Microbial biofilms on facial prostheses

Abstract

The composition of microbial biofilms on silicone rubber facial prostheses was investigated and compared with the microbial flora on healthy and prosthesis-covered skin. Scanning electron microscopy showed the presence of mixed bacterial and yeast biofilms on and deterioration of the surface of the prostheses. Microbial culturing confirmed the presence of yeasts and bacteria. Microbial colonization was significantly increased on prosthesis-covered skin compared to healthy skin. Candida spp. were exclusively isolated from prosthesis-covered skin and from prostheses. Biofilms from prostheses showed the least diverse band-profile in denaturing gradient gel electrophoresis (DGGE) whereas prosthesis-covered skin showed the most diverse band-profile. Bacterial diversity exceeded yeast diversity in all samples. It is concluded that occlusion of the skin by prostheses creates a favorable niche for opportunistic pathogens such as Candida spp. and Staphylococcus aureus. Biofilms on healthy skin, skin underneath the prosthesis and on the prosthesis had a comparable composition, but the numbers present differed according to the microorganism.

In vitrophenotypic differentiation towards commensal and pathogenic oral biofilms

“…After elution, the DNA concentration was estimated by determining the absorbance at 260 nm using the Nanodrop (Isogen, De Meern, the Netherlands, ND-1000). The primers used for DGGE PCR amplification were as used previously (Ariani et al 2012) and are listed in Table 1. PCR was performed in a final volume of 25 μl containing 1 μl of each primer (10 mM), 1 μl of dNTP (10 mM), 2.5 μl of 10× DreamTaq buffer including MgCl 2 (Thermo Scientific, Vilnius, Lithuania), 0.2 μl of DreamTaq DNA polymerase (5 U μl −1 , Thermo Scientific) and 50 ng of DNA.…”

Combination prosthetic design providing a superior retention for mid-facial defect rehabilitation: A Case Report

“…Furthermore, the palatal bulb of the obturator designed to hang at the scar band of the extraoral defect reduced the cantilever effect, which helped in preservation of the periodontal health of the remaining abutment teeth. The design using two magnets and one locator attachments provided adequate retention for the facial prosthesis without using medical adhesive and reduced the silicone-skin contact area, which prevents the risk of physical irritant contact dermatitis (14). In addition, the lightweight combination prostheses was gained by fabricating a bulbless obturator …”

“…In addition, this condition causes permanent black discoloration and silicone deterioration (14,15). In our design, we aim to reduce the fungal colonization by extending the acrylic framework underneath the facial silicone to cover the part of nasal cavity, and thereby prevent the direct exposure of the silicone to the moisture from respiration and body temperature.…”

Liposome Based Drug Delivery Systems for Control of Microbial Biofilms

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