Microbial cell factories for bio-based biodegradable plastics production

Han, Xiao; Liu, Jiongqin; Tian, Shen; Tao, Fei; Xu, Ping

doi:10.1016/j.isci.2022.105462

Cited by 11 publications

(4 citation statements)

References 131 publications

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“…In recent years, metabolic engineering approaches have been conducted to improve adipic acid production (Zhao et al 2018 ); also, an artificial biosynthesis system was established to catalyze the synthesis reaction of cyclohexane to adipic acid in Escherichia coli (Wang et al 2020 ). With the increasing attention to environmental protection, degradable bio-based materials are widely attracting people’s attentions (Han et al 2022 ), just as the global production capacity of poly-lactic acid (PLA) is expanding. Due to their biocompatibility and biodegradability, polyhydroxyalkanoates (PHA) have the widespread applications in the areas of chemical industry, medical, etc.…”

Section: Discussionmentioning

confidence: 99%

Modular metabolic engineering of Bacillus amyloliquefaciens for high-level production of green biosurfactant iturin A

She,

Zhou,

Dong

et al. 2024

Appl Microbiol Biotechnol

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As a kind of biosurfactants, iturin A has attracted people’s wide attentions due to their features of biodegradability, environmentally friendly, etc.; however, high production cost limited its extensive application, and the aim of this research wants to improve iturin A production in Bacillus amyloliquefaciens. Firstly, dual promoter was applied to strengthen iturin A synthetase expression, and its yield was increased to 1.25 g/L. Subsequently, original 5′-UTRs of downstream genes (ituA, ituB, and ituC) in iturin A synthetase cluster were optimized, which significantly increased mRNA secondary stability, and iturin A yield produced by resultant strain HZ-T3 reached 2.32 g/L. Secondly, synthetic pathway of α-glucosidase inhibitor 1-deoxynojirimycin was blocked to improve substrate corn starch utilization, and iturin A yield was increased by 34.91% to 3.13 g/L. Thirdly, efficient precursor (fatty acids, Ser, and Pro) supplies were proven as the critical role in iturin A synthesis, and 5.52 g/L iturin A was attained by resultant strain, through overexpressing yngH, serC, and introducing ocD. Meanwhile, genes responsible for poly-γ-glutamic acid, extracellular polysaccharide, and surfactin syntheses were deleted, which led to a 30.98% increase of iturin A yield. Finally, lipopeptide transporters were screened, and iturin A yield was increased by 17.98% in SwrC overexpression strain, reached 8.53 g/L, which is the highest yield of iturin A ever reported. This study laid a foundation for industrial production and application development of iturin A, and provided the guidance of metabolic engineering breeding for efficient production of other metabolites synthesized by non-ribosomal peptide synthetase. Key points • Optimizing 5′-UTR is an effective tactics to regulate synthetase cluster expression. • Blocking 1-DNJ synthesis benefited corn starch utilization and iturin A production. • The iturin A yield attained in this work was the highest yield reported so far.

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Section: Discussionmentioning

confidence: 99%

Modular metabolic engineering of Bacillus amyloliquefaciens for high-level production of green biosurfactant iturin A

She,

Zhou,

Dong

et al. 2024

Appl Microbiol Biotechnol

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“…Researchers have attempted several methods to solve this issue but few of them achieved desired results [ 1 ]. Therefore, there is an urgent need for safe and environmental-friendly novel materials to replace traditional plastics to solve a series of environmental issues [ 2 ]. In this situation, polyhydroxyalkanoates (PHAs), the only polyesters that are completely synthesized biologically, stand out due to their excellent features equivalent to petroleum-based plastics [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Co-utilization of glucose and xylose for the production of poly-β-hydroxybutyrate (PHB) by Sphingomonas sanxanigenens NX02

