Microbial Genomics and the Periodic Table

Wackett, Lawrence P.; Dodge, Anthony G.; Ellis, Lynda B. M.

doi:10.1128/aem.70.2.647-655.2004

Cited by 147 publications

(94 citation statements)

References 145 publications

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“…The effect of lithium ions on B. abortus growth/metabolism has not, to our knowledge, been explored. However, it is established that lithium ion can substitute for sodium ion in cotransport of amino acids and some sugars (32).…”

Section: Resultsmentioning

confidence: 99%

Conserved ABC Transport System Regulated by the General Stress Response Pathways of Alpha- and Gammaproteobacteria

et al. 2017

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Brucella abortus E1 is an EcfG family sigma factor that regulates the transcription of dozens of genes in response to diverse stress conditions and is required for maintenance of chronic infection in a mouse model. A putative ATPbinding cassette transporter operon, bab1_0223-bab1_0226, is among the most highly activated gene sets in the E1 regulon. The proteins encoded by the operon resemble quaternary ammonium-compatible solute importers but are most similar in sequence to the broadly conserved YehZYXW system, which remains largely uncharacterized. Transcription of yehZYXW is activated by the general stress sigma factor S in Enterobacteriaceae, which suggests a functional role for this transport system in bacterial stress response across the classes Alphaproteobacteria and Gammaproteobacteria. We present evidence that B. abortus YehZYXW does not function as an importer of known compatible solutes under physiological conditions and does not contribute to the virulence defect of a E1 -null strain. The sole in vitro phenotype associated with genetic disruption of this putative transport system is reduced growth in the presence of high Li ϩ ion concentrations. A crystal structure of B. abortus YehZ revealed a class II periplasmic binding protein fold with significant structural homology to Archaeoglobus fulgidus ProX, which binds glycine betaine. However, the structure of the YehZ ligand-binding pocket is incompatible with high-affinity binding to glycine betaine. This is consistent with weak measured binding of YehZ to glycine betaine and related compatible solutes. We conclude that YehZYXW is a conserved, stress-regulated transport system that is phylogenetically and functionally distinct from quaternary ammonium-compatible solute importers.IMPORTANCE Brucella abortus E1 regulates transcription in response to stressors encountered in its mammalian host and is necessary for maintenance of chronic infection in a mouse model. The functions of the majority of genes regulated by E1 remain undefined. We present a functional/structural analysis of a conserved putative membrane transport system (YehZYXW) whose expression is strongly activated by E1 . Though annotated as a quaternary ammonium osmolyte uptake system, experimental physiological studies and measured ligand-binding properties of the periplasmic binding protein (PBP), YehZ, are inconsistent with this function. A crystal structure of B. abortus YehZ provides molecular insight into differences between bona fide quaternary ammonium osmolyte importers and YehZ-related proteins, which form a distinct phylogenetic and functional group of PBPs.

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Section: Resultsmentioning

confidence: 99%

Conserved ABC Transport System Regulated by the General Stress Response Pathways of Alpha- and Gammaproteobacteria

et al. 2017

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“…Remarkably, a Au(I)-C-complex was generated, suggesting that C. metallidurans might use methylation of Au as an additional detoxification mechanism. Methylation of metals, e.g., Hg, Bi, Tn, or Ge, can be effected by microorganisms (21,22) and has been proposed for Au (11). Methyl-Au contains a Au(I)-C bond (23), and its XANES spectrum is likely to be similar to that of the Au(I)-CN model compound.…”

Section: Si Materials and Methods)mentioning

confidence: 99%

Mechanisms of gold biomineralization in the bacterium Cupriavidus metallidurans

Reith

Etschmann

Große

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

295

274

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“…The metalloid arsenic (As) is a member of group V of the periodic table and is thus classified as a heavy metal [1]. Although arsenic is generally toxic to life, it has been demonstrated that microorganisms can use arsenic compounds as electron donors or electron acceptors, and that they can possess arsenic detoxification mechanisms [2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Arsenic accumulation by Pseudomonas stutzeri and its response to some thiol chelators

Joshi

Patel

Flora

et al. 2008

Environ Health Prev Med

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Objective The aim of this study is to examine arsenic accumulation by Pseudomonas stutzeri and its response to some thiol chelators, DMPS and MiADMSA. MethodsDetermination of arsenic accumulation by Pseudomonas sp. was carried out using an atomic absorption spectrophotometer, a TEM and an EDAX. Arsenate reductase enzyme assay was carried out from a cell-free extract of Pseudomonas sp. The effect of chelating agents on arsenite accumulation was analyzed. Total cellular proteins were analyzed using 1-D SDS-PAGE. Results Pseudomonas sp. exhibited a maximum accumulation of 4 mg As g -1 (dry weight). TEM and EDAX analysis showed the presence of As-containing electrondense particles inside the cells. Data on arsenate reductase enzyme kinetics yielded a K m of 0.40 mM for arsenate and a V max of 5,952 lmol arsenate reduced per minute per milligram of protein. The chelating agents MiADMSA and DMPS were found to reduce the arsenic accumulation by 60 and 35%, respectively, whereas the presence of both chelating agents in medium containing cells pretreated with arsenite reduced it by up to 90%. The total protein profile of the cellular extract, obtained by 1-D SDS-PAGE, indicated five upregulated proteins, and three of these proteins exhibited differential expression when the cells were grown with MiADMSA and DMPS. Conclusion This study shows a new approach towards arsenic detoxification. A combination treatment with MiADMSA and DMPS may be useful for removing intracellular arsenic. The proteins that were found to be induced in this study may play an important role in the extrusion of arsenic from the cells, and this requires further characterization.

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Microbial Genomics and the Periodic Table

Cited by 147 publications

References 145 publications

Conserved ABC Transport System Regulated by the General Stress Response Pathways of Alpha- and Gammaproteobacteria

Conserved ABC Transport System Regulated by the General Stress Response Pathways of Alpha- and Gammaproteobacteria

Mechanisms of gold biomineralization in the bacterium Cupriavidus metallidurans

Arsenic accumulation by Pseudomonas stutzeri and its response to some thiol chelators

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