Microalgae screening for heterotrophic and mixotrophic growth on butyrate

Lacroux, Julien; Jouannais, P.; Atteia, Ariane; Bonnafous, Anaïs; Trably, Éric; Steyer, Jean‐Philippe; Lis, Robert van

doi:10.1016/j.algal.2022.102843

Cited by 14 publications

(5 citation statements)

References 62 publications

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“…DNA was extracted using a phenol/chloroform method as in Lacroux et al (2022). In brief, biological matter from pellets and filters was resuspended in a TEN buffer (10 mM Tris pH 8.0, 10 mM EDTA, 150 mM NaCl) and further lysed at 37°C for 30 min in SDS-EB buffer (100 mM Tris pH 8.0, 400 mM NaCl, 40 mM EDTA, 2% SDS) containing RNase A at a final concentration of 40 μg μL −1 .…”

Section: Molecular Analysismentioning

confidence: 99%

Evaluation of sequential filtration and centrifugation to capture environmental DNA and survey microbial eukaryotic communities in aquatic environments

Atteia,

Bec,

Gianaroli

et al. 2023

Molecular Ecology Resources

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Sequential membrane filtration of water samples is commonly used to monitor the diversity of aquatic microbial eukaryotes. This capture method is efficient to focus on specific taxonomic groups within a size fraction, but it is time‐consuming. Centrifugation, often used to collect microorganisms from pure culture, could be seen as an alternative to capture microbial eukaryotic communities from environmental samples. Here, we compared the two capture methods to assess diversity and ecological patterns of eukaryotic communities in the Thau lagoon, France. Water samples were taken twice a month over a full year and sequential filtration targeting the picoplankton (0.2–3 μm) and larger organisms (>3 μm) was used in parallel to centrifugation. The microbial eukaryotic community in the samples was described using an environmental DNA approach targeting the V4 region of the 18S rRNA gene. The most abundant divisions in the filtration fractions and the centrifugation pellet were Dinoflagellata, Metazoa, Ochrophyta, Cryptophyta. Chlorophyta were dominant in the centrifugation pellet and the picoplankton fraction but not in the larger fraction. Diversity indices and structuring patterns of the community in the two size fractions and the centrifugation pellet were comparable. Twenty amplicon sequence variants were significantly differentially abundant between the two size fractions and the centrifugation pellet, and their temporal patterns of abundance in the two fractions combined were similar to those obtained with centrifugation. Overall, centrifugation led to similar ecological conclusions as the two filtrated fractions combined, thus making it an attractive time‐efficient alternative to sequential filtration.

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Section: Molecular Analysismentioning

confidence: 99%

Evaluation of sequential filtration and centrifugation to capture environmental DNA and survey microbial eukaryotic communities in aquatic environments

Atteia,

Bec,

Gianaroli

et al. 2023

Molecular Ecology Resources

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“…Heterotrophic and mixotrophic cultivation approaches have been commonly employed for microalgal biomass production concomitant with carotenoid synthesis [ 126 , 127 ]. The heterotrophic culture system relies on the dark supplementation of diverse carbon sources, including glucose, acetate, and glycerol [ 97 , 108 ].…”

Section: Optimization Of Culture Parameters For Enhancement Of Microa...mentioning

confidence: 99%

Microalgal Carotenoids: Therapeutic Application and Latest Approaches to Enhance the Production

Sirohi

Verma²,

Singh

et al. 2022

CIMB

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Microalgae are microscopic photosynthetic organisms frequently found in fresh and marine water ecosystems. Various microalgal species have been considered a reservoir of diverse health-value products, including vitamins, proteins, lipids, and polysaccharides, and are broadly utilized as food and for the treatment of human ailments such as cancer, cardiovascular diseases, allergies, and immunodeficiency. Microalgae-derived carotenoids are the type of accessory pigment that possess light-absorbing potential and play a significant role in metabolic functions. To date, nearly a thousand carotenoids have been reported, but a very less number of microalgae have been used for the commercial production of carotenoids. This review article briefly discussed the carotenoids of microalgal origin and their therapeutic application. In addition, we have briefly compiled the optimization of culture parameters used to enhance microalgal carotenoid production. In addition, the latest biotechnological approaches used to improve the yields of carotenoid has also been discussed.

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“…Indeed, this species can accumulate up to 50% of its dry weight in lipids, essentially in the form of triacylglycerol (TAG). In addition, its capacity to grow in the main dark fermentation effluents has been demonstrated, under heterotrophic or mixotrophic conditions (Turon et al, 2015a, Lacroux et al, 2022.…”

Section: Introductionmentioning

confidence: 99%

“…Here, we extend further this model by including the consumption of glucose and glycerol by Chlorella and propose a universal multi-substrate dynamic reduced metabolic model. This model is the cornerstone for tackling the major issue due to the high concentration of butyrate which is slowly consumed by microalgae and inhibited by acetate (Hu et al, 2012, Turon et al, 2015b, Lacroux et al, 2022. To address this problem, another organic substrate can be added to lever the inhibition effect of butyrate and eventually accelerate growth in dark fermentation effluents.…”

Section: Introductionmentioning

confidence: 99%

A universal dynamical metabolic model representing mixotrophic growth of Chlorella sp. on wastes

et al. 2023

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Microalgae screening for heterotrophic and mixotrophic growth on butyrate

Cited by 14 publications

References 62 publications

Evaluation of sequential filtration and centrifugation to capture environmental DNA and survey microbial eukaryotic communities in aquatic environments

Evaluation of sequential filtration and centrifugation to capture environmental DNA and survey microbial eukaryotic communities in aquatic environments

Microalgal Carotenoids: Therapeutic Application and Latest Approaches to Enhance the Production

A universal dynamical metabolic model representing mixotrophic growth of Chlorella sp. on wastes

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