Microbial cellulases: Engineering, production and applications

Juturu, Veeresh; Wu, Jin

doi:10.1016/j.rser.2014.01.077

Cited by 383 publications

(202 citation statements)

References 87 publications

Supporting

Mentioning

183

Contrasting

Unclassified

Order By: Relevance

“…3.2.1.176) and (E.C. 3.2.1.21), respectively (Garvey et al 2013;Juturu and Wu 2014). The cleavage of internal bonds of the cellulose chain is catalyzed by endoglucanases (endo-1,4-β-glucanases) that catalyze randomly, while the ends of the chain are cleaved by the exoglucanases (exo-1,4-β-glucanases), which release cellobiose.…”

Section: Thermostable Cellulase Familymentioning

confidence: 99%

“…Apart from this, many researchers have also reported other applications of cellulosomal enzymes including removal of bacterial biofilms (Juturu and Wu 2014); enhancement of mechanical and physical properties of paper (Cadena et al 2010;Shi et al 2014); in textile, detergent and food processing (Juturu and Wu 2014;Karmakar and Ray 2011;Kuhad et al 2011;Oliveira et al 2015).…”

Section: Applications Of Cellulosomesmentioning

confidence: 99%

See 1 more Smart Citation

Bioprospecting thermostable cellulosomes for efficient biofuel production from lignocellulosic biomass

Arora

Behera

Sharma

et al. 2015

Bioresour. Bioprocess.

View full text Add to dashboard Cite

The adverse climatic conditions due to continuous use of fossil-derived fuels are the driving factors for the development of renewable sources of energy. Current biofuel research focuses mainly on lignocellulosic biomass (LCB) such as agricultural, industrial and municipal solid wastes due to their abundance and renewability. Although many mesophilic cellulolytic microorganisms have been reported, efficient and economical bioconversion to simple sugars is still a challenge. Thermostable cellulolytic enzymes play an indispensible role in degradation of the complex polymeric structure of LCB into fermentable sugar stream due to their higher flexibility with respect to process configurations and better specific activity than the mesophilic enzymes. In some anaerobic thermophilic/thermotolerant microorganisms, few cellulases are organized as unique multifunctional enzyme complex, called the cellulosome. The use of cellulosomal multienzyme complexes for saccharification seems to be a promising and cost-effective alternative for complete breakdown of cellulosic biomass. This paper aims to explore and review the important findings in cellulosomics and forward the path for new cutting-edge opportunities in the success of biorefineries. Herein, we summarize the protein structure, regulatory mechanisms and their expression in the host cells. Furthermore, we discuss the recent advances in specific strategies used to design new multifunctional cellulosomal enzymes, which can function as lignocellulosic biocatalysts and evaluate the roadblocks in the yield and stability of such designer thermozymes with overall progress in lignocellulose-based biorefinery.

show abstract

Section: Thermostable Cellulase Familymentioning

confidence: 99%

Section: Applications Of Cellulosomesmentioning

confidence: 99%

Bioprospecting thermostable cellulosomes for efficient biofuel production from lignocellulosic biomass

Arora

Behera

Sharma

et al. 2015

Bioresour. Bioprocess.

View full text Add to dashboard Cite

show abstract

“…Endoglucanases are of special interest because of their ability to initiate cellulose hydrolysis, and their action on the amorphous regions of crystalline cellulose matrix is considered the rate-limiting step of cellulose utilization (Malherbe and Cloete 2002). Furthermore, endoglucanases have special applications in textile and food processing industries (Juturu and Wu 2014;Puranen et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Endoglucanase Production by Bacillus aerius on Mixed Lignocellulosic Substrates

2016

View full text Add to dashboard Cite

Selected carbon sources including soluble carboxymethyl cellulose (CMC), insoluble microcrystalline cellulose (MCC), and single (SS)/mixed lignocellulosic substrates (MS), were evaluated for endoglucanase production by B. aerius S5.2. The lignocellulosic substrates of oil palm empty fruit bunch (EFB), oil palm frond (OPF), rice husk (RH), and their mixture (MS) supported growth of the strain better than CMC and MCC. The maximum endoglucanase activity on MS was 7.3-, 2.6-, 1.7-, and 1.2-fold higher than those recorded on MCC, CMC, EFB/OPF, and RH, respectively. While the reducing sugar concentration of the CMC medium was comparable to that of the EFB and MS media, wide variability was observed in the reducing sugar concentrations among the lignocellulosic substrates. Extremely low levels of sugar were detected in the MCC medium, reflecting its poor digestibility and hence unsuitability for growth and endoglucanase production. Endoglucanase production was predominantly extracellular when the strain was grown on CMC and MS. After seven days of fermentation, there was an approximately 25% reduction in MS dry weight. These findings show that the use of mixed lignocellulosics could potentially reduce the cost of cellulase production. Certain novel aspects of the cellulase system of B. aerius are reported in this study.

show abstract

“…Lignocelluloses, the most promising renewable resources, are obtained from various sources (Juturu and Wu 2014). The agricultural sources such as corn stalks and grasses have received much attention.…”

Section: Introductionmentioning

confidence: 99%

Pyrrolidinium Ionic Liquids as Effective Solvents for Lignin Extraction and Enzymatic Hydrolysis of Lignocelluloses

et al. 2016

BioResources

View full text Add to dashboard Cite

show abstract

Microbial cellulases: Engineering, production and applications

Cited by 383 publications

References 87 publications

Bioprospecting thermostable cellulosomes for efficient biofuel production from lignocellulosic biomass

Bioprospecting thermostable cellulosomes for efficient biofuel production from lignocellulosic biomass

Enhanced Endoglucanase Production by Bacillus aerius on Mixed Lignocellulosic Substrates

Pyrrolidinium Ionic Liquids as Effective Solvents for Lignin Extraction and Enzymatic Hydrolysis of Lignocelluloses

Contact Info

Product

Resources

About