Micro
            <scp>RNAs</scp>
            in Development and Disease

Staton, Alison A; Giráldez, Antonio J.

doi:10.1002/9780470015902.a0020863

Cited by 3 publications

(4 citation statements)

References 86 publications

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“…While early studies have emphasized the role of miRNAs during differentiation (Chen et al 2006), miRNAs as a class are expressed throughout the life of the organism, implying that members of this RNA class regulate specific cellular properties and tissue physiology (Poy et al 2004;Krutzfeldt et al 2005;Staton and Giraldez 2008;van Rooij et al 2008). At present we cannot exclude the possibility that the phenotype observed might be due to the combined misregulation of targets affecting different biological processes.…”

Section: Discussionmentioning

confidence: 99%

“…microRNAs (miRNAs) are ;22-nucleotide (nt) RNAs that control gene expression at the post-transcriptional level through various regulatory mechanisms, including messenger RNA (mRNA) deadenylation, translation, and decay of target mRNAs (Bushati and Cohen 2007;Filipowicz et al 2008;Staton and Giraldez 2008). miRNAs play key roles in animal development where they regulate multiple cellular processes including cell fate specification, cell signaling, and tissue morphogenesis (Kloosterman and Plasterk 2006;Staton and Giraldez 2008).…”

mentioning

confidence: 99%

“…miRNAs play key roles in animal development where they regulate multiple cellular processes including cell fate specification, cell signaling, and tissue morphogenesis (Kloosterman and Plasterk 2006;Staton and Giraldez 2008). Despite the recent progress in defining the phenotypic functions of specific miRNAs, the mechanisms by which miRNAs regulate gene expression during different developmental processes are still poorly understood.…”

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confidence: 99%

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Zebrafish miR-1 and miR-133 shape muscle gene expression and regulate sarcomeric actin organization

Mishima

Abreu‐Goodger²,

Staton³

et al. 2009

Genes Dev.

152

142

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microRNAs (miRNAs) represent ;4% of the genes in vertebrates, where they regulate deadenylation, translation, and decay of the target messenger RNAs (mRNAs). The integrated role of miRNAs to regulate gene expression and cell function remains largely unknown. Therefore, to identify the targets coordinately regulated by muscle miRNAs in vivo, we performed gene expression arrays on muscle cells sorted from wild type, dicer mutants, and single miRNA knockdown embryos. Our analysis reveals that two particular miRNAs, miR-1 and miR-133, influence gene expression patterns in the zebrafish embryo where they account for >54% of the miRNA-mediated regulation in the muscle. We also found that muscle miRNA targets (1) tend to be expressed at low levels in wildtype muscle but are more highly expressed in dicer mutant muscle, and (2) are enriched for actin-related and actinbinding proteins. Loss of dicer function or down-regulation of miR-1 and miR-133 alters muscle gene expression and disrupts actin organization during sarcomere assembly. These results suggest that miR-1 and miR-133 actively shape gene expression patterns in muscle tissue, where they regulate sarcomeric actin organization.[Keywords: microRNAs; muscle; target identification; zebrafish] Supplemental material is available at http://www.genesdev.org.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Zebrafish miR-1 and miR-133 shape muscle gene expression and regulate sarcomeric actin organization

Mishima

Abreu‐Goodger²,

Staton³

et al. 2009

Genes Dev.

152

142

View full text Add to dashboard Cite

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“…Developmental, homeostatic, and disease processes are modulated by miRNA-mediated repression of transcripts 2, 3 . This selective repression by miRNAs is generally conferred by bases 2-8 of the mature miRNA.…”

Section: Introductionmentioning

confidence: 99%

Use of target protector morpholinos to analyze the physiological roles of specific miRNA-mRNA pairs in vivo

Staton

Giráldez

2011

Nat Protoc

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MicroRNAs (miRNAs) regulate gene expression by pairing to complementary sequences in the 3’UTRs of transcripts. Though the molecular mechanism underlying miRNA biogenesis and activity is becoming better understood, determining the physiological role of the interaction of a miRNA with its target remains a challenge. A number of methods have been developed to inhibit individual miRNAs, but it can be difficult to determine which specific targets are responsible for any observed phenotypes. To address this problem, we use Target Protector morpholinos interfere with a single miRNA-mRNA pair by binding specifically to the miRNA target sequence in the 3’UTR. In this protocol, we detail the steps for identifying miRNA targets, validating their regulation, and using target protectors to interrogate their function in zebrafish. Depending on the biological context, this set of experiments has been completed in 6-8 weeks.

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MicroRNA s in Cardiovascular Development and Diseases

Mishra

Yadav

Rani

et al. 2015

Encyclopedia of Life Sciences

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Small molecule microribonucleic acids (miRNAs) are coming up with a huge impact on the scientific world of therapeutics and diagnostics. These 22–24 nucleotide small noncoding RNAs acting as negative gene regulators are found to be involved in a broad range of homeostasis process. One of the major health‐related concerns in current scenario is the development of heart problems leading to cardiomyopathy and heart failure in extreme conditions. The disease condition is associated with both lifestyle and genetic factors. The role of miRNAs governing the regulation of the genes involved in heart development and diseases is being studied extensively. Some of the miRNAs present abundantly in heart and regulating the process of cardiovascular development and diseases are miR‐1, miR‐133, miR‐499, miR‐208 and miR‐199b. This article presents a brief review on the roles of these miRNAs in cardiac development and diseases along with their potential in heart regeneration. Key Concepts A large fraction of miRNAs are expressed in heart regulating the gene expression. miR‐1/133b, miR‐208/499 and miR‐17‐92 clusters are majorly involved in the cardiovascular systems. An imbalance or downregulation of the crucial miRNAs is associated with the development of cardiac diseases. Heart regeneration can be achieved by the exogenous supply of deregulated miRNAs.

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Micro RNAs in Development and Disease

Cited by 3 publications

References 86 publications

Zebrafish miR-1 and miR-133 shape muscle gene expression and regulate sarcomeric actin organization

Zebrafish miR-1 and miR-133 shape muscle gene expression and regulate sarcomeric actin organization

Use of target protector morpholinos to analyze the physiological roles of specific miRNA-mRNA pairs in vivo

MicroRNA s in Cardiovascular Development and Diseases

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