Microbial Diversity and Possible Activity in Nitrate- and Radionuclide-Contaminated Groundwater

Назина, Т. Н.; Бабич, Т. Л.; Kostryukova, N. K.; Соколова, Д. Ш.; Abdullin, Ruslan R.; Tourova, T. P.; Полтараус, А. Б.; Kalmykov, Stepan N.; Zakharova, Еlena V.; Myasoedov, Β. F.; Nagaosa, Kazuyo; Kato, Kenji

doi:10.1007/978-981-15-0679-6_2

Cited by 4 publications

(6 citation statements)

References 48 publications

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“…All physiological tests were carried out in triplicate and chemotaxonomic tests in duplicate. Growth at different temperatures (5,9,15,22,28,30,33,37, and 42 • C), salt tolerance (0, 0.1, 0.3, 0.5, 0.8, 1.2, 1.6, 2.0, 2.5, 3.0, 3.5, and 4.0% (w/v) NaCl) and pH range for growth (5.0-10.5 with increments of ~0.5 pH units) were determined on TEG medium for 3-5 days at aerobic conditions optimal for growth of the strains.…”

Section: Phenotypic Characterizationmentioning

confidence: 99%

“…Bacteria of the genera Ensifer and Sinorhizobium , capable of denitrification, can affect the mobility of variable-valency metals and radionuclides in groundwater contaminated with components of liquid radioactive waste in the areas of the nuclear fuel cycle enterprises. When liquid radioactive waste is buried in surface water bodies and underground layers, toxic components gradually seep into the groundwater [ 21 , 22 ]. Liquid radioactive wastes are enriched in nitrates and organic salts.…”

Section: Introductionmentioning

confidence: 99%

“…The microorganisms can also have an effect on radionuclides and heavy metals due to their immobilization in the exopolysaccharide matrix of bacterial biofilms [ 23 , 24 ], in the processes of biosorption and bioaccumulation on the surface or inside the cells [ 25 , 26 ], and in the processes of direct reduction of variable-valency elements to poorly soluble compounds during cellular respiration [ 22 , 24 , 27 , 28 , 29 ]. Thus, the study of microorganisms isolated from radionuclide-contaminated groundwater, as well as from oil reservoirs, has both basic and applied importance.…”

Section: Introductionmentioning

confidence: 99%

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Genome Analysis and Potential Ecological Functions of Members of the Genus Ensifer from Subsurface Environments and Description of Ensifer oleiphilus sp. nov.

Ershov,

Babich,

Grouzdev

et al. 2023

Microorganisms

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The current work deals with genomic analysis, possible ecological functions, and biotechnological potential of two bacterial strains, HO-A22T and SHC 2-14, isolated from unique subsurface environments, the Cheremukhovskoe oil field (Tatarstan, Russia) and nitrate- and radionuclide-contaminated groundwater (Tomsk region, Russia), respectively. New isolates were characterized using polyphasic taxonomy approaches and genomic analysis. The genomes of the strains HO-A22T and SHC 2-14 contain the genes involved in nitrate reduction, hydrocarbon degradation, extracellular polysaccharide synthesis, and heavy metal detoxification, confirming the potential for their application in various environmental biotechnologies. Genomic data were confirmed by cultivation studies. Both strains were found to be neutrophilic, chemoorganotrophic, facultatively anaerobic bacteria, growing at 15–33 °C and 0–1.6% NaCl (w/v). The 16S rRNA gene sequences of the strains were similar to those of the type strains of the genus Ensifer (99.0–100.0%). Nevertheless, genomic characteristics of strain HO-A22T were below the thresholds for species delineation: the calculated average nucleotide identity (ANI) values were 83.7–92.4% (<95%), and digital DNA–DNA hybridization (dDDH) values were within the range of 25.4–45.9% (<70%), which supported our conclusion that HO-A22T (=VKM B-3646T = KCTC 92427T) represented a novel species of the genus Ensifer, with the proposed name Ensifer oleiphilus sp. nov. Strain SHC 2-14 was assigned to the species ‘Ensifer canadensis’, which has not been validly published. This study expanded the knowledge about the phenotypic diversity among members of the genus Ensifer and its potential for the biotechnologies of oil recovery and radionuclide pollution treatment.

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Section: Phenotypic Characterizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome Analysis and Potential Ecological Functions of Members of the Genus Ensifer from Subsurface Environments and Description of Ensifer oleiphilus sp. nov.

