Micro-MRI methods to detect renal cysts in mice

Kobayashi, Hisataka; Kawamoto, Satomi; Brechbiel, Martin W.; Jo, Sang Kyung; Hu, Xiaobang; Yang, Tianxin; Diwan, Bhalchandra A.; Waldmann, Thomas A.; Schnermann, Jürgen; Choyke, Peter L.; Star, Robert A.

doi:10.1111/j.1523-1755.2004.00532.x

Cited by 26 publications

(18 citation statements)

References 14 publications

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“…It is important that the dendrimer-contrast agent is used by other investigators with different models of ARF (and chronic renal failure [23]) both to confirm our findings and to produce further insights into the pathophysiology of renal injury. Obviously, the next step is to use this contrast agent in humans – both with normal renal function and with renal injury.…”

Section: Introductionsupporting

confidence: 50%

Imaging Acute Renal Failure with Polyamine Dendrimer-Based MRI Contrast Agents

et al. 2006

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Acute renal failure (ARF) induced by sepsis has a high mortality but lacks effective treatments. To develop novel therapies we must diagnose renal injury early and accurately in septic patients and identify any additional insults such as nephrotoxic drugs and ischemia. In this short review we describe our experience using MRI with dendrimer-based contrast agents in mouse models of ARF. This technique can diagnose early renal injury before serum creatinine is elevated, distinguish different ARF etiologies, track drug therapy and predict outcome. As an ARF biomarker, MRI with dendrimer-based contrast is a promising technique deserving further development.

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Section: Introductionsupporting

confidence: 50%

Imaging Acute Renal Failure with Polyamine Dendrimer-Based MRI Contrast Agents

et al. 2006

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“…Recent studies have illustrated the potential of MR to elucidate the development of renal cysts in the course of ADPKD in clinical studies [14,15] and also in animal models of cystic diseases [4,12,13,18]. Recently, Wallace et al showed that in vivo measurements of kidney volume using a dedicated animal scanner can accurately monitor the increase of renal volume in disease models [18].…”

Section: Discussionmentioning

confidence: 98%

“…MRI has already proven to be a reliable method to measure renal volumetric indices in preclinical [4,12,13] and clinical studies [14][15][16][17]. Therefore, MRI-based volume measurements can be used as a reliable medium to detect the progression of the disease.…”

Section: Introductionmentioning

confidence: 99%

Monitoring kidney and renal cyst volumes applying MR approaches on a rapamycin treated mouse model of ADPKD

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Becker

et al. 2008

Magn Reson Mater Phy

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“…Using the renal suction method, transgene expression was observed to a depth of at least 700 lm (Figure 3(A)). The total thickness of the renal cortex in mice is reported to be about 1 mm from the surface (Zhai et al, 2003;Kobayashi et al, 2004). The depth of transgene expression using the renal suction method could be sufficient to deliver pDNA to the deep cortex of the kidney.…”

Section: Discussionmentioning

confidence: 99%

Characterization of transgene expression and pDNA distribution of the suctioned kidney in mice

et al. 2017

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We have previously developed an efficient and safe transfection method for the kidney in mice: renal suction-mediated transfection. In this study, we verified the detailed characteristics of transgene expression and plasmid DNA (pDNA) in mice to develop therapeutic strategies and application to gene function analysis in the kidney. After naked pDNA was administered intravenously, the right kidney was immediately suctioned by a tissue suction device. We examined the spatial distribution of transgene expression and pDNA in the suctioned kidney using tissue clearing by CUBIC, Clear T2 , and Scale SQ reagents. Spatial distribution analysis showed that pDNA was transfected into extravascular cells and sufficiently delivered to the deep renal cortex. In addition, we revealed that transgene expression occurred mainly in peritubular fibroblasts of the suctioned kidney by tissue clearing and immunohistochemistry. Next, we confirmed the periods of pDNA uptake and activation of transcription factors nuclear factor-jB and activator protein 1 by luciferase assays. Moreover, the use of a pCpG-free plasmid enabled sustained transgene expression in the suctioned kidney. In conclusion, analyses of the spatial distribution and immunostaining of the section suggest that pDNA and transgene expression occurs mainly in peritubular fibroblasts of the suctioned kidney. In addition, we clarified some factors for efficient and/or sustained transgene expression in the suctioned kidney.ARTICLE HISTORY

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Micro-MRI methods to detect renal cysts in mice

Abstract: Micro-MRI with 3D-FIESTA can depict cyst formation in the diseased kidneys of living mice without injection of contrast agents.

Cited by 26 publications

References 14 publications

Imaging Acute Renal Failure with Polyamine Dendrimer-Based MRI Contrast Agents

Imaging Acute Renal Failure with Polyamine Dendrimer-Based MRI Contrast Agents

Monitoring kidney and renal cyst volumes applying MR approaches on a rapamycin treated mouse model of ADPKD

Characterization of transgene expression and pDNA distribution of the suctioned kidney in mice

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