Microbial protein production from CO2, H2, and recycled nitrogen: Focusing on ammonia toxicity and nitrogen sources

Yang, Xiaoyang; Xu, Mingyi; Zou, Rusen; Angelidaki, Irini; Zhang, Yifeng

doi:10.1016/j.jclepro.2021.125921

Cited by 41 publications

(13 citation statements)

References 42 publications

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“…Hydrogen-oxidizing Bacteria (HOB) have been under the spotlight in recent years due to their high growth rate, low resource requirements, and a high protein content (> 70% of their cell dry weight). Hence, they have great potential to be used as microbial protein (MP) source ( Christiaens et al, 2017 ; Yang et al, 2021 ). In recent studies, the growth of HOB on recycled nitrogen has been investigated ( Christiaens et al, 2017 ; Yang et al, 2021 ).…”

Section: Future Perspectives For Nitrogen Recoverymentioning

confidence: 99%

“…Hence, they have great potential to be used as microbial protein (MP) source ( Christiaens et al, 2017 ; Yang et al, 2021 ). In recent studies, the growth of HOB on recycled nitrogen has been investigated ( Christiaens et al, 2017 ; Yang et al, 2021 ). HOBs typically rely on ammonium as their nitrogen source and use H 2 and O 2 as electron donor and an electron acceptor, respectively, to fixate CO 2 as carbon source ( Yang et al, 2021 ).…”

Section: Future Perspectives For Nitrogen Recoverymentioning

confidence: 99%

“…In recent studies, the growth of HOB on recycled nitrogen has been investigated ( Christiaens et al, 2017 ; Yang et al, 2021 ). HOBs typically rely on ammonium as their nitrogen source and use H 2 and O 2 as electron donor and an electron acceptor, respectively, to fixate CO 2 as carbon source ( Yang et al, 2021 ). However, conventional ammonia removal and production with the Haber-Bosch process (an artificial industrial process to fixate nitrogen by converting atmospheric N 2 to NH 3 with hydrogen under high pressure and temperature) has a high energy cost.…”

Section: Future Perspectives For Nitrogen Recoverymentioning

confidence: 99%

“…A study on HOB species C. necator 335 confirmed its ability to grow at high NH 4 + loads. Hence, it is possible to grow MP from HOB via NH 4 + recovered from high NH 4 + load waste streams such as urine ( Yang et al, 2021 ). By supplementing human food or feed for cattle with MP from HOB produced from recovered nitrogen, nitrogen losses, energy costs, and surface area per unit of protein mass produced can be significantly reduced.…”

Section: Future Perspectives For Nitrogen Recoverymentioning

confidence: 99%

“…By supplementing human food or feed for cattle with MP from HOB produced from recovered nitrogen, nitrogen losses, energy costs, and surface area per unit of protein mass produced can be significantly reduced. This offers a strategy to tackle to challenges of future food production for an ever-growing human population ( Christiaens et al, 2017 ; Yang et al, 2021 ).…”

Section: Future Perspectives For Nitrogen Recoverymentioning

confidence: 99%

See 4 more Smart Citations

Development of Nitrogen Recycling Strategies for Bioregenerative Life Support Systems in Space

et al. 2021

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To enable long-distance space travel, the development of a highly efficient and robust system to recover nutrients from waste streams is imperative. The inability of the current physicochemical-based environmental control and life support system (ECLSS) on the ISS to produce food in situ and to recover water and oxygen at high enough efficiencies results in the need for frequent resupply missions from Earth. Therefore, alternative strategies like biologically-based technologies called bioregenerative life support systems (BLSSs) are in development. These systems aim to combine biological and physicochemical processes, which enable in situ water, oxygen, and food production (through the highly efficient recovery of minerals from waste streams). Hence, minimalizing the need for external consumables. One of the BLSS initiatives is the European Space Agency’s (ESA) Micro-Ecological Life Support System Alternative (MELiSSA). It has been designed as a five-compartment bioengineered system able to produce fresh food and oxygen and to recycle water. As such, it could sustain the needs of a human crew for long-term space exploration missions. A prerequisite for the self-sufficient nature of MELiSSA is the highly efficient recovery of valuable minerals from waste streams. The produced nutrients can be used as a fertilizer for food production. In this review, we discuss the need to shift from the ECLSS to a BLSS, provide a summary of past and current BLSS programs and their unique approaches to nitrogen recovery and processing of urine waste streams. In addition, compartment III of the MELiSSA loop, which is responsible for nitrogen recovery, is reviewed in-depth. Finally, past, current, and future related ground and space demonstration and the space-related challenges for this technology are considered.