Ershov,

Babich,

Grouzdev

et al. 2023

Microorganisms

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“…and 'Candidatus Pelagibacter sp.' with small-sized cells [21]. Over 50 strains of aerobic organotrophic and anaerobic denitrifying and iron-reducing bacteria belonging to 23 genera and 33 species were isolated from the samples.…”

Section: Introductionmentioning

confidence: 99%

Ultramicrobacteria from Nitrate- and Radionuclide-Contaminated Groundwater

et al. 2020

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The goal of the present work was to investigate the physicochemical and radiochemical conditions and the microbial diversity in groundwater collected near the Lake Karachai (Russia), which was formerly used for the disposal of liquid radioactive waste, to isolate the dominant bacteria, and to determine their taxonomy and the physiological characteristics responsible for their adaptation to this environment. Groundwater samples contained high concentrations of acetate, oxalate, nitrate, and sulfate, as well as radionuclides. High-throughput sequencing and analysis of the clone libraries revealed lower microbial diversity in the most strongly contaminated groundwater and a predominance of bacteria of the genera Polynucleobacter, Pusillimonas, Candidatus Pelagibacter, and of the candidate phylum Parcubacteria; these groups include species with an ultra small cell size. Archaeal sequences in the libraries belonged to ammonium oxidizers of the phylum Thaumarchaeota and methanogens of the phylum Euryarchaeota. Pure cultures of obligate and facultative ultramicrobacteria belonging to the genera Chryseobacterium, Microbacterium, Salinibacterium, Pusillimonas, Roseomonas, and Janibacter were isolated from water samples. In genomes of Pusillimonas and Roseomonas strains the genes associated with nitrate reduction, resistance to heavy metals and metalloids were revealed. Several isolates are able to participate in the geochemical process of nitrate conversion to N 2 using acetate; this results in decreasing redox potential, which in turn may stimulate radionuclide reduction and decrease radionuclide migration in groundwater.

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“…Patescibacteria have been suggested to be symbionts of other microbes [6][7][8][9] or even considered virus-like [10]. Because of the frequent lack of a respiratory chain [5,10] and their presence in oxygen-deprived and anaerobic ecosystems [11][12][13][14] a fermentative-based lifestyle has been suggested for most Cand. Patescibacteria [10].…”

Section: Introductionmentioning

confidence: 99%

Overlooked Diversity of Ultramicrobacterial Minorities at the Air-Sea Interface

et al. 2020

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Members of the Candidate phylum Patescibacteria, also called Candidate Phyla Radiation (CPR), are described as ultramicrobacteria with limited metabolic capacities. Wide diversity and relative abundances up to 80% in anaerobic habitats, e.g., in groundwater or sediments are characteristic for Candidatus Patescibacteria. However, only few studies exist for marine surface water. Here, we report the presence of 40 patescibacterial candidate clades at air-sea interfaces, including the upper water layer, floating foams and the sea-surface microlayer (SML), a < 1 mm layer at the boundary between ocean and atmosphere. Particle-associated (>3 µm) and free-living (3–0.2 µm) samples were obtained from the Jade Bay, North Sea, and 16S rRNA (gene) amplicons were analyzed. Although the abundance of Cand. Patescibacteria representatives were relatively low (<1.3%), members of Cand. Kaiserbacteria and Cand. Gracilibacteria were found in all samples. This suggests profound aerotolerant capacities of these phylogenetic lineages at the air-sea interface. The presence of ultramicrobacteria in the >3 µm fraction implies adhesion to bigger aggregates, potentially in anoxic niches, and a symbiotic lifestyle. Due to their small sizes, Cand. Patescibacteria likely become aerosolized to the atmosphere and dispersed to land with possible implications for affecting microbial communities and associated processes in these ecosystems.

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Microbial Diversity and Possible Activity in Nitrate- and Radionuclide-Contaminated Groundwater

Cited by 4 publications

References 48 publications

Genome Analysis and Potential Ecological Functions of Members of the Genus Ensifer from Subsurface Environments and Description of Ensifer oleiphilus sp. nov.

Genome Analysis and Potential Ecological Functions of Members of the Genus Ensifer from Subsurface Environments and Description of Ensifer oleiphilus sp. nov.

Ultramicrobacteria from Nitrate- and Radionuclide-Contaminated Groundwater

Overlooked Diversity of Ultramicrobacterial Minorities at the Air-Sea Interface

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