Microbial trehalose boosts the ecological fitness of biocontrol agents, the viability of probiotics during long-term storage and plants tolerance to environmental-driven abiotic stress

Onwe, Reuben O.; Onwosi, Chukwudi O.; Ezugworie, Flora N.; Ekwealor, C. C.; Okonkwo, Chigozie C.

doi:10.1016/j.scitotenv.2021.150432

Cited by 27 publications

(13 citation statements)

References 149 publications

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“…It is evident that there is an increasing demand for sustainable agronomy to meet the needs of the growing population and enhance agronomic yield without disturbing ecological components. Since 2000, there has been an increase in the demand for bio stimulants as growth promoters, and research interest and publication are increasing in the area of heat stress management using beneficial microbes [ 126 , 127 ]. Microorganism inoculation enhances resistance and tolerance towards heat stress.…”

Section: Conclusion and Future Prospectivementioning

confidence: 99%

A Review on the Role of Endophytes and Plant Growth Promoting Rhizobacteria in Mitigating Heat Stress in Plants

et al. 2022

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Among abiotic stresses, heat stress is described as one of the major limiting factors of crop growth worldwide, as high temperatures elicit a series of physiological, molecular, and biochemical cascade events that ultimately result in reduced crop yield. There is growing interest among researchers in the use of beneficial microorganisms. Intricate and highly complex interactions between plants and microbes result in the alleviation of heat stress. Plant–microbe interactions are mediated by the production of phytohormones, siderophores, gene expression, osmolytes, and volatile compounds in plants. Their interaction improves antioxidant activity and accumulation of compatible osmolytes such as proline, glycine betaine, soluble sugar, and trehalose, and enriches the nutrient status of stressed plants. Therefore, this review aims to discuss the heat response of plants and to understand the mechanisms of microbe-mediated stress alleviation on a physio-molecular basis. This review indicates that microbes have a great potential to enhance the protection of plants from heat stress and enhance plant growth and yield. Owing to the metabolic diversity of microorganisms, they can be useful in mitigating heat stress in crop plants. In this regard, microorganisms do not present new threats to ecological systems. Overall, it is expected that continued research on microbe-mediated heat stress tolerance in plants will enable this technology to be used as an ecofriendly tool for sustainable agronomy.

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Section: Conclusion and Future Prospectivementioning

confidence: 99%

A Review on the Role of Endophytes and Plant Growth Promoting Rhizobacteria in Mitigating Heat Stress in Plants

et al. 2022

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“…The fact that otsB deletion resulted in an induction of the otsBA operon suggests that, in this mutant, the cells accumulate the phosphor-sugar trehalose-6P as the phosphatase OtsB is nonfunctional. Whether tolerance to salt and symbiosis efficiency improvements are due to higher levels of trehalose-6P in the ∆otsB strain remains to be determined but further supports the idea that trehalose and its phosphorylated derivative are key metabolites for stress adaptation and plant growth promotion notably under abiotic stress [46]. Interestingly, Sengupta and colleagues previously showed in yeast that trehalose phosphate synthase methylation by a Cystein methyl transferase resulted in increased trehalose-6 phosphate biosynthesis [47], further supporting possible interplay between SAM-generating methionine cycling and trehalose metabolism.…”

Section: Resultsmentioning

confidence: 59%

Analysis of Ensifer aridi Mutants Affecting Regulation of Methionine, Trehalose, and Inositol Metabolisms Suggests a Role in Stress Adaptation and Symbiosis Development

