Microalgae dual-head biosensors for selective detection of herbicides with fiber-optic luminescent O2 transduction

Haigh-Flórez, David; Hera, Cristina de la; Costas, Eduardo; Orellana, Guillermo

doi:10.1016/j.bios.2013.10.062

Cited by 36 publications

(15 citation statements)

References 41 publications

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“…Manufacturing of the former, to final dimensions of 4 mm (dia.) and 250 μm thickness (including an opacifying layer made of Dow Corning 3145G grey silicone), has been reported [31]. In brief, DC3140 silicone and pyrogenic silica (10% w/w) were blended, knife-spread as a thin film (125 μm) and allowed to cure at 70 ºC for 72 h. Then, the film was briefly soaked in dichloromethane containing 0.25 mmol L -1 of RD3 indicator, the dyed film was rinsed with dichloromethane and the residual solvent allowed to evaporate for 48 h. Then, the O 2 -sensing film was coated on one side with a ca.…”

Section: 3mentioning

confidence: 99%

Rugged fibre-optic luminescent sensor for CO2 determination in microalgae photoreactors for biofuel production

Haigh-Flórez¹,

Cano-Raya²,

Bedoya³

et al. 2015

Sensors and Actuators B: Chemical

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Section: 3mentioning

confidence: 99%

Rugged fibre-optic luminescent sensor for CO2 determination in microalgae photoreactors for biofuel production

Haigh-Flórez¹,

Cano-Raya²,

Bedoya³

et al. 2015

Sensors and Actuators B: Chemical

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“…Thus, national and international regulations are encouraging minimizing or banning the use of pesticides. For an example, the European Union (EU), through their Water Framework Directive (WFD, 2000/60/EC) or the Urban Waste Water Directive (91/271/EEC), has strengthened water quality control and set a drinking water threshold of 0.1 µg/L for each individual pesticide and 0.5 µg /L for the sum of all pesticides [13,14].…”

Section: Introductionmentioning

confidence: 99%

Development of Microalgae Biosensor Chip by Incorporating Microarray Oxygen Sensor for Pesticides Sensing

et al. 2019

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A microalgae (Pseudokirchneriella subcapitata) biosensor chip for pesticide sensing has been developed by attaching the immobilized microalgae biofilm pon the microarray dye spots (size 100 μm and pitch 200 μm). The dye spots (ruthenium complex) were printed upon SO3-modified glass slides using a polydimethylsiloxane (PDMS) stamp and a microcontact printer (μCP). Emitted fluorescence intensity (FI) variance due to photosynthetic activity (O2 production) of microalgae was monitored by an inverted fluorescent microscope and inhibition of the oxygen generation rate was calculated based on the FI responses both before and after injection of pesticide sample. The calibration curves, as the inhibition of oxygen generation rate (%) due to photosynthetic activity inhibition by the pesticides, depicted that among the 6 tested pesticides, the biosensor showed good sensitivity for 4 pesticides (diuron, simetryn, simazine, and atrazine) but was insensitive for mefenacet and pendimethalin. The detection limits were 1 ppb for diuron and 10 ppb for simetryn, simazine, and atrazine. The simple and low-cost nature of sensing of the developed biosensor sensor chip has apparently created opportunities for regular water quality monitoring, where pesticides are an important concern.

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“…It is demonstrated in literature that fluorescence-based biosensors employed for the detection of Diuron have often high performances with low limits of detection (Naessens et al, 2000). However, they often demand high stabilization times and can only be effective when optically clear, not turbid samples are examined (Haigh-Flórez et al, 2014). Consequently, a complementary electrochemical biosensor can be beneficial to the determination of pollution level as this type of sensor can yield solid and stable systems that are easily miniaturized and simple to use.…”

Section: Introductionmentioning

confidence: 99%

Development of a lab-on-chip electrochemical biosensor for water quality analysis based on microalgal photosynthesis

Tsopela

Laborde

Salvagnac

et al. 2016

Biosensors and Bioelectronics

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The present work was dedicated to the development of a lab-on-chip device for water toxicity analysis and more particularly herbicide detection in water. It consists in a portable system for on-site detection composed of three-electrode electrochemical microcells, integrated on a fluidic platform constructed on a glass substrate. The final goal is to yield a system that gives the possibility of conducting double, complementary detection: electrochemical and optical and therefore all materials used for the fabrication of the lab-on-chip platform were selected in order to obtain a device compatible with optical technology. The basic detection principle consisted in electrochemically monitoring disturbances in metabolic photosynthetic activities of algae induced by the presence of Diuron herbicide. Algal response, evaluated through oxygen (O2) monitoring through photosynthesis was different for each herbicide concentration in the examined sample. A concentration-dependent inhibition effect of the herbicide on photosynthesis was demonstrated. Herbicide detection was achieved through a range (blank - 1 µM Diuron herbicide solution) covering the limit of maximum acceptable concentration imposed by Canadian government (0.64 µM), using a halogen white light source for the stimulation of algal photosynthetic apparatus. Superior sensitivity results (limit of detection of around 0.1 µM) were obtained with an organic light emitting diode (OLED), having an emission spectrum adapted to algal absorption spectrum and assembled on the final system.

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Microalgae dual-head biosensors for selective detection of herbicides with fiber-optic luminescent O2 transduction

Cited by 36 publications

References 41 publications

Rugged fibre-optic luminescent sensor for CO2 determination in microalgae photoreactors for biofuel production

Rugged fibre-optic luminescent sensor for CO2 determination in microalgae photoreactors for biofuel production

Development of Microalgae Biosensor Chip by Incorporating Microarray Oxygen Sensor for Pesticides Sensing

Development of a lab-on-chip electrochemical biosensor for water quality analysis based on microalgal photosynthesis

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