Micro-Optical Laser Induced Fluorescence Detection on a Miniaturised Flow Cytometry Device

Krüger, Jan; Porta, P.; Morrison, Alan P.; Singh, Kamal Deep; O’Neill, Alan P; O'Brien, P.

doi:10.1007/978-94-010-0295-0_97

Cited by 7 publications

(7 citation statements)

References 2 publications

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“…(11) combined with the functions defined in Eqs. (4) and (9); the expected efficiencies can be corrected for viscous drag losses using Eq. (13).…”

Section: Dep Capture Theorymentioning

confidence: 99%

“…However, conventional FACS is expensive, difficult to sterilize, mechanically complex, and requires relatively large sample volumes as well as trained personnel for operation and maintenance. Several groups have developed micro-FACS devices to realize the cell sorting process on a microchip [3][4][5][6][7]. However, for most of these micro-FACS the sorting throughputs were very limited in comparison to conventional FACS.…”

Section: Introductionmentioning

confidence: 99%

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Dielectrophoresis microsystem with integrated flow cytometers for on‐line monitoring of sorting efficiency

et al. 2006

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Dielectrophoresis (DEP) and flow cytometry are powerful technologies and widely applied in microfluidic systems for handling and measuring cells and particles. Here, we present a novel microchip with a DEP selective filter integrated with two microchip flow cytometers (FCs) for on-line monitoring of cell sorting processes. On the microchip, the DEP filter is integrated in a microfluidic channel network to sort yeast cells by positive DEP. The two FCs detection windows are set upstream and downstream of the DEP filter. When a cell passes through the detection windows, the light scattered by the cell is measured by integrated polymer optical elements (waveguide, lens, and fiber coupler). By comparing the cell counting rates measured by the two FCs, the collection efficiency of the DEP filter can be determined. The chips were used for quantitative determination of the effect of flow rate, applied voltage, conductivity of the sample, and frequency of the electric field on the sorting efficiency. A theoretical model for the capture efficiency was developed and a reasonable agreement with the experimental results observed. Viable and non-viable yeast cells showed different frequency dependencies and were sorted with high efficiency. At 2 MHz, more than 90% of the viable and less than 10% of the non-viable cells were captured on the DEP filter. The presented approach provides quantitative real-time data for sorting a large number of cells and will allow optimization of the conditions for, e.g., collecting cancer cells on a DEP filter while normal cells pass through the system. Furthermore, the microstructure is simple to fabricate and can easily be integrated with other microstructures for lab-on-a-chip applications.

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“…(11) combined with the functions defined in Eqs. (4) and (9); the expected efficiencies can be corrected for viscous drag losses using Eq. (13).…”

Section: Dep Capture Theorymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dielectrophoresis microsystem with integrated flow cytometers for on‐line monitoring of sorting efficiency

et al. 2006

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“…A micro-cytometry system for human pathogen exposure to monitor leukocyte populations has been presented [256]. A flow cytometric detection device on-chip has been demonstrated as a novel approach, based on a laser-induced fluorescence detection system, combining solid state laser detection with microfluidic technology [257].…”

Section: Flow Cytometry On Microfluidic Chipmentioning

confidence: 99%

Polymer-Based Microfluidic Devices for Pharmacy, Biology and Tissue Engineering

2012

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This paper reviews microfluidic technologies with emphasis on applications in the fields of pharmacy, biology, and tissue engineering. Design and fabrication of microfluidic systems are discussed with respect to specific biological concerns, such as biocompatibility and cell viability. Recent applications and developments on genetic analysis, cell culture, cell manipulation, biosensors, pathogen detection systems, diagnostic devices, high-throughput screening and biomaterial synthesis for tissue engineering are presented. The pros and cons of materials like polydimethylsiloxane (PDMS), polymethylmethacrylate (PMMA), polystyrene (PS), polycarbonate (PC), cyclic olefin copolymer (COC), glass, and silicon are discussed in terms of biocompatibility and fabrication aspects. Microfluidic devices are widely used in life sciences. Here, commercialization and research trends of microfluidics as new, easy to use, and cost-effective measurement tools at the cell/tissue level are critically reviewed.

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“…A series of still images taken from a device undergoing hydrodynamic switching [15] is shown in figure 7. The device incorporates an 'X'-shaped channel intersection joining input, switch, collection and waste channels.…”

Section: Hydrodynamic Switchingmentioning

confidence: 99%

Development of a microfluidic device for fluorescence activated cell sorting

Krüger¹,

Singh²,

O’Neill³

et al. 2002

J. Micromech. Microeng.

273

121

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This paper describes the development towards a miniaturized analytical system that can perform the major key functions of a flow cytometer. The development aims at diagnostic applications for cell counting and sorting with the ultimate goal of a low-cost portable instrument for point of care diagnosis. The present systems configuration consists of a disposable microfluidic device, that enables injection, single file cell flow through a miniaturized laser induced fluorescence detection system as well as sorting of identified samples. The microfluidic devices were fabricated by means of rapid prototyping technologies based on thick film photo-polymers. This paper reports various approaches on cell sorting and demonstrates sorting of single cells by means of an off-chip valve switching technique. The miniaturized fluorescence detection system employs active and passive micro-optical components, including semiconductor laser and ultra bright LED sources, highly sensitive avalanche photodiodes as well as micro-prism, holographic diffraction gratings and fibre optics for transmission and collection of light. Furthermore we demonstrate the feasibility of integrating solid-state components as part of an on-chip detection system.

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Micro-Optical Laser Induced Fluorescence Detection on a Miniaturised Flow Cytometry Device

Cited by 7 publications

References 2 publications

Dielectrophoresis microsystem with integrated flow cytometers for on‐line monitoring of sorting efficiency

Dielectrophoresis microsystem with integrated flow cytometers for on‐line monitoring of sorting efficiency

Polymer-Based Microfluidic Devices for Pharmacy, Biology and Tissue Engineering

Development of a microfluidic device for fluorescence activated cell sorting

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