2022

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Isolated from desert, the nitrogen-fixing bacterium Ensifer aridi LMR001 is capable of survival under particularly harsh environmental conditions. To obtain insights in molecular mechanisms involved in stress adaptation, a recent study using RNAseq revealed that the RpoE2-mediated general stress response was activated under mild saline stress but appeared non-essential for the bacterium to thrive under stress and develop the symbiosis. Functions associated with the stress response included the metabolisms of trehalose, methionine, and inositol. To explore the roles of these metabolisms in stress adaptation and symbiosis development, and the possible regulatory mechanisms involved, mutants were generated notably in regulators and their transcriptions were studied in various mutant backgrounds. We found that mutations in regulatory genes nesR and sahR of the methionine cycle generating S-adenosylmethionine negatively impacted symbiosis, tolerance to salt, and motility in the presence of NaCl. When both regulators were mutated, an increased tolerance to detergent, oxidative, and acid stresses was found, suggesting a modification of the cell wall components which may explain these phenotypes and support a major role of the fine-tuning methylation for symbiosis and stress adaptation of the bacterium. In contrast, we also found that mutations in the predicted trehalose transport and utilization regulator ThuR and the trehalose phosphate phosphatase OtsB-encoding genes improved symbiosis and growth in liquid medium containing 0.4 M of NaCl of LMR001ΔotsB, suggesting that trehalose metabolism control and possibly trehalose-6 phosphate cellular status may be biotechnologically engineered for improved symbiosis under stress. Finally, transcriptional fusions of gfp to promoters of selected genes and expression studies in the various mutant backgrounds suggest complex regulatory interplay between inositol, methionine, and trehalose metabolic pathways.

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“…Different initial pH values (4,5,6,7,8,9) were adjusted to determine the optimal initial pH for trehalose production.…”

Section: Initial Phmentioning

confidence: 99%

“…The effects of different inoculum levels on the production of trehalose were investigated, and the results are shown in Fig. (4). The highest possible trehalose yield (17.24 &12.41mg ml -1…”

Section: Effect Of Level Of Inoculummentioning

confidence: 99%

“…Several recent studies have demonstrated the involvement of trehalose in numerous signaling and metabolic pathways in plants, as well as its vital role in plant growth and development and the achievement of stress (e.g. drought, salinity and temperature) tolerance [4]. Trehalose is a substance that is present in many different types of life, including plants, invertebrates, and microorganisms [5,6] demonstrated that trehalose can protect organisms from external challenges such as heat, alcohol, osmotic, and oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

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The Role of Trehalose-producing Bradyrhizobium japonicum and Azotobacter chroococcum in Enhancing Salinity Tolerance of Zea mays Plants

Ali

Orf

2022

JAMB

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Salinity is one of the most potent abiotic elements in nature and is damaging to both plants and microbes. Osmostress response in bacteria involves the accumulation of small organic compounds called compatible solutes as trehalose. In this work the synthesis and accumulation of trehalose by Bradyrhizobium japonicum strain (ARC 517) were investigated. Different sources of carbon, nitrogen, initial pH, and inoculum level were studied in order to increase trehalose productivity. An optimal production medium containing glucose and yeast extract was found suitable for trehalose production. The results showed that keeping the pH of the culture broth at 6.0 is important for trehalose production. Moreover the optimal level of inoculum was 4.0%. The optimized parameters gave a maximum trehalose production of 22.65 mg ml-1. Scanning electron microscope showed that cells of B. japonicum that enhanced for trehalose was aggregated and became the longest with length about 3 times higher than that of control. The results of field experiment revealed that, the Zea mays plants treated with B. japonicum strain (ARC 517) that enhanced for trehalose + Azotobacter chroococcum retained higher relative water content (RWC), chlorophyll content, K+/Na+ ratio as compared to other treatments. Positive correlation between trehalose overproduction and high-yield parameters were observed under saline conditions. These findings suggest that trehalose overproduction could be a beneficial characteristic for biofertilizers.

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Microbial trehalose boosts the ecological fitness of biocontrol agents, the viability of probiotics during long-term storage and plants tolerance to environmental-driven abiotic stress

Cited by 27 publications

References 149 publications

A Review on the Role of Endophytes and Plant Growth Promoting Rhizobacteria in Mitigating Heat Stress in Plants

A Review on the Role of Endophytes and Plant Growth Promoting Rhizobacteria in Mitigating Heat Stress in Plants

Analysis of Ensifer aridi Mutants Affecting Regulation of Methionine, Trehalose, and Inositol Metabolisms Suggests a Role in Stress Adaptation and Symbiosis Development

The Role of Trehalose-producing Bradyrhizobium japonicum and Azotobacter chroococcum in Enhancing Salinity Tolerance of Zea mays Plants

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