Microbial community analysis: a kinetic approach to constructing potential C source utilization patterns

Lindstrom, Jon E.; Barry, Ronald P.; Braddock, Joan F.

doi:10.1016/s0038-0717(97)00113-2

Cited by 96 publications

(81 citation statements)

References 9 publications

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“…Using these values the average well colour development (AWCD) was calculated. The community-level physiological profile (CLPP) data was analysed estimating kinetic parameters by fitting the OD 590 vs. time curve to a density dependent logistic growth equation [32]:…”

Section: Metabolic Diversitymentioning

confidence: 99%

“…These parameters provide information on different aspects of the carbon source utilization: the asymptote (K) is an estimate of the potential maximum amount of substrate used, the exponential rate parameter (R) provides information on how rapidly a carbon source is metabolized by a community once the density has reached the level at which colour production begins, and the time to reach the midpoint (S) supplies information about the initial inoculum density and the relative growth rates of the species able to use the carbon source in each well [13,32]. Kinetic parameters may be the most useful format for Biolog data, since they are weakly correlated with each other [13].…”

Section: Metabolic Diversitymentioning

confidence: 99%

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Influence of seasons and land-use practices on soil microbial activity and metabolic diversity in the “Montado ecosystem”

Costa¹,

Freitas²,

Sousa³

2013

European Journal of Soil Biology

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a b s t r a c tThe "Montado ecosystem" is important both economically and ecologically; this ecosystem is dominated by cork and holm oak trees (Quercus suber L. and Quercus rotundifolia Lam. respectively) combined with a rotation of crops/fallow/pastures. Diverse management strategies, deviating from the sustainable use of the ecosystem, have been implemented, from which arise some extreme situations of over-use or abandonment. To evaluate the effects of different soil use and management, namely extensive cropping, intensive pasture and abandonment, in the activity of soil microorganisms, dehydrogenase, acid phosphatase, b-glucosidase and urease activities, N-mineralization and nitrification rates were measured in different land-use practices, in different seasons (winter, spring and autumn). Also, the potential metabolic diversity was evaluated by analysis of community-level physiological profiles (CLPPs). Seasonal effects were evident with maximum activity occurring in rainy seasons (winter and autumn) and lower substrate utilization in winter. Significant correlations between most microbial parameters and soil water content reflect this seasonal effect. Although showing mainly a seasonal change, microbial parameters were able to distinguish the abandoned area, with a general low activity and differential exponential rates in the use of several substrates, such as amino acids, miscellaneous and polymers, probably associated with changes in organic matter quality.

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Section: Metabolic Diversitymentioning

confidence: 99%

Section: Metabolic Diversitymentioning

confidence: 99%

Influence of seasons and land-use practices on soil microbial activity and metabolic diversity in the “Montado ecosystem”

Costa¹,

Freitas²,

Sousa³

2013

European Journal of Soil Biology

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“…Doubts have also been expressed about the validity of straightforward collection and interpretation of Biolog plate readings (positive or negative for utilization), and kinetics analysis suggested as a means to address this issue (Lawley and Bell 1998;Lindstrom et al 1998). No steps were taken in this study to account for differences in the rate of microbial response to Biolog substrates.…”

Section: Microbial Metabolic Diversitymentioning

confidence: 99%

The effects of a crude oil spill on microbiological indices of soil biological quality

Timmerman¹,

Fuller²,

Burton³

2003

Can. J. Soil. Sci.

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. 2003. The effects of a crude oil spill on microbiological indices of soil biological quality. Can. J. Soil Sci. 83: 173-181. The effects of a crude oil spill and remediation on the biological quality of an agricultural soil were examined based on three microbiological indices: microbial biomass carbon (MBC), dehydrogenase activity (DHA) and microbial metabolic diversity (MMD). The soils in both contaminated and uncontaminated plots were clay loam Gleyed Rego Black Chernozems developed from lacustrine materials overlying till. The four remedial treatments consisted of: meadow bromegrass (Bromus biebersteinii. Rohman and Schult), alfalfa (Medicago sativa L. cv. 'Algonquin'), fallow with wheat straw incorporation (SF) and unamended fallow (UF SP ). An unamended fallow on adjacent uncontaminated land served as a control (UF CON ). The spill had a statistically significant, negative effect on MBC and MMD (P = 0.01 and 0.05, respectively). Although a negative effect on DHA was not significant (P = 0.05), the results show evident trends. The forage treatments were, overall, significantly more effective than the SF treatment in restoring soil biological quality in hydrocarbon contaminated soil to control levels. Based on overall trends only, bromegrass had the greatest effect on soil biological quality relative to the UF SP , followed by alfalfa. The incorporation of wheat straw did not significantly affect any of the examined microbial indices. . Même si les effets négatifs de la pollution sur la DHA n'é-taient pas significatifs (P = 0,05), certaines tendances se dégagent des résultats. Dans l'ensemble, la culture d'espèces fourragères restaure la qualité biologique des sols contaminés par les hydrocarbures à celle de la parcelle témoin sensiblement mieux que le traitement SF. Si l'on ne se fie qu'aux tendances générales, le brome améliore le plus la qualité biologique du sol comparativement à la UF SP , suivi de la luzerne. L'incorporation de paille de blé au sol n'améliore pas significativement les indices de la qualité microbiologique du sol examinés.

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“…The plates were incubated at 28°C in the dark and absorbance at 405 nm was read using an ELISA plate reader (Model 550, Bio-Rad, CA) at 4 to 16 h intervals for 78 h. During the incubation, the plates were wrapped in a plastic film to avoid desiccation. The average well color development (AWCD, Garland & Mills, 1991) and the kinetic (Lindstrom et al, 1998) approaches were applied. An AWCD for a sample was the average of absorbance values at 405 nm for the carbon sources at a particular time.…”

mentioning

confidence: 99%

“…Using the computer software, SPSS 10.0.5J (SPSS Japan, Inc., Tokyo, Japan), the kinetic parameters (Lindstrom et al, 1998) were determined. The criteria described by Mondini and Insam (2003) were applied for accessing model fitness.…”

mentioning

confidence: 99%

Effects of air-drying soils on discriminating the soils with community-level physiological profiling

Doi

2005

J. Gen. Appl. Microbiol.

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Microbial community analysis: a kinetic approach to constructing potential C source utilization patterns

Cited by 96 publications

References 9 publications

Influence of seasons and land-use practices on soil microbial activity and metabolic diversity in the “Montado ecosystem”

Influence of seasons and land-use practices on soil microbial activity and metabolic diversity in the “Montado ecosystem”

The effects of a crude oil spill on microbiological indices of soil biological quality

Effects of air-drying soils on discriminating the soils with community-level physiological profiling

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