionogenic detergents, anionic SDS, cationic CTAB, or zwitterionic CHAPS; it is suitable for separation of electroneutral peptides, i.e., peptides with blocked or derivatized N-and C-termini and other ionogenic groups of peptide chain, and/or for separation of peptides having the same or very similar charge-to-mass ratio but differing in their hydrophobicity.…”

Section: Electrokinetic Chromatographymentioning

confidence: 99%

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

2006

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The article gives a comprehensive review on the recent developments in the applications of high-performance capillary electromigration methods, zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography, to analysis, preparation, and physicochemical characterization of peptides. The article presents new approaches to the theoretical description and experimental verification of electromigration behavior of peptides, covers the methodological aspects of capillary electroseparations of peptides, such as rational selection of separation conditions, sample preparation, suppression of peptide adsorption, new developments in individual separation modes, and new designs of detection systems. Several types of applications of capillary electromigration methods to peptide analysis are presented: conventional qualitative and quantitative analysis, purity control, determination in biomatrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid and sequence analysis, and peptide mapping of proteins. Some examples of micropreparative peptide separations are given and capabilities of capillary electromigration techniques to provide important physicochemical characteristics of peptides are demonstrated.

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Section: Electrokinetic Chromatographymentioning

confidence: 99%

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

2006

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“…This is not easily achievable in HPLC. Several workers have demonstrated the ease and flexibility of the MEKC for analysis of neutral and weakly charged compounds [1][2][3][4]. Here, we demonstrate how direct sample injection without any treatment can be applied to the routine analysis of iohexol in serum for assessing the glomerular filtration rate (GFR) and with very good daily reproducibility.…”

Section: Introductionmentioning

confidence: 86%

Serum iohexol analysis by micellar electrokinetic capillary chromatography

Shihabi

Hinsdale

2006

Electrophoresis

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A simple and rapid ( approximately 4 min) method for the measurement of iohexol in serum for assessing the glomerular filtration rate is described. It is based on direct serum injection on the capillary by MEKC. The method is linear between 8 and 260 mg/L, with an RSD of peak height of 2.9%. Several simple steps have contributed to an improved daily precision, such as choosing a high pH buffer, increasing the SDS concentration, frequent standardization, and eliminating any sample pretreatment.

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“…The use of high‐performance LC (HPLC) enables the separation of a larger number of isomers than GC []. HPLC is a well‐established separation technique, even though it is characterized by relatively low efficiency due to the small diffusion coefficients of the analytes in the liquid phase, as well as in the stationary phase []. In recent years, the reduction of the particle size ( d p ) has acted as an important driving force for improvement in the HPLC field.…”

Section: Introductionmentioning

confidence: 99%

Ultra‐high performance liquid chromatographic method for the determination of polycyclic aromatic hydrocarbons in a passive environmental sampler

Purcaro

Moret

Bučar-Miklavčić³

2012

J of Separation Science

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Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous compounds released in the environment by different sources. The aim of the present work was to validate a solid-phase extraction (SPE) and a rapid ultra-high performance liquid chromatographic (UHPLC) method for the analysis of PAHs in a passive environmental sampler, namely a Dacron® (the commercial name of a synthetic fiber based on polyethylene terephthalate) textile. The elution temperature was optimized to improve the resolution of early-eluted compounds, namely acenaphthene (Ac) and fluorene (F). The UHPLC method lasts about 10 min and showed good linearity for all the 16 PAHs considered, with regression coefficients over 0.99. Recoveries, limits of detection (LODs), and limits of quantification (LOQs) of the SPE method were well within the performance criteria fixed by the Regulation n. 836/2011, namely 0.3 and 0.9 μg/kg, respectively.

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(Micellar) electrokinetic chromatography: an interesting solution for the liquid phase separation dilemma

Cited by 19 publications

References 147 publications

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

Serum iohexol analysis by micellar electrokinetic capillary chromatography

Ultra‐high performance liquid chromatographic method for the determination of polycyclic aromatic hydrocarbons in a passive environmental sampler

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