Microbial community structure during oxygen-stimulated bioremediation in phenol-contaminated groundwater

Lin, Chi‐Wen; Lai, Chi-Yung; Chen, Li-Hsuan; Chiang, Wan-Fu

doi:10.1016/j.jhazmat.2006.06.083

Cited by 20 publications

(11 citation statements)

References 18 publications

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“…SSCP has been applied to study microbial communities in different matrices ranging from water (Lee et al, 1996;Lin et al, 2007) and soils (Sliwinski and Goodman, 2004;Witzig et al, 2006) to anaerobic digestors (Zumstein et al, 2000;Leclerc et al, 2001). However, so far applications in food microbiology have been limited to cheese (Duthoit et al, 2003;Delbè s et al, 2007).…”

Section: Single-strand Conformation Polymorphismmentioning

confidence: 99%

Recent advances in molecular techniques to study microbial communities in food-associated matrices and processes

2008

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Section: Single-strand Conformation Polymorphismmentioning

confidence: 99%

Recent advances in molecular techniques to study microbial communities in food-associated matrices and processes

2008

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“…A PCR-SSCP procedure by Lin et al (2007) was followed with a slight modification (time and number of cycles) in the PCR conditions. The entire procedure is described below.…”

Section: Bacterial Community Examinationmentioning

confidence: 99%

In situ Groundwater Remediation Using Enricher Reactor – Permeable Reactive Biobarrier

Kasi¹,

McEvoy²,

Padmanabhan³

et al. 2010

proc water environ fed

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Permeable reactive biobarrier (PRBB) is a flow-through zone where microorganisms degrade contaminants in groundwater. Discontinuous presence of contaminants in groundwater causes performance loss of a PRBB in removing the target contaminant. A novel enricher reactor (ER) -PRBB system was developed to treat groundwater with contaminants that reappear after an absence period. ER is an offline reactor for enriching contaminant degraders, which were used for augmenting PRBB to maintain its performance after a period of contaminant absence. The ER-PRBB concept was initially applied to remove benzene that reappeared after absence periods of 10 and 25 days. PRBBs without ER augmentation experienced performance losses of up to 15% higher than ER-PRBBs.The role of inducer compounds in the ER to enrich bacteria that can degrade a mixture of benzene, toluene, ethylbenzene, and xylene (BTEX) was investigated with an objective to minimize the use of toxic chemicals as inducers. Three inducer types were studied: individual BTEX compounds, BTEX mixture, and benzoate (a non toxic and a common intermediate for BTEX biodegradation). Complete BTEX removal was observed for degraders enriched on all three inducer types; however, the removal rates were dependent on the inducer type. Degraders enriched on toluene and BTEX had the highest degradation rates for BTEX of 0.006 to 0.014 day -1 and 0.006 to 0.012 day -1 , respectively, while degraders enriched on benzoate showed the lowest degradation rates of 0.004 to 0.009 day -1 .The ER-PRBB technique was finally applied to address the performance loss of a PRBB due to inhibition interactions among BTEX, when the mixture reappeared after a iv 10 day absence period. The ER-PRBBs experienced minimal to no performance loss, while PRBBs without ER augmentation experienced performance losses between 11% and 35%. Presence of ethanol during the BTEX absence period increased the performance loss of PRBB for benzene removal. PRBBs augmented with degraders enriched on toluene alone overcame the inhibition interaction between benzene and toluene indicating that toluene can be used as a single effective inducer in an ER. The ER-PRBB was demonstrated to be a promising remediation technique and has potential for applications to a wide range of organic contaminants.v ACKNOWLEDGEMENTS

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“…A Hoefer SE600 vertical gel electrophoresis apparatus was used for SSCP analysis in 10% polyacrylamide gel for 6 h at a constant voltage of 300 V. The gel temperature was maintained at 4°C by using a circulating water bath. Details of this procedure can be found in the protocol described by Lin et al [16].…”

Section: Pollutants and Water Analysismentioning

confidence: 99%

“…Similarities between microbial groups were calculated as Dice indices according to procedures appearing in previous reports [16,24]. Dendrograms were calculated by using a clustering algorithm of a UPGMA using cluster analysis of similarity indices, constructed by NTSYSpc Version 2.1e software (Exeter Software, USA).…”

Section: Pollutants and Water Analysismentioning

confidence: 99%

See 1 more Smart Citation

Microbial communities and biodegradation in lab-scale BTEX-contaminated groundwater remediation using an oxygen-releasing reactive barrier

Lin

Chen

Yet-Pole

et al. 2009

Bioprocess Biosyst Eng

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To remediate benzene, toluene, ethylbenzene and xylene (BTEX) -contaminated groundwater, a biotreatment process including biostimulation and bioaugmentation was simulated using oxygen-releasing reactive barriers (ORRB) and water with added BTEX in a lab-scale system. The results showed that the capability for BTEX removal decreases in the order of benzene, toluene, p-xylene, ethylbenzene for both added-nitrogen and noadded-nitrogen under BTEX concentrations at 30 mg l -1 . The removal efficiencies in ORRB systems were higher in the nitrogen-added condition for biostimulation compared with the no-nitrogen-added condition; moreover, an increased pattern for removal was observed during the bioaugmentation process. The oxygen content was found to be inversely proportional to the distance from the ORRB, as evidenced by observing that the average bacteria densities were two orders higher when located at 15 cm compared with 30 cm from the ORRB. The microbial community structure was similar in both cases of addednitrogen and the no-added-nitrogen conditions.

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Microbial community structure during oxygen-stimulated bioremediation in phenol-contaminated groundwater

Cited by 20 publications

References 18 publications

Recent advances in molecular techniques to study microbial communities in food-associated matrices and processes

Recent advances in molecular techniques to study microbial communities in food-associated matrices and processes

In situ Groundwater Remediation Using Enricher Reactor – Permeable Reactive Biobarrier

Microbial communities and biodegradation in lab-scale BTEX-contaminated groundwater remediation using an oxygen-releasing reactive barrier

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