2022
DOI: 10.1016/bs.pmbts.2021.07.020
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Micro and nanofluidics for high throughput drug screening

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Cited by 4 publications
(4 citation statements)
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“…Although many assays use RT-qPCR for the diagnosis of COVID-19 [ 1 , 2 , 29 , 30 , 31 , 32 , 33 ], a droplet digital polymerase chain reaction (ddPCR) is newer quantitative PCR technology. Available since 2011, ddPCR allows for the absolute quantification of nucleic acid [ 27 , 34 , 35 ] without the need for a standard curve [ 29 , 30 , 32 , 33 , 35 ]. Multiple studies have compared ddPCR with RT-qPCR and have consistently found ddPCR to be more sensitive, specific, and repeatable [ 1 , 2 , 3 , 28 , 29 , 30 , 31 , 32 , 35 , 36 , 37 , 38 ].…”
Section: Introductionmentioning
confidence: 99%
“…Although many assays use RT-qPCR for the diagnosis of COVID-19 [ 1 , 2 , 29 , 30 , 31 , 32 , 33 ], a droplet digital polymerase chain reaction (ddPCR) is newer quantitative PCR technology. Available since 2011, ddPCR allows for the absolute quantification of nucleic acid [ 27 , 34 , 35 ] without the need for a standard curve [ 29 , 30 , 32 , 33 , 35 ]. Multiple studies have compared ddPCR with RT-qPCR and have consistently found ddPCR to be more sensitive, specific, and repeatable [ 1 , 2 , 3 , 28 , 29 , 30 , 31 , 32 , 35 , 36 , 37 , 38 ].…”
Section: Introductionmentioning
confidence: 99%
“…This feature allows for direct and independent DNA quantification, providing more precise and reproducible data with a strong anti-interference ability [8]. Furthermore, ddPCR has proven to be an effective method for detecting low-abundance targets [9], which holds great potential in pathogen detection applications [5,[10][11][12][13]. The emergence of ddPCR has greatly improved the accuracy and sensitivity of nucleic acid detection.…”
Section: Introductionmentioning
confidence: 99%
“…Microfluidic devices can be utilized for high-throughput drug screening, cell culture, DNA sequencing and point-of-care diagnostics. These devices and systems have advantages over standard laboratory procedures, such as reduced sample volume, increased sensitivity and enhanced accuracy [15].…”
Section: Introductionmentioning
confidence: 99%