“…Although many assays use RT-qPCR for the diagnosis of COVID-19 [ 1 , 2 , 29 , 30 , 31 , 32 , 33 ], a droplet digital polymerase chain reaction (ddPCR) is newer quantitative PCR technology. Available since 2011, ddPCR allows for the absolute quantification of nucleic acid [ 27 , 34 , 35 ] without the need for a standard curve [ 29 , 30 , 32 , 33 , 35 ]. Multiple studies have compared ddPCR with RT-qPCR and have consistently found ddPCR to be more sensitive, specific, and repeatable [ 1 , 2 , 3 , 28 , 29 , 30 , 31 , 32 , 35 , 36 , 37 , 38 ].…”