Microbial Community Shifts during Biogas Production from Biowaste and/or Propionate

Abstract

Propionate is the most delicate intermediate during anaerobic digestion as its degradation is thermodynamically unfavorable. To determine its maximum possible degradation rates during anaerobic digestion, a reactor was fed Monday to Friday with an organic loading rate (OLR) of 12/14 kg CODbiowaste·m−3·d−1 plus propionate up to a final OLR of 18 kg COD·m−3·d−1. No feed was supplied on weekends as it was the case in full-scale. To maintain permanently high propionate oxidizing activity (POA), a basic OLR of 3 kg CODpropionate·m−3·d−1 all week + 11 kg CODbiowaste·m−3·d−1 from Monday to Friday was supplied. Finally a reactor was operated with an OLR of 12 kg CODbiowaste·m−3·d−1 from Monday to Friday and 5 kg CODpropionate·m−3·d−1 from Friday night to Monday morning to maintain a constant gas production for permanent operation of a gas engine. The propionate degradation rates (PDRs) were determined for biowaste + propionate feeding. Decreasing PDRs during starvation were analyzed. The POA was higher after propionate supply than after biowaste feeding and decreased faster during starvation of a propionate-fed rather than a biowaste-fed inoculum. Shifts of the propionate-oxidizing and methanogenic community were determined.

Modification of plastic tank for bio-digestion of food wastes for biogas generation for cooking foods

“…Similar result was reported by Asikong et al (2016). This may be due to deposition of microbial metabolites and gradual exhaustion of nutrient from the wastes (Ziemiński and Frąc, 2012;Li et al, 2015;Asikong et al, 2016).…”

Response of Microbial Community to Induced Failure of Anaerobic Digesters Through Overloading With Propionic Acid Followed by Process Recovery

“…and Methanoculleus sp. was also observed (Shigematsu et al, 2006;Li et al, 2014;Li C. et al, 2015). As discussed by Hori et al (2006) Methanoculleus sp.…”

Molecular Detection of Biogenic Bacteria During Biogas Production Using Domestic Feed Stock

“…Study of the microbial community is very crucial for the optimization, control, and in-depth knowledge of the performance of the process for a better large scale biogas production. Recent developments in molecular biology techniques such as metagenomics approaches have provided a valuable tool for improved understanding of this complex microbiological system, which in turn could help optimize and control the process in an effective way (Hovarth et al, 2016;Li et al, 2015;Wirth et al, 2012 (Klocke et al, 2007;Mc Hugh et al, 2003), but the use of the mcrA gene, which codes for one of the key enzymes in methanogenesis, the αsubunit of methyl-coenzyme M reductase occurring uniquely in methanogens has greatly improved the understanding of the microbial community involved in anaerobic biogas production (Schlüter, et al, 2013;Krause et al, 2008;Kröber et al, 2009). Therefore, the general objective of this work was to identify the presence of methanogenic bacteria in a short-term operated biogas reactor fed with kitchen waste by PCR-amplification of the methyl coenzyme M reductase (mcrA).…”