Microbial screening for quinolones residues in cow milk by bio-optical method

Appicciafuoco, Brunella; Dragone, Roberto; Frazzoli, Chiara; Bolzoni, G.; Mantovani, Alberto; Ferrini, Anna Maria

doi:10.1016/j.jpba.2014.11.037

Cited by 21 publications

(9 citation statements)

References 18 publications

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“…Toxicity was also assessed in liquid culture in which overnight cultures were diluted to an OD 600 of 0.1 in fresh LB containing 50 µg mL −1 ampicillin in the absence or presence of 0.2% (wt/vol) arabinose, and their absorbance at OD 600 was monitored using the Bioscreen C System (Oy Growth Curves Ab Ltd., Helsinki, Finland) while growing at 37°C. The resulting data were used to construct growth curves in accordance with a method described previously ( 42 ).…”

Section: Methodsmentioning

confidence: 99%

A novel double-ribonuclease toxin-antitoxin system linked to the stress response and survival of Acidovorax citrulli

Wang,

Kan,

Bai

et al. 2023

Microbiol Spectr

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Toxin-antitoxin (TA) systems are widespread among prokaryotes and can play an important role in the maintenance of genetic elements and pathogen virulence. Their roles in bacterial stress response and antimicrobial persistence currently remain highly controversial. The current study investigated a novel TA system (Aave_1720-Aave_1719) from the phytopathogenic bacterium Acidovorax citrulli , which had characteristics of both type II and type V TA systems. Heterologous expression in Escherichia coli confirmed that Aave_1720 functions as a toxin gene, while Aave_1719 acts as antitoxin gene. The Aave_1720 toxin functions as a ribonuclease, and antitoxin Aave_1719 binds directly to Aave_1720 to inhibit its ribonuclease activity, which is typical of type II TA systems. Interestingly, Aave_1719 itself was found to exhibit ribonuclease activity with a preference for targeting Aave_1720 mRNA, which indicates that it can also neutralize the Aave_1720 toxin in a manner characteristic of type V TA systems. Expression analysis revealed that the Aave_1719 gene is upregulated in response to sodium hypochlorite stress and that deletion of the Aave_1720 gene individually or in combination with Aave_1719 affected biofilm formation, as well as the survival of A. citrulli in response to sodium hypochlorite stress. Taken together, these findings expand our understanding of TA systems revealing a novel mechanism of toxin neutralization that combines aspects of both type II and type V TA systems. IMPORTANCE Bacterial fruit blotch (BFB), which is caused by the seed-borne bacterium Acidovorax citrulli , is a devastating disease affecting cucurbit crops throughout the world. Although seed fermentation and treatment with disinfectants can provide effective management of BFB, they cannot completely guarantee pathogen-free seedstock, which suggests that A. citrulli is a highly stress-resistant pathogen. Toxin-antitoxin (TA) systems are common among a diverse range of bacteria and have been reported to play a role in bacterial stress response. However, there is currently much debate about the relationship between TA systems and stress response in bacteria. The current study characterized a novel TA system (Aave_1720-Aave_1719) from A. citrulli that affects both biofilm formation and survival in response to sodium hypochlorite stress. The mechanism of neutralization differed from typical TA systems as two separate mechanisms were associated with the antitoxin, which exhibited characteristics of both type II and type V TA systems. The Aave_1720-Aave_1719 system described here also constitutes the first known report of a double-ribonuclease TA system in bacteria, which expands our understanding of the range of regulatory mechanisms utilized by bacterial TA systems, providing new insight into the survival of A. citrulli in response to stress.

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Section: Methodsmentioning

confidence: 99%

A novel double-ribonuclease toxin-antitoxin system linked to the stress response and survival of Acidovorax citrulli

Wang,

Kan,

Bai

et al. 2023

Microbiol Spectr

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“…The most appropriate cellular concentration of yeast stock suspension used in the present work (1.5 × 10 8 colony forming unit/mL or cfu/mL) and the 1:100 dilution ratio for the bioassays were defined by preliminary tests at different S. cerevisiae cells/simazine dose ratios. Indeed, sensitivity and limit of detection (LoD) of a cytotoxicity assay mainly depend from the number of cells/chemical contaminant dose ratio [36]. Turbidimetric measurements of optical density at the wavelength of 525 nm (OD 525nm ) were performed on eight serial dilutions of a yeast cell stock suspension (yeast stock suspension 1.5 × 10 8 cfu/mL) in quartz cuvettes with 1 cm optical path lengths against water as reference (UV2 UV/Visible, Unicam Instruments, Cambridge, UK).…”

