Micellar electrokinetic chromatography fingerprinting combined with chemometrics as an efficient strategy for evaluating the quality consistency and predicting the antioxidant activity of Lianqiao Baidu pills

Abstract

An approach combining micellar electrokinetic chromatography fingerprinting with chemometrics was developed to evaluate the quality consistency of Lianqiao Baidu pills, which are traditional Chinese patent medicines composed of 19 herbs used mainly to treat skin ulcers, common cold, rheumatism, herpes, and constipation. The triangle optimization method was employed to choose a satisfactory background electrolyte, with the information index, I, as an objective function for assessing the capillary electrophoresis conditions. Then, under the optimal conditions, the micellar electrokinetic chromatography fingerprints of 28 batches of samples were established, and five marker compounds were quantitatively determined simultaneously. A limited-ratio quantified fingerprint method was introduced to evaluate the chromatographic fingerprints both qualitatively and quantitatively. Principle component analysis revealed that the 28 batches of samples can be clustered according to different manufacturers. Moreover, the relationship between the fingerprint and the antioxidant activity was explored by orthogonal partial least-squares regression, which provided critical medicinal efficacy information for quality control. The present study establishes a powerful and reliable method for monitoring the quality consistency of Lianqiao Baidu pill.

Comprehensively evaluating the quality consistency of Forsythia suspensa by five-wavelength mean fusion fingerprint profiling combined with spectrum quantum fingerprint and antioxidant activity

“…Ratio fingerprint is proposed to calculate the relative percentage of each fingerprint component to the standard fingerprint, with more emphasis on the relative percentage control of each fingerprint. The limit ratio quantified fingerprint method 24 and the simple ratio quantified fingerprint method 25 were combined due to the large error caused by the outliers’ sudden jump and the variability of the ratio fingerprint values. With integrated macro-qualitative similarity ( S ms ) and integrated macro-quantitative similarity ( P ms ), the MQCRF method can control sample fingerprints qualitatively and quantitatively.…”

Assessment of quality consistency in traditional Chinese medicine using multi‐wavelength fusion profiling by integrated quantitative fingerprint method: Niuhuang Jiedu pill as an example

“…The cosine of the two vectors after conversion is defined as the qualitative limited ratio similarity (), as calculated by Eq. . Integrated qualitative similarity () is obtained by averaging , and as shown in Eq.…”

In‐capillary screening and quantifying the antioxidants of Yangxinshi Tablet using field‐enhanced sample injection‐micellar electrokinetic chromatography

“…Therein, MEKC is usually used as a common mode because of its good characteristic of separation. A number of researchers successively established the MEKC methods combining with antioxidative investigations for performing an effective quality evaluation of TCMP [24][25][26]. However, they required additional instruments and failed to realize the integration of activity and content.…”