Microbial lipids from organic wastes: Outlook and challenges

Tomás‐Pejó, Elia; Morales-Palomo, S.; González‐Fernández, Cristina

doi:10.1016/j.biortech.2020.124612

Cited by 58 publications

(26 citation statements)

References 140 publications

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“…Interestingly, aromatic compound metabolism can be linked to biodiesel production [ 17 , 18 ]. As a result, an emphasis has been placed on the underlying mechanisms, as well as the evaluation of the intermediates and final products produced.…”

Section: Introductionmentioning

confidence: 99%

Could termites be hiding a goldmine of obscure yet promising yeasts for energy crisis solutions based on aromatic wastes? A critical state-of-the-art review

Ali

Al-Tohamy

Mohamed

et al. 2022

Biotechnol Biofuels

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Biodiesel is a renewable fuel that can be produced from a range of organic and renewable feedstock including fresh or vegetable oils, animal fats, and oilseed plants. In recent years, the lignin-based aromatic wastes, such as various aromatic waste polymers from agriculture, or organic dye wastewater from textile industry, have attracted much attention in academia, which can be uniquely selected as a potential renewable feedstock for biodiesel product converted by yeast cell factory technology. This current investigation indicated that the highest percentage of lipid accumulation can be achieved as high as 47.25% by an oleaginous yeast strain, Meyerozyma caribbica SSA1654, isolated from a wood-feeding termite gut system, where its synthetic oil conversion ability can reach up to 0.08 (g/l/h) and the fatty acid composition in yeast cells represents over 95% of total fatty acids that are similar to that of vegetable oils. Clearly, the use of oleaginous yeasts, isolated from wood-feeding termites, for synthesizing lipids from aromatics is a clean, efficient, and competitive path to achieve "a sustainable development" towards biodiesel production. However, the lacking of potent oleaginous yeasts to transform lipids from various aromatics, and an unknown metabolic regulation mechanism presented in the natural oleaginous yeast cells are the fundamental challenge we have to face for a potential cell factory development. Under this scope, this review has proposed a novel concept and approach strategy in utilization of oleaginous yeasts as the cell factory to convert aromatic wastes to lipids as the substrate for biodiesel transformation. Therefore, screening robust oleaginous yeast strain(s) from wood-feeding termite gut system with a set of the desirable specific tolerance characteristics is essential. In addition, to reconstruct a desirable metabolic pathway/network to maximize the lipid transformation and accumulation rate from the aromatic wastes with the applications of various “omics” technologies or a synthetic biology approach, where the work agenda will also include to analyze the genome characteristics, to develop a new base mutation gene editing technology, as well as to clarify the influence of the insertion position of aromatic compounds and other biosynthetic pathways in the industrial chassis genome on the expressional level and genome stability. With these unique designs running with a set of the advanced biotech approaches, a novel metabolic pathway using robust oleaginous yeast developed as a cell factory concept can be potentially constructed, integrated and optimized, suggesting that the hypothesis we proposed in utilizing aromatic wastes as a feedstock towards biodiesel product is technically promising and potentially applicable in the near future.

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Section: Introductionmentioning

confidence: 99%

Could termites be hiding a goldmine of obscure yet promising yeasts for energy crisis solutions based on aromatic wastes? A critical state-of-the-art review

Ali

Al-Tohamy

Mohamed

et al. 2022

Biotechnol Biofuels

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“…Yeasts stand out as the most promising microorganisms in terms of microbial oils production since they can accumulate up to 80% of lipids per dry weight in their cells [ 1 ]. Moreover, they present high growth rates, short life cycles and versatility when it comes to utilization of different substrates [ 2 ]. High lipid contents have been achieved in yeasts using sugars as carbon sources [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Key role of fluorescence quantum yield in Nile Red staining method for determining intracellular lipids in yeast strains

Morales-Palomo

Liras

González‐Fernández

et al. 2022

Biotechnol Biofuels

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Background Microbial lipids are found to be an interesting green alternative to expand available oil sources for the chemical industry. Yeasts are considered a promising platform for sustainable lipid production. Remarkably, some oleaginous yeasts have even shown the ability to grow and accumulate lipids using unusual carbon sources derived from organic wastes, such as volatile fatty acids. Recent research efforts have been focused on developing rapid and accurate fluorometric methods for the quantification of intracellular yeast lipids. Nevertheless, the current methods are often tedious and/or exhibit low reproducibility. Results This work evaluated the reliability of different fluorescence measurements (fluorescence intensity, total area and fluorescence quantum yield) using Nile Red as lipid dye in two yeast strains (Yarrowia lipolytica ACA-DC 50109 and Cutaneotrichosporon curvatum NRRL-Y-1511). Different standard curves were obtained for each yeast specie. Fermentation tests were carried with 6-month difference to evaluate the effect of the fluorometer lamp lifetime on lipid quantification. Conclusions Fluorescence quantum yield presented the most consistent measurements along time and the closer estimations when compared with lipids obtained by conventional methods (extraction and gravimetrical determination). The need of using fluorescence quantum yield to estimate intracellular lipids, which is not the common trend in studies focused on microbial lipid production, was stressed. The information here provided will surely enable more accurate results comparison.

