Microbiome and Vulvovaginitis

Powell, Ann‐Marie; Sarria, Isabella; Goje, Oluwatosin

doi:10.1016/j.ogc.2023.02.005

Cited by 7 publications

(5 citation statements)

References 57 publications

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“…Additionally, the relative abundance of the various species identified by this test mirrors what has been reported [2,25,32,40,41], with Lactobacillus sp. (except L. acidophilus ), G. vaginalis, A.…”

Section: Discussionsupporting

confidence: 82%

“…These species are L. crispatus, L. jensenii, L. gasseri, L. iners, L. acidophilia, Gardnerella vaginalis, Fannyhessea vaginae (Atopobium vaginae), Megasphaera sp. types 1 and 2, Prevotella bivia, Bacterial Vaginosis-Associated Bacterium (BVAB) 1-3, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium, Mobiluncus curtisii, Mobiluncus mulieris, Sneathia sanguinegens, Bifidobacterium breve, Bacteroides fragilis, and Streptococcus anginosus [19,[23][24][25].…”

Section: Targeted Bacterial Speciesmentioning

confidence: 99%

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Molecular Characterization of Vaginal Microbiota Using a New 22-Species qRT-PCR Test to Achieve a Relative-abundance and Species-based Diagnosis of Bacterial Vaginosis

Oyenihi,

Haines,

Trama

et al. 2024

Preprint

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Background. Numerous bacteria are involved in the etiology of bacterial vaginosis (BV). Yet, current tests only focus on a select few. We therefore designed a new test targeting 22 BV-relevant species. Methods. Using 946 stored vaginal samples, a new qPCR test that quantitatively identifies 22 bacterial species was designed. The distribution and relative abundance of each species, α- and β-diversities, correlation and species co-existence were determined per sample. A diagnostic index was modeled from the data, trained, and tested to classify samples into BV-positive, BV-negative, or transitional BV. Results. The qPCR test identified all 22 targeted species with 95 to 100% sensitivity and specificity within 8 hours (from sample reception). Across most samples, Lactobacillus iners, Lactobacillus crispatus, Lactobacillus jensenii, Gardnerella vaginalis, Fannyhessea (Atopobium) vaginae, Prevotella bivia, and Megasphaera sp. type 1 were relatively abundant. BVAB-1 was more abundant and distributed than BVAB-2 and BVAB-3. No Mycoplasma genitalium was found. Inter-sample similarity was very low, and correlations existed between key species, which were used to model, train, and test a diagnostic index: MDL-BV index. The MDL-BV index, using both species and relative abundance markers, classified samples into three vaginal microbiome states. Testing this index on our samples, 491 were BV-positive, 318 were BV-negative, and 137 were transitional BV. Although important differences in BV status were observed between different age groups, races, and pregnancy status, they were statistically insignificant. Conclusion. Using a diverse and large number of vaginal samples from different races and age groups, including pregnant women, the new qRT-PCR test and MDL-BV index efficiently diagnosed BV within 8 hours (from sample reception), using 22 BV-associated species.

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Section: Discussionsupporting

confidence: 82%

Section: Targeted Bacterial Speciesmentioning

confidence: 99%

Molecular Characterization of Vaginal Microbiota Using a New 22-Species qRT-PCR Test to Achieve a Relative-abundance and Species-based Diagnosis of Bacterial Vaginosis

Oyenihi,

Haines,

Trama

et al. 2024

Preprint

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show abstract

“…Additionally, the relative abundance of the various species identified by this test mirrors what has been reported (Malaguti et al, 2015;Bayigga et al, 2019;Zhang et al, 2022;Powell et al, 2023), with Lactobacillus sp. (except L. acidophilus), G. vaginalis, A.…”

