Microbiological changes, shelf life and identification of initial and spoilage microbiota of sea bream fillets stored under various conditions using <scp>16S rRNA</scp> gene analysis

Parlapani, Foteini F.; Kormas, Konstantinos Ar.; Boziaris, Ioannis S.

doi:10.1002/jsfa.6957

Cited by 70 publications

(55 citation statements)

References 62 publications

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“…fluorescens is not the most abundant species during sea bream spoilage in every case. Indeed, P. fragi was found to be the predominant microorganism in both whole sea bream and sea bream fillets stored at chill temperatures (Parlapani et al, 2013;Parlapani, Kormas, et al, 2014). However, P. fragi was not isolated from gutted sea bream in our study.…”

Section: Identification Of Pseudomonas Grown On Cfc Mediumcontrasting

confidence: 78%

“…3). Phylotype GSBb11 presented 99% similarity with the bacterium SBFeB5-d0 which was isolated from sea bream fillets (Parlapani, Kormas, et al, 2014) and the uncultured bacterium clone SB-d16-2 originated from iced whole sea bream (Parlapani et al, 2013). Its closest characterized relative was Pseudomonas fluorescens strain hswx163 (Wei S., unpublished, GenBank) (Fig.…”

Section: Identification Of Pseudomonas Grown On Cfc Mediummentioning

confidence: 94%

“…However, P. fragi was not isolated from gutted sea bream in our study. Phylogenetic relationship of phylotypes reported in this work in relation to phylotypes found in iced whole sea bream, designed as SB (Parlapani et al, 2013) and chilled sea bream fillets, designed as SBF (Parlapani, Kormas, et al, 2014) is clearly delineated in Fig. 3.…”

Section: Identification Of Pseudomonas Grown On Cfc Mediummentioning

confidence: 97%

“…A colony PCR method was used for the amplification of 16S rRNA gene fragments using bacteria cells directly as the template (Parlapani, Kormas, et al, 2014). Approximately 900 bp of the 16S rRNA gene were amplified by PCR with universal primers 27fBAC (5 0 -AGAGTTTGATCMTGGCTCAG-3 0 , M ¼ A/C) (Lane, 1991) and 907r BAC (5 0 -CCCGTCAATTCCTTTGAGTTT-3 0 ) (Muyzer, Teske, Wirsen, & Jannasch, 1995) on a thermal cycler (MyCycler, Bio-Rad Inc., USA).…”

Section: Pcr Amplification and Isolates Purificationmentioning

confidence: 99%

“…have been recognized as SSOs of sea bream stored aerobically at low temperatures (Koutsoumanis, Lampropoulou, & Nychas, 1999;Parlapani, Kormas, & Boziaris, 2014;Parlapani, Mallouchos, Haroutounian, & Boziaris, 2014;Parlapani, Meziti, Kormas, & Boziaris, 2013;Tryfinopoulou, Tsakalidou, & Nychas, 2002). However, no information is available regarding the effect of chill storage on Pseudomonas diversity in gutted sea bream based on 16S rRNA gene sequence analysis.…”

Section: Introductionmentioning

confidence: 99%

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The dynamics of Pseudomonas and volatilome during the spoilage of gutted sea bream stored at 2 °C

et al. 2015

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Section: Identification Of Pseudomonas Grown On Cfc Mediumcontrasting

confidence: 78%

Section: Identification Of Pseudomonas Grown On Cfc Mediummentioning

confidence: 94%

Section: Identification Of Pseudomonas Grown On Cfc Mediummentioning

confidence: 97%

Section: Pcr Amplification and Isolates Purificationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The dynamics of Pseudomonas and volatilome during the spoilage of gutted sea bream stored at 2 °C

et al. 2015

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Microbiological status based on 454-pyrosequencing and volatilome analysis of gilthead seabream (Sparus aurata) fed on diets with hydrolyzed feather meal and poultry by-product meal as fishmeal replacers

Parlapani

Boziaris

Meziti

et al. 2019

Eur Food Res Technol

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Host-associated and Environmental Microbiomes in an Open-Sea Mediterranean Gilthead Sea Bream Fish Farm

et al. 2022

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Gilthead seabream is among the most important farmed fish species in the Mediterranean Sea. Several approaches are currently applied to assure a lower impact of diseases and higher productivity, including the exploration of the fish microbiome and its manipulation as a sustainable alternative to improve aquaculture practices. Here, using 16S rRNA gene high-throughput sequencing, we explored the microbiome of farmed seabream to assess similarities and differences among microbial assemblages associated to different tissues and compare them with those in the surrounding environment. Seabream had distinct associated microbiomes according to the tissue and compared to the marine environment. The gut hosted the most diverse microbiome; different sets of dominant ASVs characterized the environmental and fish samples. The similarity between fish and environmental microbiomes was higher in seawater than sediment (up to 7.8 times), and the highest similarity (3.9%) was observed between gill and seawater, suggesting that gills are more closely interacting with the environment. We finally analyzed the potential connections occurring among microbiomes. These connections were relatively low among the host’s tissues and, in particular, between the gut and the others fish-related microbiomes; other tissues, including skin and gills, were found to be the most connected microbiomes. Our results suggest that, in mariculture, seabream microbiomes reflect only partially those in their surrounding environment and that the host is the primary driver shaping the seabream microbiome. These data provide a step forward to understand the role of the microbiome in farmed fish and farming environments, useful to enhance disease control, fish health, and environmental sustainability.

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Microbiological changes, shelf life and identification of initial and spoilage microbiota of sea bream fillets stored under various conditions using 16S rRNA gene analysis

Cited by 70 publications

References 62 publications

The dynamics of Pseudomonas and volatilome during the spoilage of gutted sea bream stored at 2 °C

The dynamics of Pseudomonas and volatilome during the spoilage of gutted sea bream stored at 2 °C

Microbiological status based on 454-pyrosequencing and volatilome analysis of gilthead seabream (Sparus aurata) fed on diets with hydrolyzed feather meal and poultry by-product meal as fishmeal replacers

Host-associated and Environmental Microbiomes in an Open-Sea Mediterranean Gilthead Sea Bream Fish Farm

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