Micro<scp>RNA</scp>‐let‐7c suppresses hepatitis C virus replication by targeting Bach1 for induction of haem oxygenase‐1 expression

Chen, Wei‐Chun; Wei, Chih-Ku; Lee, Jin‐Ching

doi:10.1111/jvh.13072

Cited by 19 publications

(15 citation statements)

References 67 publications

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“…Another targeting miRNA identified in this study, hsa-let-7c-5p, directly affects ACE2 and TMPRSS2; two key players in the SARS-CoV-2 infection ( Chauhan et al, 2020 ).In rhabdomyosarcoma cells, hsa-let-7c-5p promoted the replication of enterovirus 71 (EV71) by inhibiting the MAPK4K expression ( Zhou et al, 2017 ). Overexpression of miR-let-7c attenuated the replication of HCV through HO-1 induction ( Chen et al, 2019 ). A differential expression of hsa-let-7 in-silico as a CRIEG indicates it has a role in immunomodulation in COVID-19.…”

Section: Discussionmentioning

confidence: 99%

MicroRNAs based regulation of cytokine regulating immune expressed genes and their transcription factors in COVID-19

2022

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Background Coronavirus disease 2019 is characterized by the elevation of a broad spectrum of inflammatory mediators associated with poor disease outcomes. We aimed at an in-silico analysis of regulatory microRNA and their transcription factors (TF) for these inflammatory genes that may help to devise potential therapeutic strategies in the future. Methods The cytokine regulating immune-expressed genes (CRIEG) were sorted from literature and the GEO microarray dataset. Their co-differentially expressed miRNA and transcription factors were predicted from publicly available databases. Enrichment analysis was done through mienturnet, MiEAA, Gene Ontology, and pathways predicted by KEGG and Reactome pathways. Finally, the functional and regulatory features were analyzed and visualized through Cytoscape. Results Sixteen CRIEG were observed to have a significant protein-protein interaction network. The ontological analysis revealed significantly enriched pathways for biological processes, molecular functions, and cellular components. The search performed in the miRNA database yielded ten miRNAs that are significantly involved in regulating these genes and their transcription factors. Conclusion An in-silico representation of a network involving miRNAs, CRIEGs, and TF, which take part in the inflammatory response in COVID-19, has been elucidated. Thus, these regulatory factors may have potentially critical roles in the inflammatory response in COVID-19 and may be explored further to develop targeted therapeutic strategies and mechanistic validation.

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Section: Discussionmentioning

confidence: 99%

MicroRNAs based regulation of cytokine regulating immune expressed genes and their transcription factors in COVID-19

2022

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“…The pHO‐1‐Luc reporter vector with the HO‐1 promoter was kindly provided by Dr Anupam Agarwal (University of Alabama, USA) . pCMV‐Bach1‐HA containing the human Bach1 coding region was amplified from pBluescriptR‐Bach1 (BC063307) (GenDiscovery Biotechnology, Inc Taipei, Taiwan) and cloned into the pcDNA3.1 plasmid with an HA tag …”

Section: Methodsmentioning

confidence: 99%

“…29 pCMV-Bach1-HA containing the human Bach1 coding region was amplified from pBluescriptR-Bach1 (BC063307) (GenDiscovery Biotechnology, Inc Taipei, Taiwan) and cloned into the pcDNA3.1 plasmid with an HA tag. 30 2. 4 | Quantification of cellular microRNA,…”

Section: Plasmid Constructionmentioning

confidence: 99%

“…The Western blotting was performed as previously described. 28,30 The membrane was probed with anti-DENV NS2B (1:3000; GeneTex, Inc, Irvine, CA), anti-HO1 (1:3000; Abcam, Cambridge, MA), anti-Bach1 (1:1000; Abcam), anti-keap1 (1:2000; Abcam), anti-Nrf2 (1:1000; GeneTex), anti-HA (1:10 000; GeneTex), and anti-GAPDH antibody (1:10 000; GeneTex).…”

Section: Western Blottingmentioning

confidence: 99%

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MicroRNA‐155 inhibits dengue virus replication by inducing heme oxygenase‐1‐mediated antiviral interferon responses

Huang

et al. 2020

FASEB j.

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MicroRNAs (miRNAs) have been reported to directly alter the virus life cycle and virus–host interactions, and so are considered promising molecules for controlling virus infection. In the present study, we observed that miR‐155 time‐dependently downregulated upon dengue virus (DENV) infection. In contrast, exogenous overexpression of miR‐155 appeared to limit viral replication in vitro, suggesting that the low levels of miR‐155 would be beneficial for DENV replication. In vivo, overexpression of miR‐155 protected ICR suckling mice from the life‐threatening effects of DENV infection and reduced virus propagation. Further investigation revealed that the anti‐DENV activity of miR‐155 was due to target Bach1, resulting in the induction of the heme oxygenase‐1 (HO‐1)‐mediated inhibition of DENV NS2B/NS3 protease activity, ultimately leading to induction of antiviral interferon responses, including interferon‐induced protein kinase R (PKR), 2′‐5′‐oligoadenylate synthetase 1 (OAS1), OAS2, and OAS3 expression, against DENV replication. Collectively, our results provide a promising new strategy to manage DENV infection by modulation of miR‐155 expression.

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“…2017). Overexpression of miR-let-7c attenuated the replication of HCV through HO-1 induction (Chen et al . 2019).…”

Section: Discussionmentioning

confidence: 99%

Micro RNA-based regulation of genomics and transcriptomics of inflammatory cytokines in COVID-19

Khokhar

Tomo

Purohit

2021

Preprint

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Background: Coronavirus disease 2019 is characterized by the elevation of a wide spectrum of inflammatory mediators which are associated with poor disease outcomes. We aimed at an in-silico analysis of regulatory microRNA and their transcription factors (TF) for these inflammatory genes that may help to devise potential therapeutic strategies in the future. Methods: The cytokine regulating immune-expressed genes (CRIEG) were sorted from literature and GEO microarray dataset and their co-differentially expressed miRNA and transcription factors were predicted from publicly available databases. Enrichment analysis was done through mienturnet, MiEAA, and Gene Ontology, and pathways predicted by KEGG and Reactome pathways. The functional and regulatory features were analyzed and visualized through Cytoscape. Results: Sixteen CRIEG were observed to have a significant protein-protein interaction network. The ontological analysis revealed significantly enriched pathways for biological processes, molecular functions, and cellular components. The search performed in the miRNA database yielded 10 miRNAs that are significantly involved in the regulation of these genes and their transcription factors. Conclusion: An In-Silico representation of a network involving miRNAs, CRIEGs, and TF which take part in the inflammatory response in COVID-19 has been elucidated. These regulatory factors may have potentially critical roles in the inflammatory response in COVID-19 and may be explored further for the development of targeted therapeutic strategies and mechanistic validation.

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MicroRNA‐let‐7c suppresses hepatitis C virus replication by targeting Bach1 for induction of haem oxygenase‐1 expression

Cited by 19 publications

References 67 publications

MicroRNAs based regulation of cytokine regulating immune expressed genes and their transcription factors in COVID-19

MicroRNAs based regulation of cytokine regulating immune expressed genes and their transcription factors in COVID-19

MicroRNA‐155 inhibits dengue virus replication by inducing heme oxygenase‐1‐mediated antiviral interferon responses

Micro RNA-based regulation of genomics and transcriptomics of inflammatory cytokines in COVID-19

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