Microbiochemical Analysis of Changes in Proteoglycan and Collagen in Joint Tissues During the Development of Antigen-induced Arthritis in the Rabbit

Beesley, Julian E.; Jessup, Eileen; Pettipher, Roy; Henderson, Brian E.

doi:10.1016/s0934-8832(11)80061-2

Cited by 10 publications

(9 citation statements)

References 12 publications

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“…Furthermore, a loss in components of joint matrices that included a generalized decrease in glycosaminoglycans and localized decrease in collagen staining was noted in inflamed TMJs. These histopathologic features of antigeninduced arthritis of the juvenile TMJ are similar to those observed previously in experimental arthritis of the knee joint (Caulfield et al, 1982;Edwards et al, 1988;Beesley et al, 1992) and in human rheumatoid arthritis (reviewed in Harris, 1989). While this rabbit model of TMJ arthritis requires further cephalometric characterization and may potentially demonstrate some morphologic differences from that observed in humans with JRA of the TMJ, our previous find- ings (Kapila et al, 1995), together with those of the present study, suggest that this animal model may be useful for further delineating some of the processes that lead to aberrant mandibulofacial development in juvenile TMJ arthritis.…”

Section: Discussionsupporting

confidence: 85%

Mandibulofacial Adaptations in a Juvenile Animal Model of Temporomandibular Joint Arthritis

Tavakkoli-Jou

Kapila

1999

J Dent Res

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Juvenile rheumatoid arthritis (JRA) is a chronic systemic disease of childhood that affects synovial joints including the temporomandibular joint (TMJ). Individuals with JRA of the TMJ frequently show aberrations in mandibulofacial development. Since the basis for these developmental perturbations is poorly understood, they remain a perplexing clinical problem to manage. To begin dissecting the mechanisms for altered craniofacial development in JRA of the TMJ, we characterized the gross morphologic adaptations in the facial skeleton in a juvenile animal model of TMJ arthritis. Arthritis was induced in ten 87-day-old male rabbits by intra-articular challenge with ovalbumin. Eight sham-challenged and 4 unchallenged rabbits were used as controls. Serial lateral head cephalograms, taken at 73 (T1), 87 (T2), 108 (T3), 129 (T4), and 150 (T5) days of age, were evaluated by linear measures of maxillary, mandibular, and posterior dental height dimensions. Differences in the absolute dimensions and relative percent incremental changes were compared by ANOVA and Fisher's test. The body weights, as well as the absolute measures and incremental changes in maxillary and posterior dental height dimensions, were not significantly different between the antigen-challenged and control groups. In contrast, absolute measures of posterior mandibular height, condylar neck height, and total mandibular length were significantly smaller (P < 0.05) in antigen-challenged rabbits than in both control groups at T5. Furthermore, the antigen-challenged rabbits demonstrated significantly smaller (P < 0.05) relative increases in all measures of mandibular length, and in total posterior mandibular and condylar neck heights. Cephalometric superimpositions on the cranial base and tantalum implants confirmed these quantitative observations. This investigation demonstrates mandibulofacial developmental aberrations in experimental JRA-like disease of the TMJ that are similar to those observed in humans with this disease.

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Section: Discussionsupporting

confidence: 85%

Mandibulofacial Adaptations in a Juvenile Animal Model of Temporomandibular Joint Arthritis

Tavakkoli-Jou

Kapila

1999

J Dent Res

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“…Our observations in rabbit immune arthritis agree with other reports on the pattern of chondrocyte-mediated cartilage degradation [6] and synovial fibrosis [2,6]. The correlation between magnetic resonance properties and proteoglycan content in human cadaveric cartilage has been studied [-7, 8].…”

Section: Discussionsupporting

confidence: 90%

Comparison of magnetic resonance imaging (MRI) and histopathology in rabbit models of osteoarthritis and immune arthritis

et al. 1993

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Osteoarthritis was surgically induced in mature male Dutch Belted rabbits by sectioning the fibular collateral and sesamoid ligaments and removal of the anterior horn of the lateral meniscus. The site of surgical intervention was detectable by MRI. Histopathologic analysis revealed severe focal cartilage lesions on opposing surfaces of the tibia and femur. Histology of cartilage adjacent to the osteoarthritic lesions appeared normal. In another animal model, arthritis was induced by immunization against ovalbumin followed by intra-articular injection of ovalbumin. MRI of immune arthritic rabbit knees showed accumulation of synovial fluid and cartilage degradation. Histopathology was characterized by vascular necrosis of the synovium and depletion of cartilage proteoglycan. MRI can be used to non-invasively follow the therapeutic effects of drug treatment on synovial inflammation and cartilage degradation in rabbit knees.

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“…To semi‐quantify the collagen content in the cartilage, one way to visualize collagen fibers in histological sections is by staining with picrosirius red (Beesley et al. ; Schmitz et al. ).…”

Section: Methodsmentioning

confidence: 99%

Alteration of cartilage surface collagen fibers differs locally after immobilization of knee joints in rats

et al. 2015

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The purpose of this study was to examine the ultrastructural changes of surface cartilage collagen fibers, which differ by region and the length of the experimental period in an immobilization model of rat. Male Wistar rats were randomly divided into histological or macroscopic and ultrastructural assessment groups. The left knees of all the animals were surgically immobilized by external fixation for 1, 2, 4, 8 or 16 weeks (n = 5/time point). Sagittal histological sections of the medial mid-condylar region of the knee were obtained and assessed in four specific regions (contact and peripheral regions of the femur and tibia) and two zones (superficial and deep). To semi-quantify the staining intensity of the collagen fibers in the cartilage, picrosirius red staining was used. The cartilage surface changes of all the assessed regions were investigated by scanning electron microscopy (SEM). From histological and SEM observations, the fibrillation and irregular changes of the cartilage surface were more severe in the peripheral region than in the contact region. Interestingly, at 16 weeks post-immobilization, we observed non-fibrous structures at both the contact and peripheral regions. The collagen fiber staining intensity decreased in the contact region compared with the peripheral region. In conclusion, the alteration of surface collagen fiber ultrastructure and collagen staining intensity differed by the specific cartilage regions after immobilization. These results demonstrate that the progressive degeneration of cartilage is region specific, and depends on the length of the immobilization period.

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Microbiochemical Analysis of Changes in Proteoglycan and Collagen in Joint Tissues During the Development of Antigen-induced Arthritis in the Rabbit

Cited by 10 publications

References 12 publications

Mandibulofacial Adaptations in a Juvenile Animal Model of Temporomandibular Joint Arthritis

Mandibulofacial Adaptations in a Juvenile Animal Model of Temporomandibular Joint Arthritis

Comparison of magnetic resonance imaging (MRI) and histopathology in rabbit models of osteoarthritis and immune arthritis

Alteration of cartilage surface collagen fibers differs locally after immobilization of knee joints in rats

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