Microbes as a boon for the bane of heavy metals

Dave, Divya; Sarma, Sameera; Parmar, Paritosh; Shukla, Arpit; Goswami, Dweipayan; Shukla, A. D.; Saraf, Meenu

doi:10.1007/s42398-020-00112-2

Cited by 19 publications

(11 citation statements)

References 119 publications

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“…5 b, Th-tolerance by AM3 was substantially improved in optM Th+ with a slight increase in its biomass. The isolate was able to resist and thrive at 1500 mg L −1 of Th, which is distinct from the results obtained in NB Th+ medium which may be attributed to an increase in nitrogen content of the medium 8 . Also, the extraneous factors, which may have a negative effect on the growth, were corrected during the optimization process making supplementation of Th; the only stress to the bacterium in optM Th+ .…”

Section: Resultscontrasting

confidence: 91%

“…Microorganisms, being survivor of various calamities on earth over the time, are being exploited in remediation of radwaste owing to their advantages of being environment friendly and easy to grow, in addition to being amenable and adaptable while retaining their self-sustenance with generation of no or minimal polluting by-products 7 . Bacterial resistance to radionuclides has intrigued researchers globally 2 , 8 . In recent years, studies pertaining to the devastating Chernobyl accident has led to the discovery of a distinctive phenomenon of ‘ radiostimulation ’, where Cryptococcus neoformans , a fungus, showed preference of a radiotrophic mode of nutrition over the conventional chemotrophism 9 – 11 .…”

Section: Introductionmentioning

confidence: 99%

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Exemplifying an archetypal thorium-EPS complexation by novel thoriotolerant Providencia thoriotolerans AM3

Shukla

Parmar

Goswami

et al. 2021

Sci Rep

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It is the acquisition of unique traits that adds to the enigma of microbial capabilities to carry out extraordinary processes. One such ecosystem is the soil exposed to radionuclides, in the vicinity of atomic power stations. With the aim to study thorium (Th) tolerance in the indigenous bacteria of such soil, the bacteria were isolated and screened for maximum thorium tolerance. Out of all, only one strain AM3, found to tolerate extraordinary levels of Th (1500 mg L−1), was identified to be belonging to genus Providencia and showed maximum genetic similarity with the type strain P. vermicola OP1T. This is the first report suggesting any bacteria to tolerate such high Th and we propose to term such microbes as ‘thoriotolerant’. The medium composition for cultivating AM3 was optimized using response surface methodology (RSM) which also led to an improvement in its Th-tolerance capabilities by 23%. AM3 was found to be a good producer of EPS and hence one component study was also employed for its optimization. Moreover, the EPS produced by the strain showed interaction with Th, which was deduced by Fourier Transform Infrared (FTIR) spectroscopy.

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Section: Resultscontrasting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Exemplifying an archetypal thorium-EPS complexation by novel thoriotolerant Providencia thoriotolerans AM3

Shukla

Parmar

Goswami

et al. 2021

Sci Rep

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“…Heavy metals, such as organic pollutants, are not degradable but can be transformed to exist in less toxic form. Microbes are the cheap weapon since they change quickly to overcome heavy metal pressure by creating appropriate survival techniques, like sequestration or active metal transport [ 34 ]. The key sources of pollutants in water quality are heavy metal ions like Pb 2+ and Cd 2+ .…”

Section: Discussionmentioning

confidence: 99%

Construction of a sensitive and specific lead biosensor using a genetically engineered bacterial system with a luciferase gene reporter controlled by pbr and cadA promoters

et al. 2020

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Background A bacterial biosensor refers to genetically engineered bacteria that produce an assessable signal in the presence of a physical or chemical agent in the environment. Methods We have designed and evaluated a bacterial biosensor expressing a luciferase reporter gene controlled by pbr and cadA promoters in Cupriavidus metallidurans (previously termed Ralstonia metallidurans) containing the CH34 and pI258 plasmids of Staphylococcus aureus, respectively, and that can be used for the detection of heavy metals. In the present study, we have produced and evaluated biosensor plasmids designated pGL3-luc/pbr biosensor and pGL3-luc/cad biosensor, that were based on the expression of luc+ and under the control of the cad promoter and the cadC gene of S. aureus plasmid pI258 and pbr promoter and pbrR gene from plasmid pMOL30 of Cupriavidus metallidurans. Results We found that the pGL3-luc/pbr biosensor may be used to measure lead concentrations between 1–100 μM in the presence of other metals, including zinc, cadmium, tin and nickel. The latter metals did not result in any significant signal. The pGL3-luc/cad biosensor could detect lead concentrations between 10 nM to 10 μM. Conclusions This biosensor was found to be specific for measuring lead ions in both environmental and biological samples.

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“…Heavy metals, such as organic pollutants, are not degradable but can be transformed to exists in less toxic form. Microbes are the cheap weapon since they change quickly to overcome heavy metal pressure by creating appropriate survival techniques, like sequestration or active metal transport [23]. The key sources of pollutants in water quality are heavy metal ions like Pb 2+ and Cd 2+.…”

Section: Discussionmentioning

confidence: 99%

Construction of a sensitive and specific lead biosensor using a genetically engineered bacterial system with a luciferase gene reporter controlled by pbr and cadA promoters

Nourmohammadi

Hosseinkhani

Khoshdel‐Sarkarizi

et al. 2020

Preprint

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Background: A bacterial biosensor refers to genetically engineered bacteria that produce an assessable signal in the presence of a physical or chemical agent in the environment.Methods: We have designed and evaluated a bacterial biosensor expressing a luciferase-reporter gene controlled by pbr and cadA promoters in Cupriavidus metallidurans (previously termed Ralstonia metallidurans ) containing the CH34 and pI258 plasmids of Staphylococcus aureus , respectively, and that can be used for the detection of heavy metals. In the present study, we have produced and evaluated biosensor plasmids designated pGL3-luc/pbr -biosensor and pGL3-luc/cad -biosensor, that were based on the expression of luc+ under the control of the cad promoter and the cadC gene of S. aureus plasmid pI258 and pbr promoter and pbrR gene from plasmid pMOL30 of Cupriavidus metallidurans.Results: We found that the pGL3-luc/pbr -biosensor may be used to measure lead concentrations between 1-100 μM in the presence of other metals, including: zinc, cadmium, tin and nickel. The latter metals did not result in any significant signal gene expression of the reporter . The pGL3-luc/cad -biosensor could detect lead concentrations between 10 nM to 10 μM.Conclusions: This biosensor was found to be a specific for measuring lead ions in both environmental and biological samples.

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Microbes as a boon for the bane of heavy metals

Cited by 19 publications

References 119 publications

Exemplifying an archetypal thorium-EPS complexation by novel thoriotolerant Providencia thoriotolerans AM3

Exemplifying an archetypal thorium-EPS complexation by novel thoriotolerant Providencia thoriotolerans AM3

Construction of a sensitive and specific lead biosensor using a genetically engineered bacterial system with a luciferase gene reporter controlled by pbr and cadA promoters

Construction of a sensitive and specific lead biosensor using a genetically engineered bacterial system with a luciferase gene reporter controlled by pbr and cadA promoters

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