Microbial communities affecting albumen photography heritage: a methodological survey

Puškárová, Andrea; Bučková, Maria; Habalová, Božena; Kraková, Lucia; Maková, Alena; Pangallo, Domenico

doi:10.1038/srep20810

Cited by 53 publications

(42 citation statements)

References 54 publications

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“…Bacteria were identified using the 16S rRNA gene, which was amplified using the primers 27f (5 0 -AGA GTT TGA TCC TGG CTC AG-3 0 ) and 685r (5 0 -TCT ACG CAT TTC ACC GCT AC-3 0 ). Full details of both identification strategies are given in Puškárová et al (2016).…”

Section: Dna Extraction Selection and Identification Of Isolatesmentioning

confidence: 99%

The microbiomes of a XVIII century mummy from the castle of Krásna Hôrka (Slovakia) and its surrounding environment

Kraková

Šoltýs

Puškárová

et al. 2018

Environmental Microbiology

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This microbiological survey was performed to determine the conservation state of a mummy in the Slovak castle of Krásna Hôrka and its surrounding environment. Culture-dependent identification was coupled with biodegradation assays on keratin, gelatin and cellulose. Next Generation Sequencing (NGS) using Illumina platform was used for a deeper microbial investigation. Three environmental samples were collected: from the glass of the sarcophagus, from the air inside it, and from the air of the chapel where the mummy is located. Seven different samples were taken from mummy's surface: from the left ear, left-hand palm, left-hand nail, left instep, right hand, abdomen and mineral crystals embedded within the skin. Three internal organ samples, from the lung, pleura and stomach, were also included in this study. Together, the culture-dependent and culture-independent analyses revealed that the bacterial communities present had fewer taxa than the fungal ones. The mycobiome showed the largest variability and included Epicoccum nigrum, Penicillium spp., Alternaria spp., Aspergillus spp., Cladosporium spp. and Aureobasidium pullulans; many other Ascomycota and Basidiomycota genera were detected by NGS. The most interesting results came from the skin mineral crystals and the internal organs. The hydrolytic assays revealed those microorganisms which might be considered dangerous 'mummy pathogens'. © 2018 Society for Applied Microbiology and John Wiley & Sons Ltd.

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Section: Dna Extraction Selection and Identification Of Isolatesmentioning

confidence: 99%

The microbiomes of a XVIII century mummy from the castle of Krásna Hôrka (Slovakia) and its surrounding environment

Kraková

Šoltýs

Puškárová

et al. 2018

Environmental Microbiology

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“…nylon membranes), DNA extraction and PCR amplification did not indicate contamination. Binding membranes have been so far applied for bacterial and fungal sampling from various surfaces [ 38 – 40 ]. The risk of sampling microbial DNA from the palm leaf manuscript was valid as SEM analyses showed fungal infestation ( Fig 4D ).…”

Section: Resultsmentioning

confidence: 99%

“…The usage of binding membranes offers another non-invasive sampling method. Nitrocellulose and nylon membranes have been applied for bacterial and fungal sampling from the surface of paintings [ 38 ], manuscripts [ 39 ], and photographs [ 40 ] so far, but have not been tested in their efficiency to bind endogenous DNA.…”

Section: Introductionmentioning

confidence: 99%

Not stealing from the treasure chest (or just a bit): Analyses on plant derived writing supports and non-invasive DNA sampling

Schulz¹,

Lautner

Fromm

et al. 2018

PLoS ONE

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Written communication plays a crucial role in the history of modern civilizations as manuscripts do not only exist contemporarily, but are passed on to subsequent generations. Besides a document’s content, information is stored in the materials used for its production. Analyses of the composition allow, for example, identifying the biological origins of materials, dating, and help to understand degradation patterns. A combination of microscopic and DNA approaches was applied in order to analyze various plant derived writing sheets. Given their diversity and abundance in museum collections, plant based writing supports are yet an underexplored target for DNA studies. DNA retrieval of paper is low compared to raw paper plant material, which is likely due to the loss of organic components during paper production. Optimizing DNA extraction for each respective material drastically increased DNA recovery. Finally, we present a non-invasive DNA sampling method that utilizes nylon membranes, commonly used for bacterial DNA sampling and that is applicable to delicate material. Although bacterial infestation was visible on one sample, as indicated by scanning electron microscopy, endogenous DNA was retrieved. The results presented here are promising as they extend the scope of sources for DNA analyses by demonstrating that DNA molecules can be retrieved from a variety of plant derived writing supports. In future, such analyses can help to explore the biological diversity not only of plants and of additives utilized for producing writing supports, but also of the plenty products made from paper.

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“…To depict the bacterial diversity colonizing clay tiles, we conducted isolation and characterization of bacterial strains. The isolation of bacterial strains on tiles followed routine and previously described protocols used to isolate strain on building materials (Puškárová et al, 2016;Urzì et al, 2016). Briefly, for each sample, 100 µL of the solution collected previously was inoculated on Petri dishes containing R2A culture medium (BD Difco) (Puškárová et al, 2016;Urzì et al, 2016) with 100 mg.L -1 cycloheximide (Urzì et al, 2016) and 4 mg.L -1 benomyl (Lage and Bondoso, 2012) or solid BG11 culture medium (BG11 50× (Sigma Aldrich) supplemented with 15 g.L -1 agar) (Rippka et al, 1979;Urzì et al, 2016) with 100 mg.L -1 cycloheximide (Urzì et al, 2016).…”

Section: Isolation Of Bacterial Strains By Culture-based Techniquesmentioning

confidence: 99%

High bacterial diversity in pioneer biofilms colonizing ceramic roof tiles

Romani

Carrion

Fernandez

et al. 2019

International Biodeterioration & Biodegradation

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Ceramic roof tiles are extremely common building materials that are subjected to the natural phenomenon of biodeterioration, which initially modifies the tile surface and ultimately causes its destruction. The bacterial diversity of the visible biofilm responsible for biodeterioration has been previously examined. In contrast, the early stages of tile colonization and pioneer biofilm growth on these surfaces have been poorly explored. To investigate these pioneering stages of bacterial tile colonization, we combined imagery and conventional culture-based approaches, as well as Illumina-based high-throughput sequencing methods to examine samples collected from unexposed new tiles and tiles that were subjected to few-months outdoor exposure. In all the samples, we observed a pioneering biofilm including a significant bacterial diversity, on both new materials and those subjected to slight exposure, with a total of 279 and 411 different OTUs detected, respectively. This pioneer diversity was dominated by Proteobacteria (more than 50% of the total bacterial diversity) and, at the genus level, by Sphingomonas and the genus 1174-901-12 related to the Beijerinckiaceae. Interestingly, the major patterns of the observed bacterial diversity remained similar between samples collected from unexposed and exposed tiles. Collectively, these data clearly indicate the need to focus on the pioneer colonizing bacteria that form the initial biofilm on building materials, which can subsequently lead to mature biofilm formation and visible biodeterioration.

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Microbial communities affecting albumen photography heritage: a methodological survey

Cited by 53 publications

References 54 publications

The microbiomes of a XVIII century mummy from the castle of Krásna Hôrka (Slovakia) and its surrounding environment

The microbiomes of a XVIII century mummy from the castle of Krásna Hôrka (Slovakia) and its surrounding environment

Not stealing from the treasure chest (or just a bit): Analyses on plant derived writing supports and non-invasive DNA sampling

High bacterial diversity in pioneer biofilms colonizing ceramic roof tiles

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