Microbial differences in the habitats of lithophytic bryophytes and their relationship with soil nutrients

Abstract

Background:Lithophytic Bryophyte is a pioneer plant on the exposed rock surface in karst area,and they can alter the microorganisms in the rock habitat. Results:At the species level, the number of species of various microorganisms in the habitat after the rocky moss was planted on the rock surface was Fungi 235, Bacteria 20535, Eukaryota 816, Archaea 503, and Viruses 155, respectively. Compared with the original control soil, the growth of various microorganisms was Fungi 52%, Bacteria 11%, Eukaryota 78%, Archaea 27%, and Viruses 146%. The number of microbial species related to carbon fixation was 2779, nitrogen fixation was 1502, phosphorus metabolism was 1750.Compared with the original control soil, the bryophytes increased by 37%, 49% and 53% respectively after planting the rock surface. Compared with the original soil, the exposed rock surface increased by 20%, nitrogen fixation by 28% and phosphorus metabolism by 31%.Microbial species with significant differences between groups,Acidimimicrobia_bacterium,Acidimimicrobiaceae_bacterium,Acidimimicrobiales_bacterium, Iamiaceae_bacterium_SCSIO_58843 is significantly positively correlated with potassium content in soil,Microcoleus_Sp._PCC_7113 is a significant negative correlated with potassium content in soil.Alphaprotoobjective_bacterium, Solirubrobacteriales_bacterium, Betaproteobjective_bacterium is a significant positive correlated with succinic acid content in soil.Chloroflexi_bacterium is a significant positive correlated with oxalic acid content insoil.Acidobacteria_bacterium,Solirubrobacterales_bacterium,Acidimicrobiaceae_bacterium is a significant negative correlated with malic acid in soil.Gemmatimonadetes_bacterium is a significant negative correlated with oxalic acid. Smaragdicoccus_niigatensis,Gemmatimonadetes_bacterium,Nocardiaceae_bacterium_YC2-7 is significantly negatively correlated with succinic acid in soil. Solirubrobacterales_bacterium,Archangium_gephyra is a significant negative correlated with acetic acid in soil. Conclusions:The lithophytic bryophytes changed the microbial composition structure in the rock surface habitat, significantly increased the number of functional microorganisms, and then increased the accumulation of potassium, phosphorus, organic carbon and malic acid in the habitat, and promoted the positive development of the rock surface ecosystem.