Microarray Analysis of the Abscission-Related Transcriptome in the Tomato Flower Abscission Zone in Response to Auxin Depletion

Meir, Shimon; Philosoph‐Hadas, Sonia; Sundaresan, Srivignesh; Selvaraj, K; Burd, Shaul; Ophir, Ron; Kochanek, Bettina; Reid, Michael S.; Jiang, Cai‐Zhong; Lers, Amnon

doi:10.1104/pp.110.160697

Cited by 214 publications

(407 citation statements)

References 117 publications

(157 reference statements)

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“…Its ultrastructure is characterized by electronlucent ameboidal-shaped nuclei, a well developed Golgi apparatus and endoplasmic reticulum connected with an elaborate plasmodesmal system, pronounced membrane invaginations, and multivesicular and paramural bodies. In addition, polygalacturonases -enzymes involved in the execution of cell separation 4,18 are also located at the proximal side of the AZ. At the distal side of the AZ programmed cell death occurs.…”

Section: Endoreduplication In the Az Is Predominantly On Its Proximalmentioning

confidence: 99%

“…In tomato (Solanum lycopersicum) the plant hormones ethylene and auxin play central roles in regulating organ abscission by inducing and inhibiting it, respectively. Deblading of leaves or removal of flowers accelerates the abscission process by reducing the auxin supply, [3][4][5] but the process may be even faster when the plants are additionally exposed to ethylene. We have recently shown that AZs of tomato leaf or flower are structured in an asymmetric manner after abscission is triggered (Fig.…”

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Endoreduplication preferentially occurs at the proximal side of the abscission zone during abscission of tomato leaf

Dermastia

Kladnik

Bar-Dror

et al. 2012

Plant Signaling & Behavior

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Section: Endoreduplication In the Az Is Predominantly On Its Proximalmentioning

confidence: 99%

mentioning

confidence: 99%

Endoreduplication preferentially occurs at the proximal side of the abscission zone during abscission of tomato leaf

Dermastia

Kladnik

Bar-Dror

et al. 2012

Plant Signaling & Behavior

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“…When physiological changes such as senescence or maturation occur in organs, abscission is induced through reduction of auxin level and induction of ethylene signaling (Meir et al 2010;Taylor and Whitelaw 2001). Abscission is carried out by activation of cell wall degrading enzymes and remodeling proteins, including β-1,4-glucanase (cellulase; Cel), polygalacturonase (PG), xyloglucan endotransglucosylase/hydrolase (XTH), and expansin (EXP), resulting in breakdown of cell adhesion Lashbrook and Cai 2008;Meir et al 2010;Roberts et al 2002;Tucker et al 2007). These events are well conserved in different types of organs and in divergent plant species.…”

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Molecular mechanisms controlling plant organ abscission

Nakano

Ito

2013

Plant Biotechnology

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Plants shed organs by abscission, removing leaves, flowers or fruits when the organs are senescent, damaged, diseased or mature. Abscission also affects agriculture; for example, abscission of fruits or cereal grains can significantly reduce crop yield. Abscission of organs typically occurs in a predetermined tissue region, the abscission zone (AZ). Organ abscission can be disturbed in two ways, inhibition of AZ differentiation in the organ or suppression of abscission processes in AZ cells. Recent studies, mainly in Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa), and tomato (Solanum lycopersicum), have identified many genes involved in regulation of AZ differentiation and activation of abscission of flowers or floral organs, seeds, and fruits. In this review, we discuss the functions of these genes, the developmental regulation of AZ tissues, and the signaling pathways that induce abscission. We also discuss the emerging concept that the regulation of abscission involves many of the same regulators that function in determination of shoot apical meristem cell fate.

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“…Additionally, it was suggested that an important step in the abscission process is the gain of ethylene sensitivity in the AZ (Meir et al . 2010). After 24 h of ethylene treatment, the fracture in the AZ had already formed in the oldest leaves, and the process gradually spread to the younger leaves (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Both of these processes are induced by ethylene (Trobacher 2009; Meir et al . 2010; Chersicola et al . 2017).…”

Section: Introductionmentioning

confidence: 99%

The pattern of 1‐aminocyclopropane‐1‐carboxylate oxidase induction in the tomato leaf petiole abscission zone is independent of expression of the ribonuclease‐LX‐encoding LeLX gene

et al. 2018

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The abscission of tomato leaves occurs in the petiole abscission zone, and its late stage includes two spatially divided processes: cell separation and programmed cell death (PCD). Both of these processes are regulated by ethylene. The last step in ethylene biosynthesis is conversion of 1‐aminocyclopropane‐1‐carboxylic acid to ethylene, which is catalysed by the enzyme 1‐aminocyclopropane‐1‐carboxylate oxidase (ACO); however, the location of ACO in the leaf petiole abscission zone is not known. The tomato gene LeLX encodes ribonuclease LX, which is a marker for PCD and is induced by ethylene during abscission, but its association with ACO has not been explored.In a tomato transgenic line 1‐7 with inhibited expression of LeLX showing delayed leaf abscission, the morphology and ultrastructure of the leaf petiole abscission zone was examined. In this zone of the cv.’VF36’ and of a transgenic line 1‐7, spatiotemporal differences in expression of LeACO1 and LeACO4 were analysed and ACO protein was detected immunohistochemically.In comparison to wild‐type plants, there were no obvious morphological and ultrastructural features in the abscission zone of plants of a transgenic line 1‐7 before and after abscission induction. LeACO1 expression was low before abscission induction, and increased 24 h after induction, although with no apparent spatial pattern. In contrast, LeACO4 was expressed before abscission induction, and its transcript level declined 24 h after induction on the distal side of the abscission zone fracture. In the LeLX‐inhibited transgenic line, there were no significant differences in LeACO1 and LeACO4 expression in the petiole abscission zone, in comparison to wild‐type plants. In addition, the ACO protein was immunolocalised to the vascular tissues that traverse the petiole abscission zone in plants of wild type and of a transgenic line 1‐7; and additionally in the plane of future abscission zone fracture of transgenic‐line plants.The results suggest temporal differential expression of the LeACO genes in tomato leaf petioles and vascular localisation of ACO1 protein. Additionally, the results indicate that expression of LeACO genes is not affected by suppression of the LeLX expression.

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Microarray Analysis of the Abscission-Related Transcriptome in the Tomato Flower Abscission Zone in Response to Auxin Depletion

Cited by 214 publications

References 117 publications

Endoreduplication preferentially occurs at the proximal side of the abscission zone during abscission of tomato leaf

Endoreduplication preferentially occurs at the proximal side of the abscission zone during abscission of tomato leaf

Molecular mechanisms controlling plant organ abscission

The pattern of 1‐aminocyclopropane‐1‐carboxylate oxidase induction in the tomato leaf petiole abscission zone is independent of expression of the ribonuclease‐LX‐encoding LeLX gene

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