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“…Therefore, microalgae-based wastewater management has the potential to accomplish dual functions, including biofuel production and wastewater bioremediation. This strategy has the potential to significantly reduce costs associated with the production of microalgal lipids for biodiesel. , In contrast to terrestrial plants, microalgae are 10–50 times more efficient at CO 2 fixation . The consumption of CO 2 by photosynthesis in microalgal cultures reduces greenhouse gas emissions to the surroundings.…”

Section: Introductionmentioning

confidence: 99%

“…This strategy has the potential to significantly reduce costs associated with the production of microalgal lipids for biodiesel. 9,12 In contrast to terrestrial plants, microalgae are 10−50 times more efficient at CO 2 fixation. 18 The consumption of CO 2 by photosynthesis in microalgal cultures reduces greenhouse gas emissions to the surroundings.…”

Section: ■ Introductionmentioning

confidence: 99%

Simultaneous Promotion of Microalgal CO₂ Assimilation, Biomass Accumulation, Lipid Production, and Wastewater Nutrient Removal by Adding 5-Aminolevulinic Acid

Qiu

Cui

et al. 2022

ACS Sustainable Chem. Eng.

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Microalgae-based wastewater management has the potential to serve dual purposes, including biofuel production and wastewater bioremediation. The current study investigates the role of 5-aminolevulinic acid (5-ALA) in the regulation of photosynthesis, biomass accumulation, lipid production, and nutrient removal from wastewater by Chlorella pyrenoidosa. In wastewaters, a 3 μM concentration of 5-ALA was considered optimal, as it increased the photosynthetic light reaction and CO2 assimilation of C. pyrenoidosa cells, thereby facilitating cellular growth, metabolism, and biomass accumulation. The acceleration of algal cellular metabolism increased the substrate pool and enhanced the expression of lipogenic genes, thereby promoting lipid production. Meanwhile, the removal of nutrients from wastewater was accelerated after the addition of 5-ALA. The accelerated nutrient removal from wastewaters following 5-ALA treatment was related to C. pyrenoidosa cell growth. C. pyrenoidosa is capable of utilizing nitrogen and phosphorus from wastewater and accumulating biomass. The biomass from microalgae can be used as a feedstock to produce biofuels. Therefore, the current study provided a novel strategy for simultaneously promoting CO2 assimilation, biomass accumulation, lipid production, and wastewater nutrient removal with microalgae. It is possible to use microalgae for the management of wastewater if research and development on commercializing technologies based on microalgae, both upstream and downstream, are carried out continuously.

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Microbial lipids from organic wastes: Outlook and challenges

Cited by 58 publications

References 140 publications

Could termites be hiding a goldmine of obscure yet promising yeasts for energy crisis solutions based on aromatic wastes? A critical state-of-the-art review

Could termites be hiding a goldmine of obscure yet promising yeasts for energy crisis solutions based on aromatic wastes? A critical state-of-the-art review

Key role of fluorescence quantum yield in Nile Red staining method for determining intracellular lipids in yeast strains

Simultaneous Promotion of Microalgal CO₂ Assimilation, Biomass Accumulation, Lipid Production, and Wastewater Nutrient Removal by Adding 5-Aminolevulinic Acid

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Microbial lipids from organic wastes: Outlook and challenges

Cited by 58 publications

References 140 publications

Could termites be hiding a goldmine of obscure yet promising yeasts for energy crisis solutions based on aromatic wastes? A critical state-of-the-art review

Could termites be hiding a goldmine of obscure yet promising yeasts for energy crisis solutions based on aromatic wastes? A critical state-of-the-art review

Key role of fluorescence quantum yield in Nile Red staining method for determining intracellular lipids in yeast strains

Simultaneous Promotion of Microalgal CO2 Assimilation, Biomass Accumulation, Lipid Production, and Wastewater Nutrient Removal by Adding 5-Aminolevulinic Acid

Contact Info

Product

Resources

About

Simultaneous Promotion of Microalgal CO₂ Assimilation, Biomass Accumulation, Lipid Production, and Wastewater Nutrient Removal by Adding 5-Aminolevulinic Acid