Background: Heavy metal pollutants pose a significant environmental and public health threat, necessitating effective remediation strategies. Genetically modified microorganisms, Shewanella oneidensis MR-1 and Cupriavidus metallidurans CH34, show promise in mitigating contamination. Specific Background: Engineered bacteria have been designed to improve metal absorption, specifically lead, mercury, cadmium, and zinc, but further research is needed to understand their effectiveness in different pH levels and water conditions. Knowledge Gap: Few studies have investigated the pH-dependent efficiency of genetically engineered microbes in heavy metal absorption or their performance under wastewater conditions, despite numerous studies on microbial bioremediation. Aims: The study evaluates the efficacy of genetically modified Shewanella oneidensis and Cupriavidus metallidurans in removing heavy metals from contaminated water at various pH levels. Results: The study found that both bacterial strains effectively removed 91% of lead at pH 7, with optimal levels for lead and cadmium absorption depending on the specific pH. Novelty: CRISPR/Cas9 technology is used for genetic modification of strains, enhancing microbial engineering for environmental remediation. Study provides new data on pH-specific absorption rates.. Implications: These findings can be applied to enhance the design of bioreactors and biofiltration systems, offering a sustainable solution to heavy metal contamination in wastewater, with broad potential for industrial and environmental applications.
Highlights:
Modified bacteria remove 91% of lead at neutral pH.
CRISPR/Cas9 enhances bacteria for efficient heavy metal removal.
pH optimization is key for effective heavy metal absorption.
Keywords: Heavy metals, Bioremediation, Genetically modified bacteria, Shewanella oneidensis, Cupriavidus metallidurans
