Microbial Protein Synthesis and in vitro Fermentability of Fermented Oil Palm Fronds by Phanerochaete chrysosporium in Combination with Tithonia (Tithonia diversifolia) and Elephant Grass (Pennisetum purpureum)

Jamarun, Novirman; Pazla, Roni; Zain, Mardiati; Arief,

doi:10.3923/pjn.2018.462.470

Cited by 30 publications

(35 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The high output of NH3 in treatment B indicated that the rumen microbial activity was running well. NH3 concentration means the fermentability of feed related to protein digestibility, rumen microbial activity, and microbial population (Pazla et al 2018b). In addition, the high production of NH3 in treatment B was due to the increased degradation of crude protein in the rumen.…”

Section: Rumen Fluid Characteristics Of Fermented Tithoniamentioning

confidence: 99%

Quality evaluation of tithonia (Tithonia diversifolia) with fermentation using Lactobacillus plantarum and Aspergillus ficuum at different incubation times

et al. 2021

Self Cite

View full text Add to dashboard Cite

Abstract. Pazla R, Jamarun N, Zain M, Yanti G, Chandra RH. 2021. Quality evaluation of tithonia (Tithonia diversifolia) with fermentation using Lactobacillus plantarum and Aspergillus ficuum at different incubation times. Biodiversitas 22: 3936-3942. This research aimed to evaluate the nutritional quality of tithonia (Tithonia diversifolia) fermented using Lactobacillus plantarum and Aspergillus ficuum with different incubation times on nutritional content, digestibility, phytase enzyme activity, and characteristics of rumen fluid. The research used an experimental method with a factorial completely randomized design to evaluate the nutritional content and phytase enzyme activity after fermentation (stage 1). A randomized block design was used to evaluate in vitro digestibility and rumen fluid characteristics (stage 2). For factorial completely randomized design, factor A is the type of microbe (L. plantarum and A. ficuum), then factor B is the incubation time (3,5,7 days). Parameters observed were the nutritional content of dry matter (DM), organic matter (OM), crude protein (CP), crude fiber (CF), and phytase enzyme activity. For the Randomized Block Design, the research treatments were A = A. ficuum + 5 days of incubation, B = A. ficuum + 7 days of incubation, C = L. plantarum + 3 days of incubation, D = L. plantarum + 5 days of incubation. Parameters measured were the digestibility of dry matter(DMD), organic matter (OMD), crude protein (CPD), crude fiber (CFD), rumen pH, VFA production, and NH3 rumen fluid. The results showed that there was an interaction between the type of microbe and incubation time. The treatment had a significantly different effect (P<0.05) on the content of OM, CP, CF and phytase enzyme activity, but no interaction with the content of DM treatment. In the digestibility, the results showed that the effect was not significantly different (P>0.05) on DMD, OMD, CPD but had a significant effect (P<0.05) on CFD. The treatments also had no significant effect (P>0.05) on VFA but were significantly different (P<0.05) on NH3. From this study, it can be concluded that fermented tithonia using A. ficuum with an incubation period of 7 days could improve the quality of tithonia. It can be seen from the content of CP (31.02%), CF (16.52%), phytase enzyme activity (37.36 U/ml), DMD (66.86%), OMD (67.36%), CFD (81.01%), CPD (70.37%), VFA production 135 mM and NH3 concentration 14.31 mg/100 ml, and pH value 6.72 which is suitable for rumen microbial growth.

show abstract

Section: Rumen Fluid Characteristics Of Fermented Tithoniamentioning

confidence: 99%

Quality evaluation of tithonia (Tithonia diversifolia) with fermentation using Lactobacillus plantarum and Aspergillus ficuum at different incubation times

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…The high crude protein content in treatment B caused the effectiveness of rumen microbes to utilize protein to form microbial protein to be optimal so that it would increase the rumen microbial population, especially proteolytic bacteria. Pazla et al (2018b) explained that an increase in the microbial population would increase feed digestibility. The increase in crude protein content was caused by an increase in the mass of mold cells.…”

Section: Research Stage 2 a In Vitro Digestibilitymentioning

confidence: 99%

Lignin Content, Ligninase Enzyme Activity and in vitro Digestability of Sugarcane Shoots using Pleurotus ostreatus and Aspergillus oryzae at Different Fermentation Times

