Microbial community structure in autotrophic nitrifying granules characterized by experimental and simulation analyses

Matsumoto, Shimpei; Katoku, Mayu; Saeki, Goro; Terada, Akihiko; Aoi, Yoshiteru; Tsuneda, Satoshi; Picioreanu, Cristian; Loosdrecht, M.C.M. van

doi:10.1111/j.1462-2920.2009.02060.x

Cited by 108 publications

(72 citation statements)

References 65 publications

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“…In general, members of the genus Nitrosospira and the N. oligotropha clusters prevail in environments with low ammonia concentrations (Bollmann and Laanbroek 2001;Limpiyakorn et al 2005;Otawa et al 2006;Wang et al 2010), whereas environments rich in ammonia are dominated by members of the N. europaea-N. eutropha clusters (Logemann et al 1998;Limpiyakorn et al 2005;Tan et al 2008). Hence, the finding that the dominant AOB in the investigated nitrifying reactors belonged to the N. europaea lineage is not surprising and corresponds to what was observed before (Logemann et al 1998;Tan et al 2008;Matsumoto et al 2010). However, the peculiar morphology has no analogues among the so far described pure cultures of this AOB genus despite of being a well-documented phenomenon in the wastewater treatment plants and bioreactors.…”

Section: Discussionsupporting

confidence: 52%

Analysis of ammonia-oxidizing bacteria dominating in lab-scale bioreactors with high ammonium bicarbonate loading

Vejmelková

Sorokin

Abbas

et al. 2011

Appl Microbiol Biotechnol

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The ammonia-oxidizing bacterial community (AOB) was investigated in two types of laboratory-scale bioreactors performing partial oxidation of ammonia to nitrite or nitrate at high (80 mM) to extremely high (428 mM) concentrations of ammonium bicarbonate. At all conditions, the dominant AOB was affiliated to the Nitrosomonas europaea lineage as was determined by fluorescence in situ hybridization and polymerase chain reaction in combination with denaturing gradient gel electrophoresis. Molecular analysis of the mixed populations, based on the 16S rRNA and cbbL genes, demonstrated the presence of two different phylotypes of Nitrosomonas, while microbiological analysis produced a single phylotype, represented by three different morphotypes. One of the most striking features of the AOB populations encountered in the bioreactors was the domination of highly aggregated obligate microaerophilic Nitrosomonas, with unusual cellular and colony morphology, commonly observed in nitrifying bioreactors but rarely investigated by cultural methods. The latter is probably not an adaptation to stressful conditions created by high ammonia or nitrite concentrations, but oxygen seems to be a stressful factor in these bioreactors.

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Section: Discussionsupporting

confidence: 52%

Analysis of ammonia-oxidizing bacteria dominating in lab-scale bioreactors with high ammonium bicarbonate loading

Vejmelková

Sorokin

Abbas

et al. 2011

Appl Microbiol Biotechnol

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“…They were detected in wastewater treatment systems from different industries as well as in activated sludge systems in municipal WWTPs [21,22] and also in autotrophic nitrifying granules [23]. In the present study they appear almost in constant amounts in the reactors fed with synthetic media and fish cannery wastewater.…”

Section: Inoculum and Wastewater Compositionmentioning

confidence: 51%

Filamentous bacteria existence in aerobic granular reactors

Figueroa

Río

Campos

et al. 2014

Bioprocess Biosyst Eng

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Filamentous bacteria are associated to biomass settling problems in wastewater treatment plants. In systems based on aerobic granular biomass they have been proposed to contribute to the initial biomass aggregation process. However their development on mature aerobic granular systems has not been sufficiently studied. In the present research work filamentous bacteria were studied for the first time after long term operation (up to 300 days) of aerobic granular systems. Chloroflexi and Sphaerotilus natans have been observed in a reactor fed with synthetic wastewater. These filamentous bacteria could only come from the inoculated sludge. Thiothrix and Chloroflexi bacteria were observed in aerobic granular biomass treating wastewater from a fish canning industry. Meganema perideroedes was detected in a reactor treating wastewater from a plant processing marine products. As a conclusion the source of filamentous bacteria in these mature aerobic granular systems fed with industrial effluents was the incoming wastewater.