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Section: Future Perspectives For Nitrogen Recoverymentioning

confidence: 99%

Section: Future Perspectives For Nitrogen Recoverymentioning

confidence: 99%

Section: Future Perspectives For Nitrogen Recoverymentioning

confidence: 99%

Section: Future Perspectives For Nitrogen Recoverymentioning

confidence: 99%

Section: Future Perspectives For Nitrogen Recoverymentioning

confidence: 99%

See 3 more Smart Citations

Development of Nitrogen Recycling Strategies for Bioregenerative Life Support Systems in Space

et al. 2021

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Enhanced Microbial Protein Production from CO₂ and Air by a MoS₂ Catalyzed Bioelectrochemical System

Zhang,

Zeng,

Wang

et al. 2024

ChemPlusChem

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Carbon dioxide can be relatively easily reduced to organic compounds in a bioelectrochemical system (BES). However, due to insufficient reduction force from in‐situ hydrogen evolution, it is difficult for nitrogen reduction. In this study, MoS2 was firstly used as an electrocatalyst for the simultaneous reduction of CO2 and N2 to produce microbial protein (MP) in a BES. Cell dry weight (CDW) could reach 0.81±0.04 g/L after 14 d operation at −0.7 V (vs. RHE), which was 108±3% higher than that from non−catalyst control group (0.39±0.01 g/L). The produced protein had a better amino acid profile in the BES than that in a direct hydrogen system (DHS), particularly for proline (Pro). Besides, MoS2 promoted the growth of bacterial cell on an electrode and improved the biofilm extracellular electron transfer (EET) by microscopic observation and electrochemical characterization of MoS2 biocathode. The composition of the microbial community and the relative abundance of functional enzymes revealed that MoS2 as an electrocatalyst was beneficial for enriching Xanthobacter and enhancing CO2 and N2 reduction by electrical energy. These results demonstrated that an efficient strategy to improve MP production of BES is to use MoS2 as an electrocatalyst to shift amino acid profile and microbial community.

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Comprehensive insights into sustainable conversion of agricultural and food waste into microbial protein for animal feed production

Rasool

Hussain

Shahzad

et al. 2023

Rev Environ Sci Biotechnol

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The growing global population and higher living standards instantly demand the transition in the direction of a sustainable food system. A substantial section of means and agricultural lands are presently committed to protein-rich feed production to rear livestock for human consumption. Conversely, accelerated farming activities and the food industry have rendered a drastic increase in waste which impair the economic and environmental sustainability of the ecosystem. This situation emerges the need for developing an integrated technology for waste management and to improve sustainability footprints. Microbial protein (MP) production based on renewable electron and carbon sources has the potential as a substitute protein source. MP production for animal feed use is growing fast and is derived from bacteria, algae, and fungi including yeast. MP produced from all types of microbes is currently commercialized and in use. However, novel methods and processes are also under investigation to make MP production more economical and sustainable. Current research on MP has concentrated on the valorization of waste materials by using high protein content-containing microorganisms, which can then be used in animal feed. Using such kind of integrated approach, the agroindustry waste resources upcycling can contribute towards finding sustainable, cheaper, and environment-friendly protein sources. This review first describes the potential waste feedstock for MP production and summarizes the recent progress in the application of MP-producing microorganisms including fungus, yeast, bacteria, and phototrophic microbes. Bioprocesses, and production technology advances for MP production have been explored and discussed in detail. Finally, the MP application as animal feed, its challenges, and future perspectives in research have been evaluated.

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Microbial protein production from CO2, H2, and recycled nitrogen: Focusing on ammonia toxicity and nitrogen sources

Cited by 41 publications

References 42 publications

Development of Nitrogen Recycling Strategies for Bioregenerative Life Support Systems in Space

Development of Nitrogen Recycling Strategies for Bioregenerative Life Support Systems in Space

Enhanced Microbial Protein Production from CO₂ and Air by a MoS₂ Catalyzed Bioelectrochemical System

Comprehensive insights into sustainable conversion of agricultural and food waste into microbial protein for animal feed production

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Microbial protein production from CO2, H2, and recycled nitrogen: Focusing on ammonia toxicity and nitrogen sources

Cited by 41 publications

References 42 publications

Development of Nitrogen Recycling Strategies for Bioregenerative Life Support Systems in Space

Development of Nitrogen Recycling Strategies for Bioregenerative Life Support Systems in Space

Enhanced Microbial Protein Production from CO2 and Air by a MoS2 Catalyzed Bioelectrochemical System

Comprehensive insights into sustainable conversion of agricultural and food waste into microbial protein for animal feed production

Contact Info

Product

Resources

About

Enhanced Microbial Protein Production from CO₂ and Air by a MoS₂ Catalyzed Bioelectrochemical System