Microbial Diversity and Biochemical Potential Encoded by Thermal Spring Metagenomes Derived from the Kamchatka Peninsula

Wemheuer, Bernd; Taube, Robert; Akyol, Pinar; Wemheuer, Franziska; Daniel, Rolf

doi:10.1155/2013/136714

Cited by 79 publications

(58 citation statements)

References 62 publications

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“…OctSpA1-106 was originally detected at Octopus Spring in Yellowstone National Park (USA) 24 and has since been detected at El Tatio Geyser Field (Chile) 36 , Uzon Caldera (Russia) 37 and terrestrial hot-spring areas in North America 28,38 . It has been detected only in neutral to alkaline, geothermally heated environments.…”

Section: Discussionmentioning

confidence: 99%

Evidence of global-scale aeolian dispersal and endemism in isolated geothermal microbial communities of Antarctica

et al. 2014

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Section: Discussionmentioning

confidence: 99%

Evidence of global-scale aeolian dispersal and endemism in isolated geothermal microbial communities of Antarctica

et al. 2014

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“…The modification concerned the reclassification of sequences affiliated the Roseobacter OCT lineage, which in fact consists of members of the CHAB-I-5, NAC1-2 and NAC11-6 lineages. Rarefaction curves and alpha diversity indices were calculated with QIIME as descripted by Wemheuer et al (2013).…”

Section: Bc Compositionmentioning

confidence: 99%

Deciphering associations between dissolved organic molecules and bacterial communities in a pelagic marine system

et al. 2016

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Dissolved organic matter (DOM) is the main substrate and energy source for heterotrophic bacterioplankton. To understand the interactions between DOM and the bacterial community (BC), it is important to identify the key factors on both sides in detail, chemically distinct moieties in DOM and the various bacterial taxa. Next-generation sequencing facilitates the classification of millions of reads of environmental DNA and RNA amplicons and ultrahigh-resolution mass spectrometry yields up to 10 000 DOM molecular formulae in a marine water sample. Linking this detailed biological and chemical information is a crucial first step toward a mechanistic understanding of the role of microorganisms in the marine carbon cycle. In this study, we interpreted the complex microbiological and molecular information via a novel combination of multivariate statistics. We were able to reveal distinct relationships between the key factors of organic matter cycling along a latitudinal transect across the North Sea. Total BC and DOM composition were mainly driven by mixing of distinct water masses and presumably retain their respective terrigenous imprint on similar timescales on their way through the North Sea. The active microbial community, however, was rather influenced by local events and correlated with specific DOM molecular formulae indicative of compounds that are easily degradable. These trends were most pronounced on the highest resolved level, that is, operationally defined 'species', reflecting the functional diversity of microorganisms at high taxonomic resolution.

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“…In the Kamchatka peninsula, also known as the land of fire, an extended volcanic region of approximately 472,300 km 2 , three different areas, Uzon (81°C, pH 7.2-7.4), Kam37 (85°C, pH 5.5) and Mutnovsky (70°C, pH 3.5-4.0), were surveyed to study the diversity of their microbial communities (Chernyh et al 2015;Eme et al 2013;Merkel et al 2017;Wemheuer et al 2013). This analysis, besides showing that uncultivated members of the Aquificales, Euryarchaeota, Crenarchaeota, and a Miscellaneous Crenarchaeotic Group were dominating in Kam37, led also to the discovery of two ancient (hyper)thermophilic archaeal lineages, namely Hot Thaumarchaeota-related Clade 1 and Hot Thaumarchaeotarelated Clade 2.…”

Section: Kamchatka Peninsula Russiamentioning

confidence: 99%

Metagenomics of microbial and viral life in terrestrial geothermal environments

Strazzulli

Fusco

Cobucci‐Ponzano

et al. 2017

Rev Environ Sci Biotechnol

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Geothermally heated regions of Earth, such as terrestrial volcanic areas (fumaroles, hot springs, and geysers) and deep-sea hydrothermal vents, represent a variety of different environments populated by extremophilic archaeal and bacterial microorganisms. Since most of these microbes thriving in such harsh biotopes, they are often recalcitrant to cultivation; therefore, ecological, physiological and phylogenetic studies of these microbial populations have been hampered for a long time. More recently, culture-independent methodologies coupled with the fast development of next generation sequencing technologies as well as with the continuous advances in computational biology, have allowed the production of large amounts of metagenomic data. Specifically, these approaches have assessed the phylogenetic composition and functional potential of microbial consortia thriving within these habitats, shedding light on how extreme physico-chemical conditions and biological interactions have shaped such microbial communities. Metagenomics allowed to better understand that the exposure to an extreme range of selective pressures in such severe environments, accounts for genomic flexibility and metabolic versatility of microbial and viral communities, and makes extreme-and hyper-thermophiles suitable for bioprospecting purposes, representing an interesting source for novel thermostable proteins that can be potentially used in several industrial processes.Keywords Hyperthermophiles Á Geothermal environments Á Microbial and viral metagenomics Á CRISPR Á Enzyme discovery BackgroundHot springs populated by extreme thermophiles (T opt : 65-79°C) and hyperthermophiles (T opt [ 80°C) (DeCastro et al. 2016) are very diverse and some of them show combinations of extreme chemo-physical conditions, such as temperature, acidic or alkaline pHs, high pressure, and high concentrations of salts and heavy metals (López-López et al. 2013). As with all studies of environmental microbiology, our understanding of the composition, functional and physiological dynamics, and evolution of extreme-and hyper-thermophilic microbial consortia has lagged A. Strazzulli Á M. Moracci Á P. Contursi

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Microbial Diversity and Biochemical Potential Encoded by Thermal Spring Metagenomes Derived from the Kamchatka Peninsula

Cited by 79 publications

References 62 publications

Evidence of global-scale aeolian dispersal and endemism in isolated geothermal microbial communities of Antarctica

Evidence of global-scale aeolian dispersal and endemism in isolated geothermal microbial communities of Antarctica

Deciphering associations between dissolved organic molecules and bacterial communities in a pelagic marine system

Metagenomics of microbial and viral life in terrestrial geothermal environments

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