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“…5A). It has been reported that dissolved oxygen penetrates various nitrifying biofilms approximately 50-200 µm from the surface (17,27,31,38). It is therefore likely that the full penetration of the nascent granules by oxygen resulted in a uniform distribution of N. mobilis.…”

Section: Discussionmentioning

confidence: 99%

“…Matsumoto et al (27) reported that the inside of nitrifying granules 1.6 mm in diameter is anoxic and mainly occupied by heterotophic bacteria. The report concluded that the heterotophic bacteria conduct denitrification inside the nitrifying granules.…”

Section: No2mentioning

confidence: 99%

“…Even though the process of granulation has been evaluated extensively and several factors, such as the addition of Fe and shear stress caused by aeration (43), affecting the formation of nitrifying granules have been reported, understanding how the granules form and become stable remains a major challenge. There are several reports based on information obtained from mature granules (27,41), however, variations in the abundance and spatial distribution of microbial populations in developing nitrifying granules have not been evaluated extensively.…”

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Microbial Population Dynamics and Community Structure during the Formation of Nitrifying Granules to Treat Ammonia-Rich Inorganic Wastewater

et al. 2010

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Microbial population dynamics were investigated during the formation of nitrifying granules in an aerobic upflow fluidized bed (AUFB) reactor fed ammonia as a sole energy source. Analyses of clone libraries of 16S rRNA gene and the ammonia monooxygenase subunit A gene (amoA) revealed that although the clones obtained from the seed sludge were widely distributed among the ammonia-oxidizing bacteria (AOB) isolates, the community structure of AOB shifted towards the Nitrosomonas mobilis lineage as granulation proceeded. Quantitative fluorescence in situ hybridization showed that changes in the bacterial population occurred concomitantly with changes in nitrification performance and the size of granules. AOB associated with the N. mobilis lineage were predominant in the early stages as nitrifying granules formed (average diameter, 126 µm). In mature granules (average diameter, 270 µm), at least three types of AOB, N. mobilis, Nitrosomonas oligotropha, and Nitrosomonas europaea, formed different niches and coexisted. Nitrite-oxidizing bacteria (NOB) affiliated with Nitrospira spp. were detected in the start-up period, but were replaced by NOB affiliated with Nitrobacter spp. after granules formed.Key words: community structure, population dynamics, nitrifying bacteriaThe removal of biological nitrogen from various types of wastewater is becoming increasingly important owing to the implementation of strict regulations concerning nitrogen discharge. The process of nitrification involves the conversion of ammonia (NH3) or ammonium ions (NH4. Two types of nitrifying bacteria, namely, lithoautotrophic ammonia-oxidizing bacteria (AOB) and lithoautotrophic nitrite-oxidizing bacteria (NOB), play important roles in this process. AOB are classified into two phylogenetic lineages based on their 16S rRNA gene sequences; Nitrosomonas sp. and Nitrosospira sp. belonging to Betaproteobacteria, and Nitrosococcus oceani and Nitrosococcus halophilus belonging to Gammaproteobacteria (19). NOB are mainly classified into two phylogenetic lineages based on the cell morphology of isolated organisms and on 16S rRNA gene sequences. The most intensively studied NOB, Nitrobacter and Nitrospira, fall within the Alphaproeobacteria and separate phyla in Bacteria, respectively (2).It is generally accepted that nitrifying bacteria have a low growth rate and tend to be washed out from reactors. Thus, it is difficult to retain large numbers of them within a reactor. Therefore, there is a need for a simple yet effective method of immobilizing nitrifying bacteria within a reactor. Nitrifying granule production is one method of immobilization used to preserve a large population of nitrifying bacteria within a reactor (12,(40)(41)(42)(43). Tsuneda et al. (41) produced nitrifying granules in inorganic wastewater using an aerobic upflow fluidized bed (AUFB) reactor. These granules exhibited a good settling ability and a high rate of ammonia removal (1.5 kg-N m −3 d −1 ). Therefore, nitrifying granule production is a promising method for the effective remov...

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Microbial community structure in autotrophic nitrifying granules characterized by experimental and simulation analyses

Cited by 108 publications

References 65 publications

Analysis of ammonia-oxidizing bacteria dominating in lab-scale bioreactors with high ammonium bicarbonate loading

Analysis of ammonia-oxidizing bacteria dominating in lab-scale bioreactors with high ammonium bicarbonate loading

Filamentous bacteria existence in aerobic granular reactors

Microbial Population Dynamics and Community Structure during the Formation of Nitrifying Granules to Treat Ammonia-Rich Inorganic Wastewater

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