Micro<scp>RNA</scp> 200a inhibits erythroid differentiation by targeting <i><scp>PDCD</scp>4</i> and <i><scp>THRB</scp></i>

Liu, Yanming; Zhang, Qian; Du, Zhenglin; Lu, Zhichao; Liu, Shuge; Zhang, Lu; Ding, Nan; Bao, Binghao; Yang, Yadong; Xiong, Qian; Wang, Hai; Zhang, Zhaojun; Qu, Hongzhu; Jia, Haibo; Fang, Xiangdong

doi:10.1111/bjh.14377

Cited by 13 publications

(10 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PDCD4 negatively regulates the translation of several oncogenes such as Cyclins , B-Myb and c-Myb [58,59]. Because of its critical role in several vital biological processes, its cellular level under normal physiological conditions is tightly regulated via several transcriptional and post-transcriptional regulatory mechanisms [60,61,62,63,64,65,66]. Our studies, demonstrating the role of PDCD4-AS1 in enhancing the cellular levels of PDCD4 adds another layer of complexity in PDCD4 regulation during BC progression.…”

Section: Discussionmentioning

confidence: 99%

A natural antisense lncRNA controls breast cancer progression by promoting tumor suppressor gene mRNA stability

et al. 2018

View full text Add to dashboard Cite

The human genome encodes thousands of long noncoding RNA (lncRNA) genes; the function of majority of them is poorly understood. Aberrant expression of a significant number of lncRNAs is observed in various diseases, including cancer. To gain insights into the role of lncRNAs in breast cancer progression, we performed genome-wide transcriptome analyses in an isogenic, triple negative breast cancer (TNBC/basal-like) progression cell lines using a 3D cell culture model. We identified significantly altered expression of 1853 lncRNAs, including ~500 natural antisense transcript (NATs) lncRNAs. A significant number of breast cancer-deregulated NATs displayed co-regulated expression with oncogenic and tumor suppressor protein-coding genes in cis. Further studies on one such NAT, PDCD4-AS1 lncRNA reveal that it positively regulates the expression and activity of the tumor suppressor PDCD4 in mammary epithelial cells. Both PDCD4-AS1 and PDCD4 show reduced expression in TNBC cell lines and in patients, and depletion of PDCD4-AS1 compromised the cellular levels and activity of PDCD4. Further, tumorigenic properties of PDCD4-AS1-depleted TNBC cells were rescued by exogenous expression of PDCD4, implying that PDCD4-AS1 acts upstream of PDCD4. Mechanistically, PDCD4-AS1 stabilizes PDCD4 RNA by forming RNA duplex and controls the interaction between PDCD4 RNA and RNA decay promoting factors such as HuR. Our studies demonstrate crucial roles played by NAT lncRNAs in regulating post-transcriptional gene expression of key oncogenic or tumor suppressor genes, thereby contributing to TNBC progression.

show abstract

Section: Discussionmentioning

confidence: 99%

A natural antisense lncRNA controls breast cancer progression by promoting tumor suppressor gene mRNA stability

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Therefore, the regulatory mechanisms of erythropoiesis in high-altitude environments need to be investigated thoroughly. Sufficient evidence proves that multiple miRNAs play key roles in the process of erythropoiesis (Felli et al, 2005 ; Sun et al, 2015 ; Li et al, 2017 ); thus, we sought to determine whether abundant RBC-derived miRNAs are involved in the regulation of erythropoiesis via transport pathways in high-altitude environments.…”

Section: Discussionmentioning

confidence: 99%

Different Erythrocyte MicroRNA Profiles in Low- and High-Altitude Individuals

Sun

Fan

et al. 2018

Front. Physiol.

View full text Add to dashboard Cite

Background: The number of red blood cells (RBCs) increases significantly in response to high-altitude hypoxic environments, and the RBC microRNA (miRNA) expression pattern is similar to that in whole blood. Studies have shown that miRNA in plasma can act as a circulating hypoxia-associated marker, but the effect of a high-altitude hypoxic environment on RBC-derived miRNAs has not yet been reported.Methods: Blood samples were collected from 20 Han Chinese individuals residing at 500 m (Sichuan Han), 10 migrant Han Chinese citizens residing at 3,658 m (Tibet Han) and 12 native Tibetans, and RBC indices measurements and miRNA sequencing analyses were performed for the three sample groups. The levels of some markedly altered miRNAs at high altitude were subsequently measured from 5 randomly selected samples of each group by real-time PCR. Bioinformatic analyses was performed to determine the potential target genes of selected hypoxia-associated miRNAs.Results: Marked changes of several RBC indices were observed among the Tibet Han population, the Tibetan population and the Sichuan Han population. A total of 516 miRNAs derived from RBCs were initially identified by miRNA sequencing in the three sample groups. Compared with the Sichuan Han population, 49 miRNAs were differentially expressed in the Tibet Han population (17 upregulated and 32 downregulated). 12 upregulated and 21 downregulated miRNAs were observed in the Tibetan population compared with the Sichuan Han population. A total of 40 RBC miRNAs were differentially expressed in the Tibetan population (15 upregulated and 25 downregulated) compared with the Tibet Han population. Two significantly altered miRNAs with the highest expression levels (miRNA-144-5p and miR-30b-5p) were selected for real-time PCR analysis, and the results were consistent with those of miRNA sequencing. Furthermore, bioinformatic analyses showed that some potential target genes of miR-144-5p and miR-30b-5p are involved in the erythroid- hypoxia-, and nitric oxide (NO)-related signaling pathways in response to hypoxia.Conclusion: Our findings provide clear evidence, for the first time, that a high-altitude hypoxic environment significantly affects human RBC miRNA profiles.

show abstract

“…4 ). In particular, increased expression levels of miRNA-200a, -218, -221, -222, -223, 9, -15a, and -320 block, while miRNA-27a, -451, -144, -486-3p, and -146b promote erythropoiesis [ 32 - 46 ]. In addition, decreased expression levels of miRNA-150 promote erythropoiesis [ 39 ].…”

Section: Micrornas In Normal Hematopoiesismentioning

confidence: 99%

MicroRNAs in the Myelodysplastic Syndrome

et al. 2021

View full text Add to dashboard Cite

The myelodysplastic syndrome (MDS) holds a special place among blood cancers, as it represents a whole spectrum of hematological disorders with impaired differentiation of hematopoietic precursors, bone marrow dysplasia, genetic instability and is noted for an increased risk of acute myeloid leukemia. Both genetic and epigenetic factors, including microRNAs (miRNAs), are involved in MDS development. MicroRNAs are short non-coding RNAs that are important regulators of normal hematopoiesis, and abnormal changes in their expression levels can contribute to hematological tumor development. To assess the prognosis of the disease, an international assessment system taking into account a karyotype, the number of blast cells, and the degree of deficiency of different blood cell types is used. However, the overall survival and effectiveness of the therapy offered are not always consistent with predictions. The search for new biomarkers, followed by their integration into the existing prognostic system, will allow for personalized treatment to be performed with more precision. Additionally, this paper explains how miRNA expression levels correlate with the prognosis of overall survival and response to the therapy offered.

show abstract

MicroRNA 200a inhibits erythroid differentiation by targeting PDCD4 and THRB

Cited by 13 publications

References 45 publications

A natural antisense lncRNA controls breast cancer progression by promoting tumor suppressor gene mRNA stability

A natural antisense lncRNA controls breast cancer progression by promoting tumor suppressor gene mRNA stability

Different Erythrocyte MicroRNA Profiles in Low- and High-Altitude Individuals

MicroRNAs in the Myelodysplastic Syndrome

Contact Info

Product

Resources

About