Microarray Analysis and Description of Smr1 Gene in Rat Penis in a Post-Radical Prostatectomy Model of Erectile Dysfunction

User, Herbert M.; Zelner, David J.; McKenna, Kevin E.; McVary, Kevin T.

doi:10.1097/01.ju.0000060882.75475.5a

Cited by 27 publications

(29 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Diabetes is a risk factor for both CVD and ED, and we have previously reported that in diabetic patients and diabetic animal models the genes encoding opiorphin-related products are downregulated in CSM tissue (1,(42)(43)(44)46). In the present study, we demonstrate that experimental diabetes is also associated with reduced Vcsa1 gene expression in other tissues and with significantly lower plasma levels of sialorphin, the rat opiorphin homologue.…”

Section: Discussionsupporting

confidence: 63%

Reversal of diabetic vasculopathy in a rat model of type 1 diabetes by opiorphin-related peptides

Calenda

Tong²,

Kanika³

et al. 2011

American Journal of Physiology-Heart and Circulatory Physiology

View full text Add to dashboard Cite

Diabetes results in a myriad of vascular complications, often referred to as diabetic vasculopathy, which encompasses both microvascular [erectile dysfunction (ED), retinopathy, neuropathy, and nephropathy] and macrovascular complications (hypertension, coronary heart disease, and myocardial infarction). In diabetic animals and patients with ED, there is decreased opiorphin or opiorphin-related gene expression in corporal tissue. Both opiorphin and the rat homologous peptide sialorphin are found circulating in the plasma. In the present study, we investigated if diabetes induced changes in plasma sialorphin levels and if changes in these levels could modulate the biochemistry and physiology of vascular smooth muscle. We show that circulating sialorphin levels are reduced in a rat model of type I diabetes. Intracorporal injection of plasmids expressing sialorphin into diabetic rats restores sialorphin levels to those seen in the blood of nondiabetic animals and results in both improved erectile function and blood pressure. Sialorphin modulated the ability of C-type natriuretic peptide to relax both corporal and aortic smooth muscle strips and of bradykinin to regulate intracellular calcium levels in both corporal and aortic smooth muscle cells. We have previously shown that expression of genes encoding opiorphins is increased when erectile function is improved. Our findings thus suggest that by affecting circulating levels of opiorphin-related peptides, proper erectile function is not only an indicator but also a modulator of overall vascular health of a man.

show abstract

Section: Discussionsupporting

confidence: 63%

Reversal of diabetic vasculopathy in a rat model of type 1 diabetes by opiorphin-related peptides

Calenda

Tong²,

Kanika³

et al. 2011

American Journal of Physiology-Heart and Circulatory Physiology

View full text Add to dashboard Cite

show abstract

“…17 Micro array analysis showed a diversity of RNA expression in penile tissue. 18 Similar protein expression was observed in vascular smooth muscle. 19,20 These results imply that the protein composition in rat penile corpus cavernosum shows to have similar pattern of expression to other tissues 19 and that these proteins may participate in the physiological and pathological functions of penis.…”

Section: Characters Of Protein Expression In Penile Tissuesupporting

confidence: 60%

“…9 It has been reported that CNR induced various responses in penile corpus cavernosum including apoptosis. 18 CNR model showed several changes in functional and molecular aspects, 18 the functional characteristics, however, were not fully defined. In this study, we found that RhoGDI content was increased in penile corpus cavernosum form CNR rats compared with sham-operated rats, which was confirmed with Western blot analysis.…”

Section: Changes Of Rhogdi Protein During Cnrmentioning

confidence: 99%

Differential Expression of Proteins Related with Penile Apoptosis in a Rat after Cavernous Nerve Resection

