Microbial community composition, dynamics, and biogeochemistry during the start-up of a partial nitritation-anammox pathway in an upflow reactor

Ribeiro, H. L. C.; Wijaya, I Made Wahyu; Soares-Santos, Verónica; Soedjono, Eddy Setiadi; Slamet, Agus; Teixeira, Catarina; Bordalo, Adriano A.

doi:10.1186/s42834-022-00130-1

Cited by 8 publications

(5 citation statements)

References 41 publications

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“…The sequencing also revealed other changes in the microbial population. Obligately anaerobic thermophiles from class Anaerolineae (Nunoura et al, 2013) and other obligately anaerobic bacterium PHOS-HE36 from class Ignavibacteria, also often reported thermophilic (Chen et al, 2023;Iino et al, 2010) increased at 40 °C while the Rhodospirillales and Burkholderiales, genus Denitratisoma, associated with facultatively anaerobic or even aerobic conditions decreased (Huang et al, 2019;Pfennig and Trüper, 2019;Ribeiro et al, 2022) further supporting that an excess of DO was not the major contributing factor to abatement of anammox bacteria.…”

Section: Changes In Microbial Communitymentioning

confidence: 89%

Mechanisms of Anammox Adaptation to High Temperatures: Increased Cyclization of Ladderane Lipids and Proteomic Insights

Christina,

Klára,

Adam

et al. 2024

Preprint

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Although anammox-based processes have been widely applied in mesophilic conditions of reject water and recently in mainstream conditions, the potential of their implementation in high-temperature wastewaters remains largely unexplored. Therefore, this study investigated the operation parameters for the successful adaptation of anammox bacteria and the mechanisms involved on the proteomic and cellular level including unique ladderane lipids. For this purpose, the enrichment of Candidatus Brocadia was cultivated in two fed-batch reactors (FBRs) at a lab scale. The temperature of one FBR was gradually increased from 30 to 40 degrees C while the other FBR was maintained at 30 degrees C with four consecutive replicates of this experiment. For this adaptation to be successful, the original loading rate had to be at least halved, or ideally maintained below half the value of the specific anammox activity at the time. The most notable adaptation mechanisms included: (1) upregulation of chaperones and (2) doubled ladderane cyclization via the replacement of non-ladderane fatty acid by a ladderane fatty acid in ladderane lipids (p-value 0.005). To our best knowledge, this is the first study to describe the novel mechanism of ladderane cyclization which together with other adaptation strategies presents crucial indicators in anammox adaptation to high-temperature wastewaters.

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Section: Changes In Microbial Communitymentioning

confidence: 89%

Mechanisms of Anammox Adaptation to High Temperatures: Increased Cyclization of Ladderane Lipids and Proteomic Insights

Christina,

Klára,

Adam

et al. 2024

Preprint

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“…In Stage III, the most dominant phyla were Proteobacteria (64%), Chloroflexi (12%), and Planctomycetes (7.1%), followed by Chlamydiae (2.6%), Actinobacteria (1.7%), Bacteroidetes (1.4%), Chlorobi (1.4%), Acidobacteria (1.1%), and Firmicutes (1.1%). Other studies using anammox-based reactors usually describe comparable microbial groups in relation to the dominant phyla [41]. Chloroflexi often occurs in anammox reactors without organic carbon compounds [42].…”

Section: The Microbial Community In the Context Of The Technological ...mentioning

confidence: 99%

Decrease in Oxygen Concentration for the Fast Start-Up of Partial Nitritation/Anammox without Inoculum Addition

Borowska,

Zubrowska-Sudol,

Doskocz

2024

Sustainability

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Initiating the partial nitritation and anammox (PN/A) process without inoculation poses a significant challenge. Thus, there is a notable amount of interest in devising a straightforward strategy for the start-up of PN/A. This study demonstrates the feasibility of achieving the rapid start-up of a one-stage PN/A process within a moving-bed sequencing batch biofilm reactor (MBSBBR) by reducing the oxygen concentrations: 3.0 mg O2/L (Stage I), 2.0 mg O2/L (Stage II), and 1.0 mg O2/L (Stage III). The anammox activity was observed 15 days after a gradual decrease in the oxygen concentration and confirmed using a specific anammox activity test (5.9 mg N/gVSS∙h). During Stage III, the average total inorganic nitrogen (TIN) removal efficiency was 60.6%. The relative abundance of planctomycetes, a typical phylum representing anammox microorganisms, increased almost three times from Stage I (2.8%) to Stage III (7.1%). These results demonstrate the potential of a decrease in oxygen concentration for the fast start-up of the one-stage partial nitritation and anammox process without inoculation. The implementation of the studied DO strategy has practical implications for wastewater treatment plant operators, particularly in the start-up of the PN/A processes. Additionally, batch assays allow for the rapid assessment of treatment plant performance, providing real-time insights into its functionality and, thereby, optimizing wastewater treatment practices.

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“…Ionized ammonia can be adsorbed from solution by cation exchange reactions with detritus, inorganic deposits, or soil. The adsorbed ammonia is loosely bound to the substrate and is easily released when the hydrochemical conditions change (Ribeiro et al, 2022). At a given concentration of ammonia in the water column, a certain amount of ammonia is adsorbed and saturated at the available binding sites.…”

Section: Nutrient Contentmentioning

confidence: 99%

Treatment Ability and Community Responses of Candung as an Appropriate Technology to Maintain Irrigation Water Quality

Sumantra¹,

Wijaya²,

Widnyana³

et al. 2023

J. Ecol. Eng.

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With the burgeoning population, the community activities, such as laundering, washing, or even bathing, have expanded along the irrigation canal. Consequently, some chemicals from those activities are released and pollute the water body. Besides, the discharge of wastewater into the irrigation system is also an issue of public health concern, because it creates an environment conducive to the reproduction of any pathogenic agent. Using candung as constructed wetlands (CW)-like to treat the irrigation water in rice paddies would be an interesting tool for removing pollutants from the irrigation water. Candung, as a locally used CW in Bali Island, has been applied in the rice field long time ago. The present study aimed to examine candung as a local attached CW or treating irrigation water and analyse the community awareness regarding the candung application. The result showed a positive effect of candung in maintaining the water quality parameters, such as TSS, BOD, COD, and nutrients with certain aquatic plants (Nelumbo nucifera, Nymphaea tetragona, and Pistia stratiotes). The knowledge, behaviour, and action of the local subak community were still limited, whereas they had positive perceptions and responses about candungthe potential of candung as an agrotourism attraction in the village as well.

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Microbial community composition, dynamics, and biogeochemistry during the start-up of a partial nitritation-anammox pathway in an upflow reactor

Cited by 8 publications

References 41 publications

Mechanisms of Anammox Adaptation to High Temperatures: Increased Cyclization of Ladderane Lipids and Proteomic Insights

Mechanisms of Anammox Adaptation to High Temperatures: Increased Cyclization of Ladderane Lipids and Proteomic Insights

Decrease in Oxygen Concentration for the Fast Start-Up of Partial Nitritation/Anammox without Inoculum Addition

Treatment Ability and Community Responses of Candung as an Appropriate Technology to Maintain Irrigation Water Quality

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