Ming,

Li,

Shi

et al. 2023

Microb Cell Fact

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Background Poly-β-hydroxybutyrate (PHB), produced by a variety of microbial organisms, is a good substitute for petrochemically derived plastics due to its excellent properties such as biocompatibility and biodegradability. The high cost of PHB production is a huge barrier for application and popularization of such bioplastics. Thus, the reduction of the cost is of great interest. Using low-cost substrates for PHB production is an efficient and feasible means to reduce manufacturing costs, and the construction of microbial cell factories is also a potential way to reduce the cost. Results In this study, an engineered Sphingomonas sanxanigenens strain to produce PHB by blocking the biosynthetic pathway of exopolysaccharide was constructed, and the resulting strain was named NXdE. NXdE could produce 9.24 ± 0.11 g/L PHB with a content of 84.0% cell dry weight (CDW) using glucose as a sole carbon source, which was significantly increased by 76.3% compared with the original strain NX02. Subsequently, the PHB yield of NXdE under the co-substrate with different proportions of glucose and xylose was also investigated, and results showed that the addition of xylose would reduce the PHB production. Hence, the Dahms pathway, which directly converted D-xylose into pyruvate in four sequential enzymatic steps, was enhanced by overexpressing the genes xylB, xylC, and kdpgA encoding xylose dehydrogenase, gluconolactonase, and aldolase in different combinations. The final strain NX02 (ΔssB, pBTxylBxylCkdpgA) (named NXdE II) could successfully co-utilize glucose and xylose from corn straw total hydrolysate (CSTH) to produce 21.49 ± 0.67 g/L PHB with a content of 91.2% CDW, representing a 4.10-fold increase compared to the original strain NX02. Conclusion The engineered strain NXdE II could co-utilize glucose and xylose from corn straw hydrolysate, and had a significant increase not only in cell growth but also in PHB yield and content. This work provided a new host strain and strategy for utilization of lignocellulosic biomass such as corn straw to produce intracellular products like PHB.

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“…Currently, the utilization of LA as the monomer for the production of poly-lactide (PLA) polymers has increased remarkably due to the rising demand for biodegradable plastics (de Albuquerque et al, 2021;Huang et al, 2023b). The physical properties of PLA depend on its D-LA and L-LA composition (Farah et al, 2016;Han et al, 2022). Poly L-lactic acid (PLLA) is a thermostable L-LA-based homopolymer widely used for food packaging, e.g., food containers, blister packaging, and disposable cups (He et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Microbial production of stereospecific lactic acid from sugarcane trash hydrolysate with no pre-detoxification step

Boonyapakron,

Prabmark,

Bunterngsook

et al. 2023

Appl Env Biotechnol

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Sugarcane trash (SCT) is a promising underused lignocellulosic material for the production of chemicals in biorefineries. In this work, we studied the use of SCT to produce lactic acid (LA), an important commodity chemical used in food and bioplastic industries. SCT was pretreated with a hydrothermal liquid hot water step followed by saccharification with Cellic® CTec2, which resulted in the SCT hydrolysate containing 30.42±0.08 g/L total sugar, equivalent to 88.10% and 42.92% glucose and xylose recoveries, respectively. Screening of stereospecific LA production exhibited Lactococcus lactis BCC 68868 and Leuconostoc lactis BCC 62792 efficiently utilized SCT hydrolysate converted to entirely L-LA and D-LA, respectively. A detoxification step was unnecessary for hydrolysate fermentation to LA, and the addition of CaCO3 for pH control enabled complete hydrolysate utilization. La. lactis BCC 68868 converted SCT hydrolysate into optically pure L-LA at 19.34 g/L, equivalent to a conversion yield of 0.95 g LA/g sugar. While Le. lactis BCC 62792 produced D-LA at 10.39 g/L, equivalent to a conversion yield of 0.45 g LA/g sugar with ethanol and acetic acid as the by-products, suggesting their homofermentative and heterofermentative characteristic, respectively. This work demonstrates a promising strategy to produce enantiomerically defined LA from this underutilized agricultural waste.

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Microbial cell factories for bio-based biodegradable plastics production

Cited by 11 publications

References 131 publications

Modular metabolic engineering of Bacillus amyloliquefaciens for high-level production of green biosurfactant iturin A

Modular metabolic engineering of Bacillus amyloliquefaciens for high-level production of green biosurfactant iturin A

Co-utilization of glucose and xylose for the production of poly-β-hydroxybutyrate (PHB) by Sphingomonas sanxanigenens NX02

Microbial production of stereospecific lactic acid from sugarcane trash hydrolysate with no pre-detoxification step

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