et al. 2011

View full text Add to dashboard Cite

= Abstract = Purpose: The mechanism including changes of proteome within cavernosal tissue after cavernous nerve injury were not evaluated. We performed proteomics and functional analysis to identify proteins of penile corpus cavernosum whose expression was or was not altered by cavernous nerve resection (CNR). Materials and Methods:Using 8-week-old male WKY rats, sham and CNR operation under microscope were performed. After 8 weeks, penile tissues of sham and CNR group were harvested. We used 2-DE and MALDI-TOF/TOF (AB 4700) to identify of differently expressed penile proteins. 2-DE gels were stained with silver nitrate and the gels were analyzed with PDQuest. Results:We isolated more than 950 proteins on silver-stained gels of whole protein extracts from normal rat penile corpus cavernous. Of these proteins, 48 prominent proteins were identified using MALDI-TOF/TOF. Protein characterization revealed that the most prominent penile corpus cavernous proteins were those with antioxidant, chaperone, or cytoskeletal structure. Moreover, 11 proteins having levels elevated by CNR were annexin proteins, endoplasmic reticulum protein 29, glutathione s-transferase w-1, and others. In addition, Rho-GDP dissociation inhibitor (RhoGDI), a regulator of Rho proteins, was also increased in CNR rats compared with sham-operated control rats. Conclusions:The apoptotic signals observed in penile tissues was greatly increased in CNR rats than in sham-operated rats. These results suggest that RhoGDI is one of the proteins regulated by CNR in penile smooth muscle strips, and has a crucial role in the early stage of penile apoptosis.

show abstract

“…Vcsa1 was up-regulated with each treatment (tables 1 and 2). This is of particular interest since Vcsa1 has been suggested as a marker of ED because it is down-regulated in several animal models of ED 3,12. Neither microarray analysis nor quantitative RT-PCR revealed significant changes in Slo gene expression (table 3).…”

Section: Resultsmentioning

confidence: 99%

“…Vcsa1 , one of the genes changed as a physiological response to the recovery of erectile function, has previously been suggested to be a marker of ED in several animal models 3,12. We determined whether Vcsa1 expression correlated with the recovery of erectile function after the administration of the PDE5 inhibitor tadalafil alone or in association with pVAX-hSlo.…”

Section: Resultsmentioning

confidence: 99%

Vcsa1Acts as a Marker of Erectile Function Recovery After Gene Therapeutic and Pharmacological Interventions

et al. 2009

View full text Add to dashboard Cite

Purpose We identified molecular markers of erectile function, particularly those responding to erectile dysfunction treatment. Materials and Methods Sprague-Dawley retired breeder rats were intracorporeally injected with pVAX-hSlo, pSMAA-hSlo or the control plasmid pVAX. One week later the intracorporeal pressure-to-blood pressure ratio and gene expression were determined by microarray analysis and quantitative reverse transcriptase-polymerase chain reaction. Rat corporeal cells were transfected in vitro with pVAX-hSlo, pSMAA-hSlo or pVAX and the change in gene expression was determined. We also determined whether Vcsa1 expression was changed after pharmacotherapy using tadalafil. Results Animals treated with vectors expressing hSlo had significantly improved erectile function compared to that in controls, accompanied by changed expression of a subset of genes. Vcsa1 was one of the genes that was most changed in expression (the third of approximately 31,000 with greater than 10-fold up-regulation). Changes in gene expression were different than those observed in corporeal cells transfected in vitro, distinguishing gene expression changes that were a direct effect of hSlo over expression. When tadalafil was administered in retired breeder rats, the Vcsa1 transcript increased 4-fold in corporeal tissue compared to that in untreated controls. Conclusions Our study identifies a set of genes that are changed in response to improved erectile function, rather than as a direct effect of treatment. We noted Vcsa1 may act as marker of the restoration of erectile function after gene transfer and pharmacotherapy.

show abstract

Microarray Analysis and Description of Smr1 Gene in Rat Penis in a Post-Radical Prostatectomy Model of Erectile Dysfunction

Cited by 27 publications

References 20 publications

Reversal of diabetic vasculopathy in a rat model of type 1 diabetes by opiorphin-related peptides

Reversal of diabetic vasculopathy in a rat model of type 1 diabetes by opiorphin-related peptides

Differential Expression of Proteins Related with Penile Apoptosis in a Rat after Cavernous Nerve Resection

Vcsa1Acts as a Marker of Erectile Function Recovery After Gene Therapeutic and Pharmacological Interventions

Contact Info

Product

Resources

About