Section: Figure 13supporting

confidence: 79%

“…These species are L. crispatus, L. jensenii, L. gasseri, L. iners, L. acidophilus, Gardnerella vaginalis, Fannyhessea vaginae (Atopobium vaginae), Megasphaera sp. types 1 and 2, Prevotella bivia, Bacterial Vaginosis-Associated Bacterium (BVAB) 1-3, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium, Mobiluncus curtisii, Mobiluncus mulieris, Sneathia sanguinegens, Bifidobacterium breve, Bacteroides fragilis, and Streptococcus anginosus (Onderdonk et al, 2016;Muzny et al, 2020;Mondal et al, 2023;Powell et al, 2023).…”

Section: Targeted Bacterial Speciesmentioning

confidence: 99%

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“…While it is generally caused by infection, this, especially after menopause, may be the consequence of a hormonal imbalance [2]. Typical symptoms are burning, pruritus, dyspareunia, pain, leucoxanthorrhea, and vaginal discharge [3].…”

Section: Introductionmentioning

confidence: 99%

Efficacy of a Novel Rigenase® and Polyhexanide (Fitostimoline® Septagel) Hydrogel Device for the Treatment of Vulvovaginitis Symptoms: Cross-Sectional Analysis of a National Survey and Prospective Observational Study

Riemma,

Mainini,

Lukanović

et al. 2023

Medicina

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Background and Objectives: Signs and symptoms of vulvovaginitis, especially when recurrent, have a significant impact on a woman’s quality of life. The aim of this study was to survey gynecologists about their habits regarding the treatments of the pathology and to evaluate the efficacy of a novel vaginal hydrogel composed of wheat extracts and polyhexanide aimed at reducing vulvovaginitis symptomatology. Materials and Methods: A cross-sectional analysis of a national survey using 155 Italian gynecologists and a prospective, open-label, observational study were carried out in 75 outpatient clinics across Italy. Pre- and postmenopausal women with suspicion of vulvovaginitis due to at least four of the following symptoms (leucoxanthorrhea, bad odor from genitalia, vulvovaginal dryness, petechiae, burning, and pruritus) while waiting for microbiological swab analysis were included and treated with one hydrogel application every 3 days for 1 week. Primary endpoint was the complete resolution of symptomatology. Results: The pre-study survey reported that, for most clinicians, local or oral treatment (65.7% and 82.8%, respectively) with antibiotics or antifungals is used very often. Therefore, we proceeded to carry out an observational study. Overall, 615 (362 of fertile age and 253 in postmenopause) women were included in this study. At the 28th follow-up examination, complete resolution of symptomatology was achieved in 578/615 (94.1%; p < 0.001) within 12.72 ± 6.55 and 13.22 ± 6.33 days for those of fertile age and in postmenopause, respectively (p = 0.342). All of the evaluated symptoms were significantly reduced after treatment (p = 0.001) without differences according to the patient’s menopausal status. A slightly significant reduction in Gardnerella Vaginalis (p = 0.040) and Candida Albicans (p = 0.049) was found after treatment. No patient reported side effects, adverse reactions, or discontinued therapy. Conclusions: This pilot study showed that a hydrogel based on Rigenase® (wheat extract) and polyhexanide could be a promising treatment for the relief of vulvovaginitis symptoms. However, these results are limited by the absence of a control group. Additional comparative and randomized controlled trials between the hydrogel and other non-antibiotic devices as well as local antibiotic therapy should be performed to increase the validity of the findings.

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Microbiome and Vulvovaginitis

Cited by 7 publications

References 57 publications

Molecular Characterization of Vaginal Microbiota Using a New 22-Species qRT-PCR Test to Achieve a Relative-abundance and Species-based Diagnosis of Bacterial Vaginosis

Molecular Characterization of Vaginal Microbiota Using a New 22-Species qRT-PCR Test to Achieve a Relative-abundance and Species-based Diagnosis of Bacterial Vaginosis

DataSheet_1.pdf

Efficacy of a Novel Rigenase® and Polyhexanide (Fitostimoline® Septagel) Hydrogel Device for the Treatment of Vulvovaginitis Symptoms: Cross-Sectional Analysis of a National Survey and Prospective Observational Study

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