Pazla¹,

Jamarun²,

Warly³

et al. 2021

American Journal of Animal and Veterinary Sciences

Self Cite

View full text Add to dashboard Cite

This study aims to obtain the best lignin content, ligninase enzyme activity and in vitro digestibility value of fermented sugarcane shoots using Pleurotus ostreatus and Aspergillus oryzae at different fermentation times. The research process is divided into 2 stages. Stage 1 treatment samples include A1B1 = Sugarcane shoots fermented with Pleurotus ostreatus for 14 days; A1B2 = Sugarcane shoots fermented with Pleurotus ostreatus for 21 days; A1B3 = Sugarcane shoots fermented with Pleurotus ostreatus for 28 days; A2B1 = Sugarcane shoots fermented with Aspergillus oryzae for 14 days; A2B2 = Sugarcane shoots fermented with Aspergillus oryzae for 21 days; A2B3 = Sugarcane shoots fermented with Aspergillus oryzae for 28 days. The design used is a completely randomized design with a factorial pattern. Stage 2 treatment samples cover A = Sugarcane shoots fermented with Pleorotus ostreatus for 21 days; B = Sugarcane shoots fermented with Pleorotus ostreatus for 28 days; C = Sugarcane shoots fermented with Aspergillus oryzae for 21 days; D = Fermented sugarcane shoots with Aspergillus oryzae for 28 days. The design used was a randomized block design. The results showed that there was no interaction between the type of mold and the fermentation time on the lignin content (P>0.05), but there was an interaction with the enzyme activity of Laccase, LiP and MnP (P<0.05). Also, there were significant differences in the digestibility of protein, cellulose, hemicellulose, VFA and NH3 (P<005), however the digestibility of DM, OM, ADF, NDF and rumen fluid pH had no significant difference (P>0.05). It was concluded that sugarcane shoots fermented with Pleurotus ostreatus mold for 28 days got the best results with the value as follow, lignin content (11.55%), CP digestibility (57.90%), cellulose digestibility (50.25%), Hemicellulose digestibility (62.65%), Laccase enzyme activity (2.68 U/mL), LiP enzyme activity (19.44 U/mL), VFA (111.67 mM) and NH3 (10.48 mg/100 mL).

show abstract

“…The resulting balance of NH3 and VFA concentrations was also a factor causing the treatment's pH value not significantly different. Pazla et al (2018a) stated that the rumen's pH value is a balanced interaction between the buffer capacity with the alkalinity and acidity of fermentation products.…”

Section: Rumen Fluid Characteristicsmentioning

confidence: 99%

“…Genther and Hansen (2014) stated that rumen microbes need mineral supplementation to fulfill their normal function to stimulate proliferation and fermentation activity. Mineral P is a mineral needed for ATP synthesis and microbial protein synthesis (Karsli and Russell, 2001;Febrina et al, 2020;Pazla et al, 2018a).…”

Section: Nutrient Digestibilitymentioning

confidence: 99%

“…Sticks and fronds are parts of oil palm, which contain high lignin. lignin is a wood substance that cannot be broken down by rumen microbes, causing low digestibility (Jamarun et al, 2018) maximum inclusion is carried out in the form of processing through feed technology to optimize PLF as animal feed, one of which is ammoniation. Ammoniation can loosen the lignocellulose and lignohemicellulose bonds that exist in plants and increase the CP content sourced from Urea (Suryani et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Optimization of Rumen Bioprocess through The Addition of Phosphorus and Sulfur Minerals on Ammoniated Palm Leaves and Fronds (Elaeis Guineensis Jacq.)

Suyitman¹,

Warly²,

Hellyward³

et al. 2021

American Journal of Animal and Veterinary Sciences

Self Cite

View full text Add to dashboard Cite

Microbial Protein Synthesis and in vitro Fermentability of Fermented Oil Palm Fronds by Phanerochaete chrysosporium in Combination with Tithonia (Tithonia diversifolia) and Elephant Grass (Pennisetum purpureum)

Cited by 30 publications

References 21 publications

Quality evaluation of tithonia (Tithonia diversifolia) with fermentation using Lactobacillus plantarum and Aspergillus ficuum at different incubation times

Quality evaluation of tithonia (Tithonia diversifolia) with fermentation using Lactobacillus plantarum and Aspergillus ficuum at different incubation times

Lignin Content, Ligninase Enzyme Activity and in vitro Digestability of Sugarcane Shoots using Pleurotus ostreatus and Aspergillus oryzae at Different Fermentation Times

Optimization of Rumen Bioprocess through The Addition of Phosphorus and Sulfur Minerals on Ammoniated Palm Leaves and Fronds (Elaeis Guineensis Jacq.)

Contact Info

Product

Resources

About