Microbial Concrete as a Sustainable Option for Infrastructural Development in Emerging Economies

Mondal, Sandip; Ghosh, Aparna

doi:10.1061/9780784482032.042

Cited by 10 publications

(7 citation statements)

References 37 publications

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“…For all strains, CaCl 2 enrichment of the MB culture medium led to significantly higher CaCO 3 production compared to unenriched MB. Indeed, an increase in dissolved calcium concentration in the medium is known to increase bacterial CaCO 3 production [84]. Nevertheless, for the same dissolved calcium concentration, isolated biocalcifying strains did not produce the same amount of CaCO 3 (Figure 6).…”

Section: Analysis Of Biocalcification Capacitymentioning

confidence: 95%

New Biocalcifying Marine Bacterial Strains Isolated from Calcareous Deposits and Immediate Surroundings

et al. 2021

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Marine bacterial biomineralisation by CaCO3 precipitation provides natural limestone structures, like beachrocks and stromatolites. Calcareous deposits can also be abiotically formed in seawater at the surface of steel grids under cathodic polarisation. In this work, we showed that this mineral-rich alkaline environment harbours bacteria belonging to different genera able to induce CaCO3 precipitation. We previously isolated 14 biocalcifying marine bacteria from electrochemically formed calcareous deposits and their immediate environment. By microscopy and µ-Raman spectroscopy, these bacterial strains were shown to produce calcite-type CaCO3. Identification by 16S rDNA sequencing provided between 98.5 and 100% identity with genera Pseudoalteromonas, Pseudidiomarina, Epibacterium, Virgibacillus, Planococcus, and Bhargavaea. All 14 strains produced carbonic anhydrase, and six were urease positive. Both proteins are major enzymes involved in the biocalcification process. However, this does not preclude that one or more other metabolisms could also be involved in the process. In the presence of urea, Virgibacillus halodenitrificans CD6 exhibited the most efficient precipitation of CaCO3. However, the urease pathway has the disadvantage of producing ammonia, a toxic molecule. We showed herein that different marine bacteria could induce CaCO3 precipitation without urea. These bacteria could then be used for eco-friendly applications, e.g., the formation of bio-cements to strengthen dikes and delay coastal erosion.

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Section: Analysis Of Biocalcification Capacitymentioning

confidence: 95%

New Biocalcifying Marine Bacterial Strains Isolated from Calcareous Deposits and Immediate Surroundings

et al. 2021

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“…Die MICP bietet unterschiedliche Anwendungsmo ¨glichkeiten. So konnten verschiedene Studien zeigen, dass sie das Potenzial hat, bestehende Baumaterialien wie Zementmo ¨rtel und Sandstein in ihrer Festigkeit und Wasserpermeabilita ¨t zu verbessern [42], Risse in Baumaterialien zu reparieren [43], und Materialien zu produzieren, die eine nachhaltigere und o ¨kologischere Alternative zu herko ¨mmlichen Baumaterialien, wie Beton, darstellen ko ¨nnten [44]. Der am ha ¨ufigsten genutzte Mechanismus der MICP ist die Fa ¨llung von Calciumcarbonat durch ureolytische Bakterien [45]…”

Section: Nachhaltig Bauen Mit Mikroorganismenunclassified

Nachhaltigkeit in der Bioverfahrenstechnik

Strieth

2022

Chemie Ingenieur Technik

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In this article, young scientist Dr.-Ing. Dorina Strieth from the Chair of Bioprocess Engineering at the TU Kaiserslautern introduces herself. In addition to current research work and teaching activities, she reports on the need for knowledge transfer to civil society. On a scientific level, she presents recent results on the intelligent use of phototrophic biofilms as well as the potential for biotechnological production of sustainable building materials.

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“…The production of novel building materials by MICP has also been described in various studies [7][8][9][10]. The mechanism most commonly used for this purpose is ureolysis [11,12].…”

Section: Introductionmentioning

confidence: 99%

“…Past studies have already shown that MICP can be used to increase strength [ 3 , 4 ] and repair cracks in concrete and sandstone [ 5 , 6 ]. The production of novel building materials by MICP has also been described in various studies [ 7 , 8 , 9 , 10 ]. The mechanism most commonly used for this purpose is ureolysis [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Sporosarcina pasteuriican be used to print a layer of calcium carbonate

Erdmann

Kästner

Payrebrune

et al. 2022

Engineering in Life Sciences

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When using microbiologically induced calcium carbonate precipitation (MICP) to produce calcium carbonate crystals in the cavities between mineral particles to consolidate them, the inhomogeneous distribution of the precipitated calcium carbonate poses a problem for the production of construction materials with consistent parameters. Various approaches have been investigated in the literature to increase the homogeneity of consolidated samples. One approach can be the targeted application of ureolytic organisms by 3D printing. However, to date, this possibility has been little explored in the literature. In this study, the potential to use MICP to print calcium carbonate layers on mineral particles will be investigated. For this purpose, a dispensing unit was modified to apply both a suspension of Sporosarcina pasteurii and a calcination solution containing urea and calcium chloride onto quartz sand. The study showed that after passing through the nozzle, S. pasteurii preserved consistent cell vitality and therefore its potential of MICP. Applying cell suspension and calcination solution through a printing nozzle resulted in a layer of calcium carbonate crystals on quartz sand. This observation demonstrated the proof of concept of printing calcium carbonate by MICP through the nozzle of a dispensing unit. Furthermore, it was shown that cell suspensions of S. pasteurii can be stored at 4°C for a period of 17 days while maintaining its optical density, urease activity and cell vitality and therefore the potential for MICP. This initial concept could be extended in further research to printing three‐dimensional (3D) objects to solve the problem of homogeneity in consolidated mineral particles.

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Microbial Concrete as a Sustainable Option for Infrastructural Development in Emerging Economies

Cited by 10 publications

References 37 publications

New Biocalcifying Marine Bacterial Strains Isolated from Calcareous Deposits and Immediate Surroundings

New Biocalcifying Marine Bacterial Strains Isolated from Calcareous Deposits and Immediate Surroundings

Nachhaltigkeit in der Bioverfahrenstechnik

Sporosarcina pasteuriican be used to print a layer of calcium carbonate

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