Micro isoelectric focusing in polyacrylamide gel columns

Phospholipase C from Bacillus cereus and Its Use in Studies of Tissue Thromboplastin

“…Gradients of pH 3-10 and pH 5-8 were used. Isoelectric focusing in gel was carried out according to Catsimpoolas [12].…”

Chemical Modification of Sulfhydryl Groups in Porcine Pancreaticα-Amylase, and Purification and Properties of the Modified Amylase

“…PAGE and SDS-PAGE were performed according to the method of Davis 10 ) and the method of Weber and Osborn,l1) respectively. Polyacrylamide gel isoelectric focusing was performed with 2% ampholine (pH 3.5", 10.0) according to the method of Catsimpoolas 12 ) with an acrylarpide concentration of 5%. Protein bands were stained with Coomassie Brilliant Blue G-250.…”

Isoelectric focusing of fibrinogens on polyacrylamide gels

“…Electrophoresis was continued for 24 hr, during which time the current decreased below 0.3 mA per gel. Gels were stained according to the method described by Awdeh [5] and the pH gradients in gels determined as described [8,9]. Immunological experiments employed Catsimpoolas' sectional immuno-electrofocusing technique [6] using Ouchterlony plates (1% agarose, 0.1 M tris pH 7.4) and sheep antisera against rabbit fibrinogen and rabbit serum proteins [7].…”