Micro Flow-Through Thermocycler with Simple Meandering Channel with Symmetric Temperature Zones for Disposable PCR-Devices in Microscope Slide Format

Reichert, Anett; Felbel, Jana; Kielpinski, Mark; Urban, Matthias; Steinbrecht, B.; Henkel, Thomas

doi:10.1016/s1672-6529(08)60173-0

Cited by 17 publications

(9 citation statements)

References 12 publications

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“…Examples for injection compression molding can be found elsewhere. 94 The most important thermoplastic polymers for microfluidics are compared in Table 1. Thermoplasts are typically polymerized from their respective monomer:…”

Section: Physical Classification Of Polymersmentioning

confidence: 99%

Let there be chip—towards rapid prototyping of microfluidic devices: one-step manufacturing processes

et al. 2011

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Section: Physical Classification Of Polymersmentioning

confidence: 99%

Let there be chip—towards rapid prototyping of microfluidic devices: one-step manufacturing processes

et al. 2011

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“…14 PCR is a simple reaction that is a cost-effective choice for laboratories because there are several automated instruments available along with a range of options for real-time detection chemistries. 15 A large number of microsystems for nucleic acid amplification using PCR have been developed since the first by Northrup et al in 1993 16 including several recent examples, [17][18][19][20][21][22][23][24] some at various stages of integration with sample preparation and detection on the same chip. Impressive limits of detection have been achieved and rapid amplification times down to a few minutes have been realized by using microfluidics to optimize thermal cycling.…”

Section: Introductionmentioning

confidence: 99%

Miniaturized isothermal nucleic acid amplification, a review

2011

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Micro-Total Analysis Systems (µTAS) for use in on-site rapid detection of DNA or RNA are increasingly being developed. Here, amplification of the target sequence is key to increasing sensitivity, enabling single-cell and few-copy nucleic acid detection. The several advantages to miniaturizing amplification reactions and coupling them with sample preparation and detection on the same chip are well known and include fewer manual steps, preventing contamination, and significantly reducing the volume of expensive reagents. To-date, the majority of miniaturized systems for nucleic acid analysis have used the polymerase chain reaction (PCR) for amplification and those systems are covered in previous reviews. This review provides a thorough overview of miniaturized analysis systems using alternatives to PCR, specifically isothermal amplification reactions. With no need for thermal cycling, isothermal microsystems can be designed to be simple and low-energy consuming and therefore may outperform PCR in portable, battery-operated detection systems in the future. The main isothermal methods as miniaturized systems reviewed here include nucleic acid sequence-based amplification (NASBA), loop-mediated isothermal amplification (LAMP), helicase-dependent amplification (HDA), rolling circle amplification (RCA), and strand displacement amplification (SDA). Also, important design criteria for the miniaturized devices are discussed. Finally, the potential of miniaturization of some new isothermal methods such as the exponential amplification reaction (EXPAR), isothermal and chimeric primer-initiated amplification of nucleic acids (ICANs), signal-mediated amplification of RNA technology (SMART) and others is presented.

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“…Platforms with integrated solid phase PCR (Hung et al ., 2017), or integrated isolation of pathogens (Sandetskaya et al ., 2017), and highly sensitive PCR‐chips have been developed using flow‐through PCR‐systems. Thereby the PCR fluid is guided through microchannels that are integrated into a chip with different temperature profiles (Schneegass et al ., 2001; Reichert et al ., 2008; Markey et al ., 2010; Ahrberg et al ., 2016; Liu et al ., 2018). Other miniaturized technologies work in a stationary regime: As film‐based PCR chip (Bae et al ., 2018), or the reaction mixture is put as droplet onto the chip surface (Guttenberg et al ., 2005) before heated to realize the needed temperature regime.…”

Section: Introductionmentioning

confidence: 99%

Chip‐based duplex real‐time PCR for water quality monitoring concerning Legionella pneumophila and Legionella spp.

Reuter

Hentschel

Breitenstein

et al. 2020

Water & Environment J

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Based on biomolecular methods, rapid and selective identification of human pathogenic water organisms becomes an important issue. Legionella spp., are pathogenic water bacteria with worldwide significance. Prevalent detection methods for these microorganisms are time and/or cost intensive. We describe a detection setup and relating DNA assay. A miniaturized real‐time polymerase chain reaction (real‐time PCR) for direct on‐line discrimination of Legionella pneumophila and Legionella spp. was established and integrated into a real‐time PCR‐chip‐system. The PCR‐chip device combines a temperature controlling unit and a fluorescence intensity measurement. It was designed to achieve rapid amplification, using an approach of real‐time fluorescence read out with the intercalating dye EvaGreen® and melting curve analysis, without requiring multiple probes. The presented results exhibit reproducibility and good sensitivity, showing that the setup is suitable for robust, rapid and cost‐efficient detection and monitoring of a variety of Legionella spp.in urban water samples.

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Micro Flow-Through Thermocycler with Simple Meandering Channel with Symmetric Temperature Zones for Disposable PCR-Devices in Microscope Slide Format

Cited by 17 publications

References 12 publications

Let there be chip—towards rapid prototyping of microfluidic devices: one-step manufacturing processes

Let there be chip—towards rapid prototyping of microfluidic devices: one-step manufacturing processes

Miniaturized isothermal nucleic acid amplification, a review

Chip‐based duplex real‐time PCR for water quality monitoring concerning Legionella pneumophila and Legionella spp.

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