Microbial Isolation and Characterization of New Antibiotic-Producing Strains from Decayed Wood

Characterization of Microbial Diversity in Decayed Wood from a Spanish Forest: An Environmental Source of Industrially Relevant Microorganisms

“…Wood pieces for microbial isolation studies were harvested and processed following the protocol previously described by Velasco-Rodríguez et al in 2021 [39]. Supplementary Table S1 lists all the collected samples.…”

“…Tests were performed to determine the antifungal activity of the isolated strains, as previously described by Velasco-Rodríguez et al in 2021 [39]. In brief, the isolated Actinobacteria and Penicillium strains were seeded on both sides of the reporter species, which were five wellknown wood-decaying fungi representing the different types of wood damage: Aspergillus brasiliensis and Penicillium chrysogenum (moulds with some soft rot ability), Coniophora puteana (brown rot), Trametes versicolor (white rot), and Aureobasidium pullulans (blue stain) [7,46].…”

“…The bioassay was evaluated by comparing the growth of the reporter species alone versus the growth when a microbial antagonist candidate was present. The intensity of the inhibition was scored from 0 to 2, where "0" represents no inhibitory effect, "1" is a partial effect, and "2" represents a total inhibition of the reporter fungi [39].…”