Microbial Populations in Ruminal Liquid Samples from Young Beefmaster Bulls at Both Extremes of RFI Values

Manzanares-Miranda, Nelson; García-Mazcorro, José F.; Pérez-Medina, Eduardo B.; Vaquera-Vázquez, Anakaren; Martínez-Ruiz, Alejandro; Ramos-Zayas, Yareellys; Kawas, Jorge R.

doi:10.3390/microorganisms11030663

Cited by 7 publications

(2 citation statements)

References 58 publications

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“…Ruminococcaceae were thought as the manufacturer of SCFAs (Xie et al 2022 ) and were involved in fat degradation and fatty acid β-oxidation (Baars et al 2018 ; Zhang et al 2023 ). Succiniclasticum was a propionate producer and showed lower abundance in high efficiency animals (Manzanares-Miranda et al 2023 ).…”

Section: Discussionmentioning

confidence: 99%

Spatial profiles of the bacterial microbiota throughout the gastrointestinal tract of dairy goats

Hu,

Luo,

Cheng

et al. 2024

Appl Microbiol Biotechnol

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The gastrointestinal tract (GIT) is stationed by a dynamic and complex microbial community with functions in digestion, metabolism, immunomodulation, and reproduction. However, there is relatively little research on the composition and function of microorganisms in different GIT segments in dairy goats. Herein, 80 chyme samples were taken from ten GIT sites of eight Xinong Saanen dairy goats and then analyzed and identified the microbial composition via 16S rRNA V1-V9 amplicon sequencing. A total of 6669 different operational taxonomic units (OTUs) were clustered, and 187 OTUs were shared by ten GIT segments. We observed 264 species belonging to 23 different phyla scattered across ten GITs, with Firmicutes (52.42%) and Bacteroidetes (22.88%) predominating. The results revealed obvious location differences in the composition, diversity, and function of the GIT microbiota. In LEfSe analysis, unidentified_Lachnospiraceae and unidentified_Succinniclassicum were significantly enriched in the four chambers of stomach, with functions in carbohydrate fermentation to compose short-chain fatty acids. Aeriscardovia, Candidatus_Saccharimonas, and Romboutsia were significantly higher in the foregut, playing an important role in synthesizing enzymes, amino acids, and vitamins and immunomodulation. Akkermansia, Bacteroides, and Alistipes were significantly abundant in the hindgut to degrade polysaccharides and oligosaccharides, etc. From rumen to rectum, α-diversity decreased first and then increased, while β-diversity showed the opposite trend. Metabolism was the major function of the GIT microbiome predicted by PICRUSt2, but with variation in target substrates along the regions. In summary, GIT segments play a decisive role in the composition and functions of microorganisms. Key points • The jejunum and ileum were harsh for microorganisms to colonize due to the presence of bile acids, enzymes, faster chyme circulation, etc., exhibiting the lowest α-diversity and the highest β-diversity. • Variability in microbial profiles between the three foregut segments was greater than four chambers of stomach and hindgut, with a higher abundance of Firmicutes dominating than others. • Dairy goats dominated a higher abundance of Kiritimatiellaeota than cows, which was reported to be associated with fatty acid synthesis.

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Section: Discussionmentioning

confidence: 99%

Spatial profiles of the bacterial microbiota throughout the gastrointestinal tract of dairy goats

Hu,

Luo,

Cheng

et al. 2024

Appl Microbiol Biotechnol

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“…Prediction of functional potential, with Table S2: Summary of the 20 most significant features that were significantly different between Mexican and British patients, and Table S3: Summary of the 20 most significant features that were significantly different when using the variable disease*country with six levels (Mexico and U.K. for all three IBS subtypes). References [22,30,67,68] are cited in the supplementary materials.…”

Section: Supplementary Materialsmentioning

confidence: 99%

Fecal Microbial Composition and Predicted Functional Profile in Irritable Bowel Syndrome Differ between Subtypes and Geographical Locations

Garcia-Mazcorro,

Amieva-Balmori,

Triana-Romero

et al. 2023

Microorganisms

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Increasing evidence suggests a microbial pathogenesis in irritable bowel syndrome (IBS) but the relationship remains elusive. Fecal DNA samples from 120 patients with IBS, 82 Mexican (IBS-C: n = 33, IBS-D: n = 24, IBS-M: n = 25) and 38 British (IBS-C: n = 6, IBS-D: n = 27, IBS-M: n = 5), were available for analysis using 16S rRNA gene sequencing. Firmicutes (mean: 82.1%), Actinobacteria (10.2%), and Bacteroidetes (4.4%) were the most abundant taxa. The analysis of all samples (n = 120), and females (n = 94) only, showed no significant differences in bacterial microbiota, but the analysis of Mexican patients (n = 82) showed several differences in key taxa (e.g., Faecalibacterium) among the different IBS subtypes. In IBS-D there were significantly higher Bacteroidetes in British patients (n = 27) than in Mexican patients (n = 24), suggesting unique fecal microbiota signatures within the same IBS subtype. These differences in IBS-D were also observed at lower phylogenetic levels (e.g., higher Enterobacteriaceae and Streptococcus in Mexican patients) and were accompanied by differences in several alpha diversity metrics. Beta diversity was not different among IBS subtypes when using all samples, but the analysis of IBS-D patients revealed consistent differences between Mexican and British patients. This study suggests that fecal microbiota is different between IBS subtypes and also within each subtype depending on geographical location.

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Evaluation of Qazaq Aqbas bulls’ feed efficiency traits for breeding goals: A case study

Tilepova,

Arney,

Bostanova

et al. 2024

Smart Agricultural Technology

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Microbial Populations in Ruminal Liquid Samples from Young Beefmaster Bulls at Both Extremes of RFI Values

Cited by 7 publications

References 58 publications

Spatial profiles of the bacterial microbiota throughout the gastrointestinal tract of dairy goats

Spatial profiles of the bacterial microbiota throughout the gastrointestinal tract of dairy goats

Fecal Microbial Composition and Predicted Functional Profile in Irritable Bowel Syndrome Differ between Subtypes and Geographical Locations

Evaluation of Qazaq Aqbas bulls’ feed efficiency traits for breeding goals: A case study

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