Microalgal-bacterial aggregates: Applications and perspectives for wastewater treatment

Quijano, Guillermo; Arcila, Juan S.; Buitrón, Germán

doi:10.1016/j.biotechadv.2017.07.003

Cited by 249 publications

(101 citation statements)

References 71 publications

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“…). This has been reported for other bacterial and cyanobacterial strains in which EPS promote aggregation and embedment of the cells, which may work as a mechanism of self‐shading with the polymers acting as protective molecules against light stress (Miranda et al ., ; Quijano et al ., ). Furthermore, according to Trautmann and colleagues (), this Synechocystis substrain has already the predisposition to exhibit a stress‐induced clumping phenotype due to the truncation of genes encoding the proteins Sll1951 (hemolysin HlyA) and Slr1753 (surface protein), and here, we show that this feature can be exacerbated by the absence of SigF.…”

Section: Discussionmentioning

confidence: 97%

“…It is likely that the light intensity used here is one of the stress factors, as previous studies reported that SigF is crucial for light acclimation responses in Synechocystis (Huckauf et al ., ; Ogawa et al ., ). Furthermore, this hypothesis agrees with the higher amount of RPS and the strong clumping phenotype of the mutant, which are usual responses to light‐induced stress (Miranda et al ., ; Quijano et al ., ). On the other hand, the lower levels/activities of some stress oxidative defences in Δ sigF are either due to regulatory adjustments or to the energetic burden that they represent (Supporting Information Fig.…”

Section: Discussionmentioning

confidence: 97%

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The alternative sigma factor SigF is a key player in the control of secretion mechanisms in Synechocystis sp. PCC 6803

Flores

Santos

Pereira

et al. 2018

Environmental Microbiology

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Summary Cyanobacterial alternative sigma factors are crucial players in environmental adaptation processes, which may involve bacterial responses related to maintenance of cell envelope and control of secretion pathways. Here, we show that the Group 3 alternative sigma factor F (SigF) plays a pleiotropic role in Synechocystis sp. PCC 6803 physiology, with a major impact on growth and secretion mechanisms, such as the production of extracellular polysaccharides, vesiculation and protein secretion. Although ΔsigF growth was significantly impaired, the production of released polysaccharides (RPS) increased threefold to fourfold compared with the wild‐type. ΔsigF exhibits also impairment in formation of outer‐membrane vesicles (OMVs) and pili, as well as several other cell envelope alterations. Similarly, the exoproteome composition of ΔsigF differs from the wild‐type both in amount and type of proteins identified. Quantitative proteomics (iTRAQ) and an in silico analysis of SigF binding motifs revealed possible targets/pathways under SigF control. Besides changes in protein levels involved in secretion mechanisms, our results indicated that photosynthesis, central carbon metabolism and protein folding/degradation mechanisms are altered in ΔsigF. Overall, this work provided new evidences about the role of SigF on Synechocystis physiology and associates this regulatory element with classical and non‐classical secretion pathways.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

The alternative sigma factor SigF is a key player in the control of secretion mechanisms in Synechocystis sp. PCC 6803

Flores

Santos

Pereira

et al. 2018

Environmental Microbiology

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“…Thus, microalgal photosynthesis generates the oxygen (O 2 ) required for chemical oxygen demand (COD) removal. 7 Centrate treatment has been typically investigated in high rate algal ponds (HRAPs) devoted to biogas upgrading. However, the treatment of centrates and other high-strength wastewaters in HRAPs might be limited by the hydraulic retention time (HRT) required for achieving satisfactory COD and nutrients removal.…”

Section: Introductionmentioning

confidence: 99%

Microalgal–bacterial aggregates with flue gas supply as a platform for the treatment of anaerobic digestion centrate

