Microbial lipopeptide production and purification bioprocesses, current progress and future challenges

Abstract

Lipopeoptides are amphiphilic compounds combining interesting physicochemical properties and biological activities. Due to their high foaming capacity in aerated bioreactor, the development of scalable bioprocesses for their production is a major bottleneck. In addition, the genes involved in the biosynthesis of these lipopeptides are mainly regulated by the quorum sensing, a global regulatory mechanism depending on cell density and known to be activated in biofilms. Several approaches have thus been considered in literature taking into account two criteria, on one side, to favor, control or avoid foam formation and on the other side, to use planktonic or immobilized (biofilm) cells. These different bioprocesses are discussed in the present review along with the purification strategies proposed for extracting and concentrating these biosurfactants.

Probing and Engineering the Fatty Acyl Substrate Selectivity of Starter Condensation Domains of Nonribosomal Peptide Synthetases in Lipopeptide Biosynthesis

“…Lipopeptides are structurally characterized as macrocyclic peptides with fatty acid modifications on the first amino acid of their peptide core. Cell membranes are generally the targets of bioactive lipopeptides; variations in the lengths and structures of the fatty acyl chains of natural lipopeptides are known to have significant effects on their respective biological activities . Notably, most lipopeptides isolated from their natural producers are actually a complex mixtures of compounds that share a cyclic peptide core but differ in the lengths and structures of their fatty acyl chains …”

Lipopéptidos producidos por agentes de control biológico del género Bacillus: revisión de herramientas analíticas utilizadas para su estudio

“…La extracción y purificación son etapas clave en el estudio y comercialización de lipopéptidos (Coutte et al, 2017). Generalmente, la purificación de estos compuestos inicia a partir de una matriz líquida (ej.…”

“…A pesar de que el uso de este método permite recuperar ~ 97% de los lipopéptidos presentes en el medio de cultivo, su principal desventaja es que no es un método selectivo, ya que otros compuestos (ej. péptidos) pueden coprecipitar con los lipopéptidos, obteniendo purezas por debajo de 60% (Coutte et al, 2017).…”

“…Recientemente, Coutte et al (2017) encontaron 263 lipopéptidos diferentes sintetizados por 11 géneros microbianos. Dentro de éstos, Bacillus representa el productor más abundante con 98 diferentes lipopéptidos (clasificados en familias), donde en el contexto del biocontrol de enfermedades de plantas, las tres familias de lipopéptidos de Bacillus: surfactinas, iturinas y fengycinas son de interés por su actividad antagonista para una amplia gama de fitopatógenos potenciales, incluidas bacterias, hongos y oomicetos (Ongena y Jacques, 2008).…”

Antioxidant properties of Enterobacter cloacae C3 lipopeptides in vitro and in model food emulsion

“…Microbial lipopeptides are amphiphilic secondary metabolites composed of one fatty acid chain linked to a peptide moiety (Jacques, 2011). These bioactive compounds are produced by a set of microorganisms mainly present in the soil (Coutte et al, 2017). Actually, lipopeptides are predominantly used in bioremediation of pollutants (Karlapudi et al, 2018), pharmaceutical (Gharaei-Fathabad, 2011), and cosmetic industries (Vecino, Cruz, Moldes, & Rodrigues, 2017); however, these microbial compounds exhibit a variety of useful properties for application in many sectors of food industry specially as antioxidant, antimicrobial, antiadhesive agents (Jemil et al, 2017), foaming, wetting, solubilizers, and emulsifiers (Banat, Makkar, & Cameotra, 2000).…”