Microbial functional genes driving the positive priming effect in forest soils along an elevation gradient

Zhao, Fazhu; Wang, Jieying; Li, Yang; Xu, Xiaofeng; He, Liyuan; Wang, Jun; Ren, Chao; Guo, Yaoxing

doi:10.1016/j.soilbio.2021.108498

Cited by 39 publications

(9 citation statements)

References 61 publications

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“…There was no altitudinal pattern in REs on investigated soil assays (Figures 2-5). Two previous studies reported that the REs on soil C min (priming effects) decreased significantly with increased altitude in tropical and temperate forests [42,43]. However, our results were inconsistent with those of the previous studies.…”

Section: Altitudinal Patterns Of the Relative Rhizosphere Effectscontrasting

confidence: 99%

Rhizosphere Effects along an Altitudinal Gradient of the Changbai Mountain, China

Huo

Yin

et al. 2022

Forests

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Rhizosphere effects (REs) play important roles in regulating carbon (C) and nutrient cycling in terrestrial ecosystems. However, little is known about the REs of mature trees in the field, especially at the ecosystem scale. This study aimed to explore the variation and patterns of REs in natural ecosystems. Here, combining soil monoliths with an adhering soil (shaking fine roots) method was adopted to sample paired rhizosphere soil and bulk soil along an altitudinal gradient. Based on the relative REs and the percentage of rhizosphere soil mass, the REs on soil C and net nitrogen mineralization rates (Cmin and net Nmin) at the ecosystem scale were estimated. Our results showed that the REs on soil processes, soil microbial biomass C and extracellular enzyme activities (β-glucosidase and N-acetyl-glucosaminidase activities), and soil chemical properties (total C, total N, inorganic N, extractable P, K, Ca, Mg, Fe, and Mn) were significantly positive across altitudinal sites, while soil pH was significantly negative. Although the relative REs on investigated variables varied significantly among altitudes, the relative REs did not show a clear trend with the increased altitudes. Across altitudes, the mean magnitude of ecosystem-level REs on Cmin and net Nmin were 19% (ranging from 4% to 48%) and 16% (ranging from 3% to 34%), respectively. Furthermore, the magnitude of ecosystem-level rhizosphere effects increased linearly with the increased altitudes. The altitudinal patterns of ecosystem-level RE mainly depend on the percentage of rhizosphere soil mass. In conclusion, our results provided a set of new evidence for the REs, and highlighted the need to incorporate REs into land C and N models.

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Section: Altitudinal Patterns Of the Relative Rhizosphere Effectscontrasting

confidence: 99%

Rhizosphere Effects along an Altitudinal Gradient of the Changbai Mountain, China

Huo

Yin

et al. 2022

Forests

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“…The network thus generated was visualised using the interactive platform Gephi (Bastian et al, 2009). For generating the Chordal graph, we used the "circlize" package (Gu, 2013) in R v4.0.2, and PLS-PM plots were drawn using Adobe IIIustrator 2020 (Zhao et al, 2022). Other graphs were drawn using the "ggplot2" package in R v4.0.2.…”

Section: Discussionmentioning

confidence: 99%

“…How to cite this article: Wang, J., He, L., Xu, X., Ren, C., Wang, J., Guo, Y., & Zhao, F. (2022). Linkage between microbial functional genes and net N mineralisation in forest soils along an elevational gradient.…”

Section: Conflict Of Interestmentioning

confidence: 99%

“…In this regard, the findings of our recent work have highlighted the important role of functional genes encoding enzymes involved in stable carbon (C) degradation. Moreover, bacteria in the phylum Proteobacteria have been identified as key species driving C-cycling in response to C input along an elevational gradient (Zhao et al, 2022). However, despite compelling evidence indicating the important roles of microbial community composition and functional genes in determining N cycling, we still have only a relatively limited understanding of the means whereby these attributes contribute to promoting soil N mineralisation.…”

Section: Introductionmentioning

confidence: 99%

“…vegetation, and soil characteristics from the different study sites(Zhao et al, 2022) Note: Values are represented as the mean value followed by standard error in parentheses (n = 3). Significant P values are marked in bold.…”

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confidence: 99%

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Linkage between microbial functional genes and net N mineralisation in forest soils along an elevational gradient

Wang

et al. 2022

European J Soil Science

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Soil net nitrogen (N) mineralisation, the difference between organic nitrogen mineralisation and mineral nitrogen immobilisation, changes with elevation, thereby determining plant productivity and soil N cycling along elevation gradients. However, it has yet to be established how different microbial functional genes influence the rate of soil net N mineralisation along such gradients. To address this deficiency in our current knowledge, we performed metagenomic sequencing to identify soil microbial functional genes encoding enzymes involved in N cycling at five forest sites along an elevational gradient spanning a range from 1503 to 3182 m above sea level. Our results indicate that the rate of net N mineralisation follows a unimodal pattern with increasing elevation, with a peak (0.18 mg kg−1 d−1) being detected at the mid‐high elevation site. Further, we detected a significant correlation between the abundance of genes encoding enzymes involved in denitrification and ammonia assimilation pathways and net N mineralisation rate (p < 0.05). Moreover, we established that microbial species in the phyla Cyanobacteria, Acidobacteria and Planctomycetes, harbouring keystone functional genes, play a predominant role in determining the rate of net N mineralisation. Our findings also revealed soil substrate content (ammonium nitrogen and nitrite nitrogen, soil organic carbon, and C:N ratio) and soil environment (soil temperature and soil moisture) to be the major drivers of net N mineralisation in soil via their regulatory effects on the composition of microbial communities and functional genes. Our characterisation of the microbial metagenomic basis of net N mineralisation in forest soils accordingly highlights the importance of the combined contributions of soil microbial functional genes, soil substrate, and environmental factors in determining the cycling of N in forest soils at different elevations. Highlights Net N mineralisation rate followed a unimodal pattern along an elevational gradient. Denitrification and ammonia assimilation genes were correlated with net N mineralisation rate. Cyanobacteria play a dominant role in determining net N mineralisation rate. Soil substrate and environment determine microbial functional gene distribution.

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Soil types differ in the temporal response of the priming effect to nitrogen addition: a study on microbial mechanisms

et al. 2023

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Microbial functional genes driving the positive priming effect in forest soils along an elevation gradient

Cited by 39 publications

References 61 publications

Rhizosphere Effects along an Altitudinal Gradient of the Changbai Mountain, China

Rhizosphere Effects along an Altitudinal Gradient of the Changbai Mountain, China

Linkage between microbial functional genes and net N mineralisation in forest soils along an elevational gradient

Soil types differ in the temporal response of the priming effect to nitrogen addition: a study on microbial mechanisms

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