Microalgae as an Efficient Vehicle for the Production and Targeted Delivery of Therapeutic Glycoproteins against SARS-CoV-2 Variants

Dehghani, Jaber; Movafeghi, Ali; Mathieu‐Rivet, Elodie; Mati‐Baouche, Narimane; Calbo, Sébastien; Lerouge, Patrice; Bardor, Muriel

doi:10.3390/md20110657

Cited by 16 publications

(9 citation statements)

References 138 publications

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“…There is significant potential for microalgae to be engineered for improved nutritional value and to serve as novel therapeutic production platforms. − The nutritional value of alga strains could conceivably be augmented via bioengineering to produce honey bee nutrient storage proteins, such as vitellogenin and royal jelly proteins. Such alga strains could more closely recapitulate the dietary amino acid requirements of bees.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of a Microalgal Feed Additive in Commercial Honey Bee Colonies Used for Crop Pollination

McMenamin,

Weiss,

Meikle

et al. 2023

ACS Agric. Sci. Technol.

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Honey bees are experiencing nutritional deficiencies related to intensive agriculture and climate change, posing major threats to the ecosystem services they provide. Colonies managed for crop pollination are fed artificial diets to offset reduced pollen in the environment and to stimulate population growth. Here, we evaluated the effects of a spirulina microalga feed additive on commercial honey bee colonies in an agriculturally intensive desert environment. One hundred honey bee colonies were used in a randomized block design that was replicated at two apiary sites over 4 months leading up to spring almond pollination. Colonies were fed a pollen-free artificial diet or the same diet containing 25% spirulina. Unfed colonies were randomly assigned within treatment blocks as controls. We measured colony population size, brood production, thermoregulation, and a panel of molecular biomarkers associated with nutritional status, stress responses, and gut microbiota. Hive brood imaging and continuous temperature data enabled sensitive detection of diet treatment effects. Spirulina feed significantly improved brood production and thermoregulation prior to almond pollination relative to unfed controls. Bees fed spirulina had distinct expression profiles of nutrition and stress response genes, but gene expression was primarily driven by the apiary site. We conclude that spirulina is a sustainable feed additive with potential to improve crop pollination efficiency by supporting larger, healthier honey bee colonies.

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Section: Discussionmentioning

confidence: 99%

Efficacy of a Microalgal Feed Additive in Commercial Honey Bee Colonies Used for Crop Pollination

McMenamin,

Weiss,

Meikle

et al. 2023

ACS Agric. Sci. Technol.

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“…Chlamydomonas has also emerged as an organism with great biotechnological potential [ 34 ]. It is worth noting its use for the production of vaccines [ 35 ], antibodies [ 36 ], or in human clinical trials aimed to improve gastrointestinal health [ 37 ]. Furthermore, Chlamydomonas is also an excellent model for studying the basis of mutualistic interactions with bacteria, based on carbon–nitrogen exchange [ 38 ].…”

Section: Chlamydomonas Reinhardtii As a Model In Mo Homeostasismentioning

confidence: 99%

Chlamydomonas reinhardtii—A Reference Microorganism for Eukaryotic Molybdenum Metabolism

Tejada-Jimenez,

Leon-Miranda,

Llamas

2023

Microorganisms

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Molybdenum (Mo) is vital for the activity of a small but essential group of enzymes called molybdoenzymes. So far, specifically five molybdoenzymes have been discovered in eukaryotes: nitrate reductase, sulfite oxidase, xanthine dehydrogenase, aldehyde oxidase, and mARC. In order to become biologically active, Mo must be chelated to a pterin, forming the so-called Mo cofactor (Moco). Deficiency or mutation in any of the genes involved in Moco biosynthesis results in the simultaneous loss of activity of all molybdoenzymes, fully or partially preventing the normal development of the affected organism. To prevent this, the different mechanisms involved in Mo homeostasis must be finely regulated. Chlamydomonas reinhardtii is a unicellular, photosynthetic, eukaryotic microalga that has produced fundamental advances in key steps of Mo homeostasis over the last 30 years, which have been extrapolated to higher organisms, both plants and animals. These advances include the identification of the first two molybdate transporters in eukaryotes (MOT1 and MOT2), the characterization of key genes in Moco biosynthesis, the identification of the first enzyme that protects and transfers Moco (MCP1), the first characterization of mARC in plants, and the discovery of the crucial role of the nitrate reductase–mARC complex in plant nitric oxide production. This review aims to provide a comprehensive summary of the progress achieved in using C. reinhardtii as a model organism in Mo homeostasis and to propose how this microalga can continue improving with the advancements in this field in the future.

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“…[ 16–20 ] Gene engineered microalgae have been tested in protein drug development [ 21 ] and edible vaccine production. [ 22–24 ]…”

Section: Introductionmentioning

confidence: 99%

Recent advances and fundamentals of microalgae cultivation technology

Rozenberg,

Sorokin,

Mukhambetova

et al. 2024

Biotechnology Journal

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Microalgae are considered to be a promising group of organisms for fuel production, waste processing, pharmaceutical applications, and as a source of food components. Unicellular algae are worth being considered because of their capacity to produce comparatively large amounts of lipids, proteins, and vitamins while requiring little room for growth. They can also grow on waste and fix CO2 and nitrogen compounds. However, production costs limit the industrial use of microalgae to the most profitable applications including micronutrient production and fish farming. Therefore, novel microalgae based technologies require an increase of the production efficiencies or values. Here we review the recent studies focused on getting strains with novel characteristics or cultivating techniques that improve production's robustness or efficiency and categorize these findings according to the fundamental factors that determine microalgae growth. Improvements of light and nutrient delivery, as well as other aspects of photobioreactor design, have shown the highest average increase in productivity. Other methods, such as an improvement of phosphorus or CO2 fixation and temperature adaptation have been found to be less effective. Furthermore, interactions with particular bacteria may promote the growth of microalgae, although bacterial and grazer contaminations must be managed to avoid culture failure. The competitiveness of the algal products will increase if these discoveries are applied to industrial settings.

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Microalgae as an Efficient Vehicle for the Production and Targeted Delivery of Therapeutic Glycoproteins against SARS-CoV-2 Variants

Cited by 16 publications

References 138 publications

Efficacy of a Microalgal Feed Additive in Commercial Honey Bee Colonies Used for Crop Pollination

Efficacy of a Microalgal Feed Additive in Commercial Honey Bee Colonies Used for Crop Pollination

Chlamydomonas reinhardtii—A Reference Microorganism for Eukaryotic Molybdenum Metabolism

Recent advances and fundamentals of microalgae cultivation technology

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