Micellar liquid chromatography: suitable technique for screening analysis

Recent Advances in the Analysis of Steroid Hormones and Related Drugs

“…167 The use of micellar liquid chromatography, a special branch of RP-HPLC where the eluent contains very low concentration of organic modifiers (mainly 1-pentanol and acetonitrile) and about 0.1 M sodium dodecyl sulfate (SDS) as the micelle forming agent was applied to steroid analysis by Spanish groups. [168][169][170][171][172][173][174][175][176] This technique enables the relatively rapid analysis of derivatives with wide range of polarity with minimum need for sample preparation. In addition to retentionstructure relationship studies 169 and screening of steroids, [170][171][172] the method was successfully used for the determination of spironolactone, 168 corticosteroids, 173,176 methyltestosterone, 174 anabolic steroids 176 and danazol 175 in various formulations.…”

Simultaneous determination of 16 estrogens, dehydroepiandrosterone and their glucuronide and sulfate conjugates in serum using sodium cholate micelle capillary electrophoresis

“…A borate-phosphate buffer (pH 8.0) with sodium cholate was reported for the determination of estrogen (Imawzawa and Hatanaka, 1997; Kobayashi et al, 1995;Ruiz- Electropherograms of steroids and metabolites using pH 7.0-10.0 buffers. Each steroid (10 mg/mL) was separated on pH 7.0-10.0.…”

“…1, estrone, E1; 2, estradiol, E2; 3, estriol, E3; 4, estrone gluculonide, E1-G; 5, estradiol-3-glucuronide, E2-3G; 6, estradiol-17-glucuronide, E2-17G; 7, estradiol-3-glucuronide-17-sulfate, E2-3G-17S; 8, estradiol-3-sulfate, E2-3S; 9, estradiol-3,17-sulfate, E2-3, 17S; 10, estriol-3-glucuronide, E3-3G; 11, estriol-16-gluculonide, E3-16G; 12, 2-hydroxyestradiol, E2-20H; 13, 4-hydroxyestradiol, E2-4OH; 14, DHEA; 15, DHEA-S; 16, 3-methyltestosterone, 3-MeT. Angel et al, 2002) by MEKC. A borate buffer with SDS and g-cyclodextrin was also reported for the determination of phenols (Takeda et al, 2000) and estrogen by MEKC (Ji et al, 1995).…”

“…Each steroid is monitored individually by immunoassay methods in many clinical laboratories (Goharkhay et al, 2001;Manolopoulos et al, 2001;Mantzavinous et al, 1991). The chromatographic methods, gas chromatography (GC; Pinnella et al, 2001), gas chromatography-mass spectrometry (GC-MS; Raju et al, 2000;Tagawa et al, 2000), high performance liquid chromatography (HPLC; Hampl et al, 2000;Le Curieux-Belfond et al, 2000;Yasui et al, 2001) and capillary electrophoresis (CE; Imazawa and Hatanaka, 1997;Ji et al, 1995;Kobayashi et al, 1995;Ruiz-Angel et al, 2002) have been reported for simultaneous determination of these steroids. In these chromatographic methods, target steroids were free (unconjugated) types.…”

“…Therefore, we developed a method for the simultaneous determination of free and conjugated steroids. CE is a relatively new analytical method for the determination of estrogen (Ji et al, 1995;Kobayashi et al, 1995;Ruiz-Angel et al, 2002), progesterone, testosterone and DHEA-S (Abubaker et al, 1995;Kobayashi et al, 1995;Quirino et al, 1998) in serum and urine samples using micellar electrokinetic chromatography (MEKC). MEKC has high separation efficiency, but there have been few reports of simultaneous determination of free and conjugated steroids.…”