Section: Methodsmentioning

confidence: 99%

Towards Simazine Monitoring in Agro-Zootechnical Productions: A Yeast Cell Bioprobe for Real Samples Screening

et al. 2018

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Simazine is an herbicide that is able to contaminate surface waters, ground waters, and milk/dairy products, thus posing concerns in both environmental health and food safety. A yeast-based bioprobe was utilized to detect simazine in spiked real samples of livestock drinking water and raw cow’s milk. Yeast aerobic respiration was taken as short-term toxicological endpoint. We carried out comparative measures of yeast oxygen consumption between simazine-spiked samples and blank samples. Percentage interference (%ρ) on yeast aerobic respiration was calculated through the comparison of aerobic respiration of simazine-exposed and non-exposed yeast cells. The method was optimized for raw cow’s milk samples by using boric acid as fungistatic agent in order to avoid cellular proliferation. Overall, the results have shown that simazine can be detected up to concentrations five times below the EU legal concentration limits for drinking water (0.02 ppb) and cow’s milk (2 ppb) (%ρ values of 18.53% and 20.43% respectively; %RSD ≤ 15%). Dose-effect relationships of simazine were assessed. The findings of the bioassays match reasonably well with known mechanisms of toxicity and intracellular detoxification in yeast. A correlation between fat content in milk samples and analytical performance of the bioprobe was established. Results suggest the involvement of a matrix effect, presumably due to lipid sequestration of simazine. The yeast-based bioprobe has proved to be sensitive and suitable for the detection of simazine in real samples in concentrations of interest.

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“…Against this background, such critical issues have produced a great demand for simplified, sensitive, and rapid screening methods (2, 3), without (or with reduced) sample pretreatments, suitable for environmental monitoring and surveillance at critical control points throughout the entire agro-food supply chain. In fact, recent progress and challenges in the field of analytical chemistry are focused on improving analytical methods with reduced environmental impact and developing new analytical sensoristic devices for continuous monitoring and diagnostics oriented toward environmental health and food safety.…”

Section: Environmental Health and Food Safety: Scenario And Needsmentioning

confidence: 99%

Portable Bio/Chemosensoristic Devices: Innovative Systems for Environmental Health and Food Safety Diagnostics

Dragone

Grasso

Muccini

et al. 2017

Front. Public Health

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This mini-review covers the newly developed biosensoristic and chemosensoristic devices described in recent literature for detection of contaminants in both environmental and food real matrices. Current needs in environmental and food surveillance of contaminants require new simplified, sensitive systems, which are portable and allow for rapid and on-site monitoring and diagnostics. Here, we focus on optical and electrochemical bio/chemosensoristic devices as promising tools with interesting analytical features that can be potentially exploited for innovative on-site and real-time applications for diagnostics and monitoring of environmental and food matrices (e.g., agricultural waters and milk). In near future, suitably developed and implemented bio/chemosensoristic devices will be a new and modern technological solution for the identification of new quality and safety marker indexes as well as for a more proper and complete characterization of abovementioned environmental and food matrices. Integrated bio/chemosensoristic devices can also allow an “holistic approach” that may prove to be more suitable for diagnostics of environmental and food real matrices, where the copresence of more bioactive substances is frequent. Therefore, this approach can be focused on the determination of net effect (mixture effect) of bioactive substances present in real matrices.

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Microbial screening for quinolones residues in cow milk by bio-optical method

Cited by 21 publications

References 18 publications

A novel double-ribonuclease toxin-antitoxin system linked to the stress response and survival of Acidovorax citrulli

A novel double-ribonuclease toxin-antitoxin system linked to the stress response and survival of Acidovorax citrulli

Towards Simazine Monitoring in Agro-Zootechnical Productions: A Yeast Cell Bioprobe for Real Samples Screening

Portable Bio/Chemosensoristic Devices: Innovative Systems for Environmental Health and Food Safety Diagnostics

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