Vital‐Jácome

Díaz‐Zamorano

Cuautle-Marín

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND: Centrate treatment using microalgal-bacterial processes might be limited by the hydraulic retention time (HRT) required to achieve satisfactory chemical oxygen demand (COD) and nutrients removal. Moreover, the poor settling of microalgal biomass still limits the technical and economic performance of microalgal-bacterial processes. In this work, the performance of microalgal-bacterial aggregates (MABAs) supplied with flue gas was investigated as an effective strategy to improve the treatment of centrate from anaerobic digestion of winery wastewater. RESULTS: MABAs supplied with flue gas achieved maximum soluble COD, N-NO 3− , P-PO 4 3− and N-NH 4 + removal efficiencies of 95%, 94%, 100%, and 100%, respectively, in five-fold centrate dilution within 7 days of operation. Centrate turbidity or its components did not hinder the performance of the MABAs under the conditions tested and no aggregates were formed in controls without MABAs inoculation. The mean diameter of the MABAs after centrate treatment was the same or even larger than that of the aggregates of the inoculum. Scanning electron microscopy analyses showed that the liquid medium composition influenced the structure and the type of microalgae cells established in the MABAs. CONCLUSION: MABAs-based centrate treatment supported by flue gas is a promising technology for improving COD and nutrients removal from centrate as well as further biomass harvesting.

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“…Microalgal-based processes have received increasing attention worldwide due to their key characteristics, such as the use of solar light as main energy source and their environmentally friendly nature. 1,2 Nowadays, a sort of processes using microalgal cultures have been developed including biogas upgrading technologies and biodiesel production from microalgal biomass grown with the CO 2 present in flue gases. [3][4][5] At full scale, the efficiency of microalgal-based technologies relies to a large extent on the outdoor conditions and particularly on the solar irradiance.…”

Section: Introductionmentioning

confidence: 99%

“…NOTATION CO 2, Gas CO 2 concentration in gas phase (g m Gas -3 ) CO 2, Liq CO 2 concentration in liquid phase (g m Liq -3 ) CO 2, Tot Total mass of CO 2…”

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confidence: 99%

Kinetic characterization of Scenedesmus quadricauda under low irradiation conditions

Buitrón

Figueroa‐González

Quijano

2017

J of Chemical Tech & Biotech

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BACKGROUND The kinetic parameters of a model culture of Scenedesmus quadricauda were assessed under low light irradiation conditions. A simple and reliable method was developed for the kinetic characterization, which avoided CO2 mass transfer limitations and allowed the retrieval of accurate kinetic data. Biomass and oxygen yields were also assessed. RESULTS A maximum specific CO2 uptake rate of 0.015 ± 0.002 gCO2 g‐1VS h‐1 and half‐saturation constant of 91 ± 13 gCO2 mLiq‐3 were determined for S. quadricauda at a light intensity of 30 µmol m‐2 s‐1 at 25 °C. The biomass and oxygen yields ranged from 0.045–0.137 g VS g‐1CO2 and 0.211–0.505 gO2 g‐1CO2, respectively, both parameters being dependent on the initial CO2 concentration used. CONCLUSION A simple and reliable methodology for the kinetic characterization of microalgal cultures was developed and tested. It was demonstrated that the maximum specific CO2 uptake rate of S. quadricauda at 30 µmol m‐2 s‐1 was higher than previous values reported for Scenedesmus cultures exposed to light intensities up to 4.5 times higher but limited by CO2 mass transfer. The methodology and results obtained are useful for determining whether or not to implement a given microalgal‐based process under low irradiance conditions. © 2017 Society of Chemical Industry

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Microalgal-bacterial aggregates: Applications and perspectives for wastewater treatment

Cited by 249 publications

References 71 publications

The alternative sigma factor SigF is a key player in the control of secretion mechanisms in Synechocystis sp. PCC 6803

The alternative sigma factor SigF is a key player in the control of secretion mechanisms in Synechocystis sp. PCC 6803

Microalgal–bacterial aggregates with flue gas supply as a platform for the treatment of anaerobic digestion centrate

Kinetic characterization of Scenedesmus quadricauda under low irradiation conditions

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