Microarray expression profiling of Arabidopsis thaliana L. in response to allelochemicals identified in buckwheat

Abstract

Buckwheat (Fagopyrum esculentum Moench) is an important annual plant cultivated for grain or as a cover crop in many countries, and it is also used for weed suppression in agro-economic systems through its release of allelochemicals. Little is known, however, concerning the mode of action of allelochemicals or plant defence response against them. Here, microarrays revealed 94, 85, and 28 genes with significantly higher expression after 6 h of exposure to the allelochemicals fagomine, gallic acid, and rutin, respectively, compared with controls. These induced genes fell into different functional categories, mainly: interaction with the environment; subcellular localization; protein with binding function or cofactor requirement; cell rescue; defence and virulence; and metabolism. Consistent with these results, plant response to allelochemicals was similar to that for pathogens (biotic stress) or herbicides (abiotic stress), which increase the concentration of reactive oxygen species (ROS; with consequent oxidative stress) in plant cells. The data indicate that allelochemicals might have relevant functions, at least in part, in the cross-talk between biotic and abiotic stress signalling because they generate ROS, which has been proposed as a key shared process between these two stress mechanisms.

Genome-wide transcriptional profiling reveals molecular signatures of secondary xylem differentiation in Populus tomentosa

“…Thus, different auxin signaling genes might have diverse functions in the regulation of secondary xylem differentiation. The diverse functions might be important for maintaining auxin balance and responding to auxin stimulus (Golisz et al, 2008;Péret et al, 2012). Genes that were more transcribed in the lignified xylem might have a function in calcium ion transport and response to auxin stimulus (Hagen and Guilfoyle, 2002), whereas auxin-related genes that were preferentially expressed in the newly formed xylem were likely to have a role in cell wall modification in response to auxin (Tamaoki et al, 2008).…”

Allelopathy, an alternative tool to improve cropping systems. A review

“…A number of recent studies have reported cellular and molecular processes involved in the production of allelochemicals and the response of target plants (Baerson et al, 2005;Dayan, 2006;Ding et al, 2007;Golisz et al, 2008;Song et al, 2008). During the molecular oxygen reduction process, intermediate molecules known as Reactive Oxygen Species (ROS) [e.g., superoxide (O .− 2 ), hydrogen peroxide (H 2 O 2 ) and hydroxyl radical (OH . )]…”

“…More recently, an analysis of the gene expression of A. thaliana though a micro-array following exposure to fagomine, gallic acid and rutin has revealed that genes expressed in response to allelochemicals share the same pathways triggered during the response to abiotic and biotic stress (Golisz et al, 2008).…”

Sorgoleone: benzoquinona lipídica de sorgo com efeitos alelopáticos na agricultura como herbicida

“…Nestes casos, a agricultura se utiliza da alelopatia do sorgo diminuindo assim o uso de controles herbicidas sintéticos danosos ao meio ambiente favorecendo dessa maneira uma produção de grãos mais saudável ao consumo. ALELOPATIA A alelopatia é a capacidade de um organismo produzir metabólitos que atuam inibindo ou estimulando crescimento ou o desenvolvimento de outros organismos, que estão próximos e que competem por recursos limitados como: nutrientes, água e luz golisz et al, 2008).…”